Discrimination of toxic impacts of various chemicals using chemical–gene expression profiling of Escherichia coli DNA microarray

2007 ◽  
Vol 42 (3) ◽  
pp. 392-400 ◽  
Author(s):  
Byoung Chan Kim ◽  
Man Bock Gu
Keyword(s):  
Gene Expression ◽  
Escherichia Coli ◽  
Gene Expression Profiling ◽  
Dna Microarray ◽  
Expression Profiling

scholarly journals Perceiving molecular evolution processes in Escherichia coli by comprehensive metabolite and gene expression profiling

2008 ◽  
Vol 9 (4) ◽  
pp. R72 ◽  
Author(s):  
Chandran Vijayendran ◽  
Aiko Barsch ◽  
Karl Friehs ◽  
Karsten Niehaus ◽  
Anke Becker ◽  
...  
Keyword(s):  
Gene Expression ◽  
Escherichia Coli ◽  
Molecular Evolution ◽  
Gene Expression Profiling ◽  
Expression Profiling

scholarly journals Complex Physiology and Compound Stress Responses during Fermentation of Alkali-Pretreated Corn Stover Hydrolysate by an Escherichia coli Ethanologen

2012 ◽  
Vol 78 (9) ◽  
pp. 3442-3457 ◽  
Author(s):  
Michael S. Schwalbach ◽  
David H. Keating ◽  
Mary Tremaine ◽  
Wesley D. Marner ◽  
Yaoping Zhang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Escherichia Coli ◽  
Amino Acids ◽  
Stationary Phase ◽  
Gene Expression Profiling ◽  
Stress Responses ◽  
Expression Profiling ◽  
Content Type ◽  
E Coli ◽  
Cell Maintenance

ABSTRACTThe physiology of ethanologenicEscherichia coligrown anaerobically in alkali-pretreated plant hydrolysates is complex and not well studied. To gain insight into howE. coliresponds to such hydrolysates, we studied anE. coliK-12 ethanologen fermenting a hydrolysate prepared from corn stover pretreated by ammonia fiber expansion. Despite the high sugar content (∼6% glucose, 3% xylose) and relatively low toxicity of this hydrolysate,E. coliceased growth long before glucose was depleted. Nevertheless, the cells remained metabolically active and continued conversion of glucose to ethanol until all glucose was consumed. Gene expression profiling revealed complex and changing patterns of metabolic physiology and cellular stress responses during an exponential growth phase, a transition phase, and the glycolytically active stationary phase. During the exponential and transition phases, high cell maintenance and stress response costs were mitigated, in part, by free amino acids available in the hydrolysate. However, after the majority of amino acids were depleted, the cells entered stationary phase, and ATP derived from glucose fermentation was consumed entirely by the demands of cell maintenance in the hydrolysate. Comparative gene expression profiling and metabolic modeling of the ethanologen suggested that the high energetic cost of mitigating osmotic, lignotoxin, and ethanol stress collectively limits growth, sugar utilization rates, and ethanol yields in alkali-pretreated lignocellulosic hydrolysates.

Gene expression profiling in streptozotocin treated mouse liver using DNA microarray

2005 ◽  
Vol 56 (4-5) ◽  
pp. 235-244 ◽  
Author(s):  
Eisuke Kume ◽  
Chinami Aruga ◽  
Yukito Ishizuka ◽  
Kaori Takahashi ◽  
Satoko Miwa ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Dna Microarray ◽  
Expression Profiling ◽  
Mouse Liver ◽  
Treated Mouse
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