Production of acetone–butanol–ethanol (ABE) in a continuous flow bioreactor using degermed corn and Clostridium beijerinckii

2007 ◽  
Vol 42 (1) ◽  
pp. 34-39 ◽  
Author(s):  
Thaddeus Ezeji ◽  
Nasib Qureshi ◽  
Hans P. Blaschek
Keyword(s):  
Continuous Flow ◽  
Clostridium Beijerinckii ◽  
Acetone Butanol Ethanol

scholarly journals Production of butyric acid at constant pH by a solventogenic strain of Clostridium beijerinckii

2020 ◽  
Vol 38 (No. 3) ◽  
pp. 185-191 ◽  
Author(s):  
Marek Drahokoupil ◽  
Petra Patáková
Keyword(s):  
Flow Cytometry ◽  
Butyric Acid ◽  
Population Viability ◽  
Clostridium Beijerinckii ◽  
Clostridium Tyrobutyricum ◽  
Fluorescence Staining ◽  
Low Concentration ◽  
Ph Values ◽  
Constant Ph ◽  
Acetone Butanol Ethanol

A solventogenic strain of Clostridium beijerinckii, NRRL B-598, was cultured for the production of butyric acid as the main fermentation product. However, unlike typical acetone-butanol-ethanol (ABE) fermentations, where pH is not regulated, in this study the pH was kept constant during fermentation. From the five pH values tested, 6.0, 6.5, 7.0, 7.5 and 8.0, pH 6.5 and 7.0 resulted in the highest concentrations of butyric acid, at 9.69 ± 0.09 g L–1 and 11.5 ± 0.39 g L–1, respectively. However, a low concentration of solvents, 1.8 ± 0.22 g L–1, was only reached at pH 7.0. These results are comparable with those from typical butyric acid producers, i.e. Clostridium butyricum and Clostridium tyrobutyricum strains. At pH 7.0, we succeeded in suppressing sporulation and prolonging the population viability, which was confirmed by flow cytometry combined with double fluorescence staining.

scholarly journals Milling byproducts are an economically viable substrate for butanol production using clostridial ABE fermentation

2020 ◽  
Vol 104 (20) ◽  
pp. 8679-8689
Author(s):  
Nils Thieme ◽  
Johanna C. Panitz ◽  
Claudia Held ◽  
Birgit Lewandowski ◽  
Wolfgang H. Schwarz ◽  
...  
Keyword(s):  
Clostridium Beijerinckii ◽  
Abe Fermentation ◽  
Liquid Fuels ◽  
Butanol Production ◽  
Enzymatic Pretreatment ◽  
Profitability Analysis ◽  
Key Points ◽  
Wide Range ◽  
Wheat Middlings ◽  
Acetone Butanol Ethanol

Abstract Butanol is a platform chemical that is utilized in a wide range of industrial products and is considered a suitable replacement or additive to liquid fuels. So far, it is mainly produced through petrochemical routes. Alternative production routes, for example through biorefinery, are under investigation but are currently not at a market competitive level. Possible alternatives, such as acetone-butanol-ethanol (ABE) fermentation by solventogenic clostridia are not market-ready to this day either, because of their low butanol titer and the high costs of feedstocks. Here, we analyzed wheat middlings and wheat red dog, two wheat milling byproducts available in large quantities, as substrates for clostridial ABE fermentation. We could identify ten strains that exhibited good butanol yields on wheat red dog. Two of the best ABE producing strains, Clostridium beijerinckii NCIMB 8052 and Clostridium diolis DSM 15410, were used to optimize a laboratory-scale fermentation process. In addition, enzymatic pretreatment of both milling byproducts significantly enhanced ABE production rates of the strains C. beijerinckii NCIMB 8052 and C. diolis DSM 15410. Finally, a profitability analysis was performed for small- to mid-scale ABE fermentation plants that utilize enzymatically pretreated wheat red dog as substrate. The estimations show that such a plant could be commercially successful. Key points • Wheat milling byproducts are suitable substrates for clostridial ABE fermentation. • Enzymatic pretreatment of wheat red dog and middlings increases ABE yield. • ABE fermentation plants using wheat red dog as substrate are economically viable.

Butanol production from the effluent of hydrogen fermentation

2011 ◽  
Vol 63 (6) ◽  
pp. 1236-1240 ◽  
Author(s):  
W. H. Chen ◽  
S. Y. Chen ◽  
S. J. Chao ◽  
Z. C. Jian
Keyword(s):  
Consumption Rate ◽  
Clostridium Beijerinckii ◽  
Abe Fermentation ◽  
Acetate Buffer ◽  
Microbial Populations ◽  
Butanol Production ◽  
Practical Application ◽  
Carbohydrate Consumption ◽  
Acetone Butanol Ethanol ◽  
Acetate Butyrate

The purpose of the study was to recover butanol from the effluent of the hydrogen-producing bioreactor containing acetate, butyrate, and carbohydrate. The butanol production by Clostridium beijerinckii NRRL B592 was evaluated under both unsterilized and sterilized conditions for examining the potential of butanol production for the practical application. Sucrose of 10 g/L and butyrate of 2 g/L coupled with acetate buffer were used to mimic the effluent. Sucrose was completely consumed in the both unsterilized and sterilized conditions during acetone-butanol-ethanol (ABE) fermentation. However, the results illustrate that the carbohydrate consumption rate in the unsterilized condition was higher than that in the sterilized condition. The maximum butanol concentrations of 3,500 and 3,750 mg/L were achieved in the sterilized and unsterilized conditions, respectively. Meanwhile, it was found that the acetate and the butyrate concentrations of 600 and 1,500 mg/L, and 300 and 1,000 mg/L were ingested to yield butanol in the sterilized condition and in the unsterilized condition, respectively. The results concluded that high levels of acetate and butyrate could eliminate the interference of other microbial populations, resulting in the enrichment of C. beijerinckii NRRL B592 in the fermentor. The butanol production by C. beijerinckii NRRL B592 could be, therefore, produced from the effluent of the hydrogen-producing bioreactor. It promised that the microbial butanol production is one of attractive bioprocesses to recover energy from wastes.

Acetone–butanol–ethanol production in a novel continuous flow system

2015 ◽  
Vol 190 ◽  
pp. 315-320 ◽  
Author(s):  
Elsayed Elbeshbishy ◽  
Bipro Ranjan Dhar ◽  
Hisham Hafez ◽  
Hyung-Sool Lee
Keyword(s):  
Ethanol Production ◽  
Continuous Flow ◽  
Flow System ◽  
Continuous Flow System ◽  
Acetone Butanol Ethanol

Organosolv pretreated beech wood as a substrate for acetone butanol ethanol extractive fermentation

Holzforschung ◽  
2018 ◽  
Vol 73 (1) ◽  
pp. 55-64
Author(s):  
Helena González-Peñas ◽  
Thelmo A. Lú-Chau ◽  
Nicolás Botana ◽  
María Teresa Moreira ◽  
Juan M. Lema ◽  
...  
Keyword(s):  
Beech Wood ◽  
Product Inhibition ◽  
Clostridium Beijerinckii ◽  
Future Research ◽  
Total Production ◽  
Extractive Fermentation ◽  
Extraction Solvent ◽  
Total Productivity ◽  
Cellulose Hydrolysate ◽  
Acetone Butanol Ethanol

Abstract Acetone-butanol-ethanol (ABE) fermentation has a high potential for industrial application in lignocellulose-based biorefinery. Nevertheless, strong end-product inhibition is a severe limitation of this process, which can be partially solved by in situ product recovery techniques, such as extractive fermentation. In this work, the hydrolyzed cellulose from organosolv pre-treated beech wood (Fagus sylvatica L.) was fermented by Clostridium beijerinckii CECT 508 and compared with synthetic P2 medium in conventional and extractive fermentation processes. The conventional control fermentation yielded 9.12 g l−1 of ABE with C. beijerinckii CECT 508, while ABE overproduction of 40% was observed with cellulose hydrolysate as a substrate. After extractive batch fermentation based on 2-butyl-1-octanol as extractant, the final ABE titer was doubled in the P2 control medium over the conventional batch, with respective increases of 50, 190, and 140% for butanol (B), acetone (A) and ethanol (E). In the case of ABE as a hydrolysate medium, the total production increased by 10% with liquid extraction, while mainly B was responsible for this improvement. The total productivity was nevertheless gradually decreased in extractive fermentation, with a significantly slower production rate and the microbial growth compared to conventional fermentation. Moreover, solvent production and sugar consumption stopped, although the aqueous B concentration was below the inhibition threshold. The mid-term toxicity of the extraction solvent 2-butyl-1-octanol seems responsible for these results and this issue should be considered in future research.

Enhanced Acetone-Butanol-Ethanol Production by Clostridium beijerinckii Using Biochar

2019 ◽  
Author(s):  
Xiao Sun ◽  
Hasan K. Atiyeh ◽  
Yinka A. Adesanya ◽  
Hailin Zhang ◽  
Christopher Okonkwo ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Clostridium Beijerinckii ◽  
Acetone Butanol Ethanol
Sign in / Sign up

Export Citation Format

Share Document