Improving lipase production by nutrient source modification using Rhizopus homothallicus cultured in solid state fermentation

2006 ◽  
Vol 41 (11) ◽  
pp. 2264-2269 ◽  
Author(s):  
J.A. Rodriguez ◽  
J.C. Mateos ◽  
J. Nungaray ◽  
V. González ◽  
T. Bhagnagar ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Lipase Production ◽  
Nutrient Source ◽  
Rhizopus Homothallicus

scholarly journals OIL CAKES AS SUBSTRATE FOR IMPROVED LIPASE PRODUCTION IN SOLID STATE FERMENTATION

2011 ◽  
Vol 3 (1) ◽  
pp. 71-73 ◽  
Author(s):  
JAYA BHAGAT ◽  
◽  
PRATYOOSH SHUKLA ◽  
SMRITI SHRIVASTAVA
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Lipase Production ◽  
Oil Cakes

scholarly journals Optimization of Lipase Production in Solid-State Fermentation by Rhizopus Arrhizus in Nutrient Medium Containing Agroindustrial Wastes

2018 ◽  
Vol 12 (1) ◽  
pp. 189-203 ◽  
Author(s):  
Georgi Dobrev ◽  
Hristina Strinska ◽  
Anelia Hambarliiska ◽  
Boriana Zhekova ◽  
Valentina Dobreva
Keyword(s):  
Solid State ◽  
Moisture Content ◽  
Solid State Fermentation ◽  
Lipase Activity ◽  
Nutrient Medium ◽  
Solid Medium ◽  
Low Cost ◽  
Lipase Production ◽  
Rhizopus Arrhizus ◽  
Agroindustrial Wastes

Background: Rhizopus arrhizus is a potential microorganism for lipase production. Solid-state fermentation is used for microbial biosynthesis of enzymes, due to advantages, such as high productivity, utilization of abundant and low-cost raw materials, and production of enzymes with different catalytic properties. Objective: The objective of the research is optimization of the conditions for lipase production in solid-state fermentation by Rhizopus arrhizus in a nutrient medium, containing agroindustrial wastes. Method: Biosynthesis of lipase in solid-state fermentation by Rhizopus arrhizus was investigated. The effect of different solid substrates, additional carbon and nitrogen source, particles size and moisture content of the medium on enzyme production was studied. Response surface methodology was applied for determination of the optimal values of moisture content and tryptone concentration. A procedure for efficient lipase extraction from the fermented solids was developed. Results: Highest lipase activity was achieved when wheat bran was used as a solid substrate. The addition of 1% (w/w) glucose and 5% (w/w) tryptone to the solid medium significantly increased lipase activity. The structure of the solid medium including particles size and moisture content significantly influenced lipase production. A mathematical model for the effect of moisture content and tryptone concentration on lipase activity was developed. Highest enzyme activity was achieved at 66% moisture and 5% (w/w) tryptone. The addition of the non-ionic surfactant Disponyl NP 3070 in the eluent for enzyme extraction from the fermented solids increased lipase activity about three folds. Conclusion: After optimization of the solid-state fermentation the achieved 1021.80 U/g lipase activity from Rhizopus arrhizus was higher and comparable with the activity of lipases, produced by other fungal strains. The optimization of the conditions and the use of low cost components in solid-state fermentation makes the process economicaly effective for production of lipase from the investigated strain Rhizopus arrhizus.

Multipurpose fixed-bed bioreactor to simplify lipase production by solid-state fermentation and application in biocatalysis

2019 ◽  
Vol 144 ◽  
pp. 1-7 ◽  
Author(s):  
Sabrini N.S. Ávila ◽  
Melissa L.E. Gutarra ◽  
Roberto Fernandez-Lafuente ◽  
Elisa D.C. Cavalcanti ◽  
Denise M.G. Freire
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Fixed Bed ◽  
Lipase Production ◽  
Fixed Bed Bioreactor

Artificial neural network hybridized with a genetic algorithm for optimization of lipase production from Penicillium roqueforti ATCC 10110 in solid-state fermentation

2021 ◽  
Vol 31 ◽  
pp. 101885
Author(s):  
Luiz Henrique Sales de Menezes ◽  
Lucas Lima Carneiro ◽  
Iasnaia Maria de Carvalho Tavares ◽  
Pedro Henrique Santos ◽  
Thiago Pereira das Chagas ◽  
...  
Keyword(s):  
Neural Network ◽  
Genetic Algorithm ◽  
Artificial Neural Network ◽  
Solid State ◽  
Solid State Fermentation ◽  
Lipase Production ◽  
Penicillium Roqueforti ◽  
Artificial Neural

Lipase production by Penicillium restrictum in solid-state fermentation using babassu oil cake as substrate

1999 ◽  
Vol 35 (1-2) ◽  
pp. 85-90 ◽  
Author(s):  
Andreas K Gombert ◽  
Annette L Pinto ◽  
Leda R Castilho ◽  
Denise M.G Freire
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Lipase Production ◽  
Babassu Oil ◽  
Penicillium Restrictum

Lipase production by solid state fermentation of olive cake and sugar cane bagasse

1998 ◽  
Vol 5 (1-4) ◽  
pp. 75-78 ◽  
Author(s):  
J Cordova ◽  
M Nemmaoui ◽  
M Ismaı̈li-Alaoui ◽  
A Morin ◽  
S Roussos ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Sugar Cane ◽  
Lipase Production ◽  
Sugar Cane Bagasse ◽  
Olive Cake

Lipase Production by Solid-State Fermentation: Cultivation Conditions and Operation of Tray and Packed-Bed Bioreactors

2005 ◽  
Vol 121 (1-3) ◽  
pp. 0105-0116 ◽  
Author(s):  
Melissa L. E. Gutarra ◽  
Elisa D. C. Cavalcanti ◽  
Leda R. Castilho ◽  
Denise M. G. Freire ◽  
Geraldo L. Sant'Anna
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Packed Bed ◽  
Lipase Production ◽  
Cultivation Conditions
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