Alterations in physicochemical properties and bile acid binding capacities of dietary fibers upon ultrafine grinding

2018 ◽  
Vol 326 ◽  
pp. 146-150 ◽  
Author(s):  
Kehao Huang ◽  
Bin Du ◽  
Baojun Xu
Keyword(s):  
Bile Acid ◽  
Physicochemical Properties ◽  
Dietary Fibers ◽  
Ultrafine Grinding ◽  
Bile Acid Binding

Intestinal interaction of bile acids, phospholipids, dietary fibers, and cholestyramine

1986 ◽  
Vol 250 (4) ◽  
pp. G420-G426 ◽  
Author(s):  
D. Gallaher ◽  
B. O. Schneeman
Keyword(s):  
Bile Acid ◽  
Bile Acids ◽  
Aqueous Phase ◽  
Guar Gum ◽  
Binding Capacity ◽  
Dietary Fibers ◽  
Bile Acid Concentration ◽  
Oat Bran ◽  
Intestinal Contents ◽  
Bile Acid Binding

Binding of bile acids and phospholipids to a number of dietary fibers and cholestyramine (CH) within the small intestine was determined. The fibers used were cellulose, wheat bran, oat bran, guar gum (GG), and lignin (LG). GG, LG, and CH bound significant quantities of bile acids. However, only the CH reduced the bile acid concentration within the aqueous phase of the intestinal contents. Significant phospholipid binding was found only with CH. None of the test substances significantly reduced the quantity of solubilized lipid. Multiple regression analysis indicated that the total quantity of bile acids and phospholipids in the aqueous phase of the intestinal contents was a significant predictor of the quantity of lipid solubilized within the contents (r2 = 0.67). The failure of GG and LG to significantly decrease the amount of solubilized lipid suggests that the hypocholesterolemic effect of these fibers is due more to their bile acid binding capacity than to an effect on lipid solubilization.

scholarly journals Plasma cholesterol lowering action of bile acid binding polymers in experimental animals

1960 ◽  
Vol 1 (5) ◽  
pp. 469-473 ◽  
Author(s):  
DavidM. Tennent ◽  
Henry Siegel ◽  
MaryE. Zanetti ◽  
GuntherW. Kuron ◽  
WaltherH. Ott ◽  
...  
Keyword(s):  
Bile Acid ◽  
Plasma Cholesterol ◽  
Cholesterol Lowering ◽  
Experimental Animals ◽  
Bile Acid Binding

scholarly journals Validation of Recombinant Chicken Liver Bile Acid Binding Protein as a Tool for Cholic Acid Hosting

Biomolecules ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 645
Author(s):  
Giusy Tassone ◽  
Maurizio Orlandini ◽  
Massimo Olivucci ◽  
Cecilia Pozzi
Keyword(s):  
Bile Acid ◽  
High Resolution ◽  
Affinity Chromatography ◽  
Bile Acids ◽  
Drug Carriers ◽  
Chicken Liver ◽  
Purification Procedure ◽  
Acid Binding Protein ◽  
Bile Acid Binding ◽  
Exogenous Substances

Bile acids (BAs) are hydroxylated steroids derived from cholesterol that act at the intestinal level to facilitate the absorption of several nutrients and also play a role as signaling molecules. In the liver of various vertebrates, the trafficking of BAs is mediated by bile acid-binding proteins (L-BABPs). The ability to host hydrophobic or amphipathic molecules makes BABPs suitable for the distribution of a variety of physiological and exogenous substances. Thus, BABPs have been proposed as drug carriers, and more recently, they have also been employed to develop innovative nanotechnology and biotechnology systems. Here, we report an efficient protocol for the production, purification, and crystallization of chicken liver BABP (cL-BABP). By means of target expression as His6-tag cL-BABP, we obtained a large amount of pure and homogeneous proteins through a simple purification procedure relying on affinity chromatography. The recombinant cL-BABP showed a raised propensity to crystallize, allowing us to obtain its structure at high resolution and, in turn, assess the structural conservation of the recombinant cL-BABP with respect to the liver-extracted protein. The results support the use of recombinant cL-BABP for the development of drug carriers, nanotechnologies, and innovative synthetic photoswitch systems.

Structural and dynamic roles of permanent water molecules in ligand molecular recognition by chicken liver bile acid binding protein

2008 ◽  
Vol 21 (5) ◽  
pp. 348-354 ◽  
Author(s):  
Piero Ricchiuto ◽  
Alessandro Guerini Rocco ◽  
Elisabetta Gianazza ◽  
Dario Corrada ◽  
Tiziana Beringhelli ◽  
...  
Keyword(s):  
Bile Acid ◽  
Molecular Recognition ◽  
Binding Protein ◽  
Chicken Liver ◽  
Water Molecules ◽  
Acid Binding Protein ◽  
Bile Acid Binding

NMR unfolding studies on a liver bile acid binding protein reveal a global two-state unfolding and localized singular behaviors

2009 ◽  
Vol 481 (1) ◽  
pp. 21-29 ◽  
Author(s):  
Mariapina D’Onofrio ◽  
Laura Ragona ◽  
Dimitrios Fessas ◽  
Marco Signorelli ◽  
Raffaella Ugolini ◽  
...  
Keyword(s):  
Bile Acid ◽  
Binding Protein ◽  
Acid Binding Protein ◽  
Bile Acid Binding

scholarly journals Identification of a Bile Acid-responsive Element in the Human Ileal Bile Acid-binding Protein Gene

1999 ◽  
Vol 274 (42) ◽  
pp. 29749-29754 ◽  
Author(s):  
Jacques Grober ◽  
Isabelle Zaghini ◽  
Hiroshi Fujii ◽  
Stacey A. Jones ◽  
Steven A. Kliewer ◽  
...  
Keyword(s):  
Bile Acid ◽  
Protein Gene ◽  
Binding Protein ◽  
Acid Binding Protein ◽  
Bile Acid Binding ◽  
Binding Protein Gene ◽  
Responsive Element
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