Collagen and chitosan blends for 3D bioprinting: A rheological and printability approach

2020 ◽  
Vol 82 ◽  
pp. 106297 ◽  
Author(s):  
Ana Carolina Heidenreich ◽  
Mercedes Pérez-Recalde ◽  
Ana González Wusener ◽  
Élida Beatriz Hermida
Keyword(s):  
3D Bioprinting ◽  
Chitosan Blends

scholarly journals Motility Improvement of Biomimetic Trachea Scaffold via Hybrid 3D-Bioprinting Technology

Polymers ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 971
Author(s):  
Young Soo Yu ◽  
Chi Bum Ahn ◽  
Kuk Hui Son ◽  
Jin Woo Lee
Keyword(s):  
Rotation Angle ◽  
Thick Layer ◽  
Cartilage Tissue ◽  
Soft Tissue Reconstruction ◽  
3D Bioprinting ◽  
Rotating Beam ◽  
Rotational Angle ◽  
Gelatin Hydrogel ◽  
Tissue Reconstruction ◽  
Neck Rotation

A trachea has a structure capable of responding to various movements such as rotation of the neck and relaxation/contraction of the conduit due to the mucous membrane and cartilage tissue. However, current reported tubular implanting structures are difficult to impelement as replacements for original trachea movements. Therefore, in this study, we developed a new trachea implant with similar anatomical structure and mechanical properties to native tissue using 3D printing technology and evaluated its performance. A 250 µm-thick layer composed of polycaprolactone (PCL) nanofibers was fabricated on a rotating beam using electrospinning technology, and a scaffold with C-shaped cartilage grooves that mimics the human airway structure was printed to enable reconstruction of cartilage outside the airway. A cartilage type scaffold had a highest rotational angle (254°) among them and it showed up to 2.8 times compared to human average neck rotation angle. The cartilage type showed a maximum elongation of 8 times higher than that of the bellows type and it showed the elongation of 3 times higher than that of cylinder type. In cartilage type scaffold, gelatin hydrogel printed on the outside of the scaffold was remain 22.2% under the condition where no hydrogel was left in other type scaffolds. In addition, after 2 days of breathing test, the amount of gelatin remaining inside the scaffold was more than twice that of other scaffolds. This novel trachea scaffold with hydrogel inside and outside of the structure was well-preserved under external flow and is expected to be advantageous for soft tissue reconstruction of the trachea.

scholarly journals A 3D Bioprinted Material That Recapitulates the Perivascular Bone Marrow Structure for Sustained Hematopoietic and Cancer Models

Polymers ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 480
Author(s):  
Caitlyn A. Moore ◽  
Zain Siddiqui ◽  
Griffin J. Carney ◽  
Yahaira Naaldijk ◽  
Khadidiatou Guiro ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Bone Marrow ◽  
Translational Medicine ◽  
Drug Response ◽  
Drug Approval ◽  
3D Models ◽  
3D Bioprinting ◽  
Effective Evaluation ◽  
Experimental Systems ◽  
Cancer Models

Translational medicine requires facile experimental systems to replicate the dynamic biological systems of diseases. Drug approval continues to lag, partly due to incongruencies in the research pipeline that traditionally involve 2D models, which could be improved with 3D models. The bone marrow (BM) poses challenges to harvest as an intact organ, making it difficult to study disease processes such as breast cancer (BC) survival in BM, and to effective evaluation of drug response in BM. Furthermore, it is a challenge to develop 3D BM structures due to its weak physical properties, and complex hierarchical structure and cellular landscape. To address this, we leveraged 3D bioprinting to create a BM structure with varied methylcellulose (M): alginate (A) ratios. We selected hydrogels containing 4% (w/v) M and 2% (w/v) A, which recapitulates rheological and ultrastructural features of the BM while maintaining stability in culture. This hydrogel sustained the culture of two key primary BM microenvironmental cells found at the perivascular region, mesenchymal stem cells and endothelial cells. More importantly, the scaffold showed evidence of cell autonomous dedifferentiation of BC cells to cancer stem cell properties. This scaffold could be the platform to create BM models for various diseases and also for drug screening.

scholarly journals 3D-Bioprinting Strategies Based on In Situ Bone-Healing Mechanism for Vascularized Bone Tissue Engineering

Micromachines ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 287
Author(s):  
Ye Lin Park ◽  
Kiwon Park ◽  
Jae Min Cha
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Bone Healing ◽  
Critical Size ◽  
Current Knowledge ◽  
3D Bioprinting ◽  
The Past ◽  
Regeneration Processes

Over the past decades, a number of bone tissue engineering (BTE) approaches have been developed to address substantial challenges in the management of critical size bone defects. Although the majority of BTE strategies developed in the laboratory have been limited due to lack of clinical relevance in translation, primary prerequisites for the construction of vascularized functional bone grafts have gained confidence owing to the accumulated knowledge of the osteogenic, osteoinductive, and osteoconductive properties of mesenchymal stem cells and bone-relevant biomaterials that reflect bone-healing mechanisms. In this review, we summarize the current knowledge of bone-healing mechanisms focusing on the details that should be embodied in the development of vascularized BTE, and discuss promising strategies based on 3D-bioprinting technologies that efficiently coalesce the abovementioned main features in bone-healing systems, which comprehensively interact during the bone regeneration processes.

scholarly journals Is Dialdehyde Chitosan a Good Substance to Modify Physicochemical Properties of Biopolymeric Materials?

2021 ◽  
Vol 22 (7) ◽  
pp. 3391
Author(s):  
Sylwia Grabska-Zielińska ◽  
Alina Sionkowska ◽  
Ewa Olewnik-Kruszkowska ◽  
Katarzyna Reczyńska ◽  
Elżbieta Pamuła
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Physicochemical Properties ◽  
Silk Fibroin ◽  
Three Dimensional ◽  
Microscopy Imaging ◽  
Maximum Deformation ◽  
One Step ◽  
Chitosan Blends ◽  
Fourier Transfer Infrared Spectroscopy

The aim of this work was to compare physicochemical properties of three dimensional scaffolds based on silk fibroin, collagen and chitosan blends, cross-linked with dialdehyde starch (DAS) and dialdehyde chitosan (DAC). DAS was commercially available, while DAC was obtained by one-step synthesis. Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis. Metabolic activity with AlamarBlue assay and live/dead fluorescence staining were performed to evaluate the cytocompatibility of the obtained materials with MG-63 osteoblast-like cells. The results showed that the properties of the scaffolds based on silk fibroin, collagen and chitosan can be modified by chemical cross-linking with DAS and DAC. It was found that DAS and DAC have different influence on the properties of biopolymeric scaffolds. Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC. Additionally, scaffolds cross-linked with DAS exhibited higher biocompatibility than those cross-linked with DAC. However, the obtained results showed that both types of scaffolds can provide the support required in regenerative medicine and tissue engineering. The scaffolds presented in the present work can be potentially used in bone tissue engineering to facilitate healing of small bone defects.

3D Bioprinting Constructs to Facilitate Skin Regeneration

2021 ◽  
pp. 2105080
Author(s):  
Luciana Y. Daikuara ◽  
Xifang Chen ◽  
Zhilian Yue ◽  
Danielle Skropeta ◽  
Fiona M. Wood ◽  
...  
Keyword(s):  
Skin Regeneration ◽  
3D Bioprinting

scholarly journals Rapid 3D BioPrinting of a human iPSC-derived cardiac micro-tissue for high-throughput drug testing

2021 ◽  
Vol 3 ◽  
pp. 100007
Author(s):  
Kathleen L. Miller ◽  
Yi Xiang ◽  
Claire Yu ◽  
Jacob Pustelnik ◽  
Jerry Wu ◽  
...  
Keyword(s):  
High Throughput ◽  
Drug Testing ◽  
3D Bioprinting ◽  
Human Ipsc

3D bioprinting for lung and tracheal tissue engineering: Criteria, advances, challenges, and future directions

Bioprinting ◽  
2021 ◽  
Vol 21 ◽  
pp. e00124
Author(s):  
Seyed Hossein Mahfouzi ◽  
Seyed Hamid Safiabadi Tali ◽  
Ghassem Amoabediny
Keyword(s):  
Tissue Engineering ◽  
3D Bioprinting ◽  
Future Directions ◽  
Tracheal Tissue

3D Bioprinting of Reinforced Vessels by Dual-Cross-linked Biocompatible Hydrogels

2021 ◽  
Author(s):  
Ke Peng ◽  
Xin Liu ◽  
Hao Zhao ◽  
Huan Lu ◽  
Fengting Lv ◽  
...  
Keyword(s):  
3D Bioprinting
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