Interruption of hydration state of thermoresponsive polymer, poly(N-isopropylacrylamide) in guanidinium hydrochloride

Polymer ◽  
2013 ◽  
Vol 54 (2) ◽  
pp. 791-797 ◽  
Author(s):  
P. Madhusudhana Reddy ◽  
Mohamed Taha ◽  
Awanish Kumar ◽  
Pannuru Venkatesu ◽  
Ming-Jer Lee
Keyword(s):  
Thermoresponsive Polymer ◽  
Guanidinium Hydrochloride ◽  
Hydration State

scholarly journals Effect of pore diameter on the elution behavior of analytes from thermoresponsive polymer grafted beads packed columns

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kenichi Nagase ◽  
Yuta Umemoto ◽  
Hideko Kanazawa
Keyword(s):  
Pore Diameter ◽  
Packed Column ◽  
Packed Columns ◽  
Butyl Methacrylate ◽  
Thermoresponsive Polymer ◽  
Thermoresponsive Polymers ◽  
Column Temperature ◽  
Pore Sizes ◽  
Temperature Responsive ◽  
Elution Behavior

AbstractTemperature-responsive chromatography using thermoresponsive polymers is innovative and can control analyte retention via column temperature. Analyte elution behavior in this type of chromatography depends on the modified thermoresponsive polymer and the structure of the base materials. In the present study, we examine the effect of the pore diameter of silica beads on analyte elution behavior in temperature-responsive chromatography. Poly(N-isopropylacrylamide-co-n-butyl methacrylate) hydrogel was applied to beads of various pore sizes: 7, 12, and 30 nm. Almost the same amount of copolymer hydrogel was applied to all beads, indicating that the efficiency of copolymer modification was independent of pore size. Analyte retention on prepared beads in a packed column was observed using steroids, benzodiazepines, and barbiturates as analytes. Analyte retention times increased with temperature on packed columns of 12- and 30-nm beads, whereas the column packed with 7-nm beads exhibited decreased retention times with increasing temperature. The difference in analyte elution behavior among the various pore sizes was attributed to analyte diffusion into the bead pores. These results demonstrate that bead pore diameter determines temperature-dependent elution behavior.

Highly precise characterization of the hydration state upon thermal denaturation of human serum albumin using a 65 GHz dielectric sensor

2020 ◽  
Vol 22 (35) ◽  
pp. 19468-19479 ◽  
Author(s):  
Keiichiro Shiraga ◽  
Mako Urabe ◽  
Takeshi Matsui ◽  
Shojiro Kikuchi ◽  
Yuichi Ogawa
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Thermal Denaturation ◽  
Hydration Water ◽  
Biological Functions ◽  
Dielectric Sensor ◽  
Hydration State ◽  
Precise Characterization

The biological functions of proteins depend on harmonization with hydration water surrounding them.

scholarly journals Solid–Liquid Interface Temperature Measurement of Evaporating Droplet Using Thermoresponsive Polymer Aqueous Solution

2021 ◽  
Vol 11 (8) ◽  
pp. 3379
Author(s):  
Hyung Ju Lee ◽  
Chan Ho Jeong ◽  
Dae Yun Kim ◽  
Chang Kyoung Choi ◽  
Seong Hyuk Lee
Keyword(s):  
Refractive Index ◽  
Heated Surface ◽  
Liquid Interface ◽  
Interface Temperature ◽  
Thermoresponsive Polymer ◽  
Temperature Deviation ◽  
Line Region ◽  
Optical And Mechanical Properties ◽  
Solid Liquid Interface ◽  
Solid Liquid

The present study aims to measure the solid–liquid interface temperature of an evaporating droplet on a heated surface using a thermoresponsive polymer. Poly(N-isopropylacrylamide) (pNIPAM) was used owing to its sensitive optical and mechanical properties to the temperature. We also measured the refractive index variation of the pNIPAM solution by using the surface plasmon resonance imaging (SPRi). In particular, the present study proposed a new method to measure the solid–liquid interface temperature using the correlation among reflectance, refractive index, and temperature. It was found that the reflectance of a pNIPAM solution decreased after the droplet deposition. The solid–liquid interface temperature, estimated from the reflectance, showed a lower value at the center of the droplet, and it gradually increased along the radial direction. The lowest temperature at the contact line region is present because of the maximum evaporative cooling. Moreover, the solid–liquid interface temperature deviation increased with the surface temperature, which means solid–liquid interface temperature should be considered at high temperature to predict the evaporation flux of the droplet accurately.

Diffusion-weighted MRI sequences (DW-MRI) of the kidney: normal findings, influence of hydration state and repeatability of results

2008 ◽  
Vol 113 (2) ◽  
pp. 214-224 ◽  
Author(s):  
M. B. Damasio ◽  
A. Tagliafico ◽  
E. Capaccio ◽  
C. Cancelli ◽  
N. Perrone ◽  
...  
Keyword(s):  
Diffusion Weighted Mri ◽  
Diffusion Weighted ◽  
Hydration State ◽  
Mri Sequences ◽  
Influence Of Hydration

Reversible Equilibrium Unfolding of Triosephosphate Isomerase fromTrypanosoma cruziin Guanidinium Hydrochloride Involves Stable Dimeric and Monomeric Intermediates†

Biochemistry ◽  
2005 ◽  
Vol 44 (32) ◽  
pp. 10883-10892 ◽  
Author(s):  
María Elena Chánez-Cárdenas ◽  
Gerardo Pérez-Hernández ◽  
Brenda Guadalupe Sánchez-Rebollar ◽  
Miguel Costas ◽  
Edgar Vázquez-Contreras
Keyword(s):  
Triosephosphate Isomerase ◽  
Guanidinium Hydrochloride ◽  
Equilibrium Unfolding

Modeling Swelling Behavior of Thermoresponsive Polymer Brush with Lattice Density Functional Theory

Langmuir ◽  
2014 ◽  
Vol 30 (14) ◽  
pp. 4040-4048 ◽  
Author(s):  
Cheng Lian ◽  
Le Wang ◽  
Xueqian Chen ◽  
Xia Han ◽  
Shuangliang Zhao ◽  
...  
Keyword(s):  
Density Functional Theory ◽  
Density Functional ◽  
Polymer Brush ◽  
Swelling Behavior ◽  
Thermoresponsive Polymer ◽  
Functional Theory ◽  
Lattice Density ◽  
Lattice Density Functional Theory

Regulation of Protein Binding toward a Ligand on Chromatographic Matrixes by Masking and Forced-Releasing Effects Using Thermoresponsive Polymer

2002 ◽  
Vol 74 (16) ◽  
pp. 4160-4166 ◽  
Author(s):  
Kimihiro Yoshizako ◽  
Yoshikatsu Akiyama ◽  
Hidenori Yamanaka ◽  
Yasuro Shinohara ◽  
Yukio Hasegawa ◽  
...  
Keyword(s):  
Protein Binding ◽  
Thermoresponsive Polymer
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