Rapid and quantitative detection of citrus huanglongbing bacterium ‘Candidatus Liberibacter asiaticus’ by real-time fluorescent loop-mediated isothermal amplification assay in China

2016 ◽  
Vol 94 ◽  
pp. 1-7 ◽  
Author(s):  
Xuhui Wu ◽  
Chunliang Meng ◽  
Guihua Wang ◽  
Yuanzheng Liu ◽  
Xin Zhang ◽  
...  
Keyword(s):  
Real Time ◽  
Isothermal Amplification ◽  
Quantitative Detection ◽  
Candidatus Liberibacter Asiaticus ◽  
Loop Mediated Isothermal Amplification ◽  
Candidatus Liberibacter ◽  
Citrus Huanglongbing ◽  
Amplification Assay ◽  
Liberibacter Asiaticus

scholarly journals Citrus huanglongbing: validation of Real-Time PCR (qPCR) for the detection of Candidatus Liberibacter asiaticus and Candidatus Liberibacter americanus in Colombia

2014 ◽  
Vol 32 (3) ◽  
pp. 377-389 ◽  
Author(s):  
Jorge Evelio Ángel ◽  
Erick Geovanni Hernández ◽  
Néstor Andrés Herrera ◽  
Linda Yhiset Gómez ◽  
Ángela Patricia Castro ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Detection Sensitivity ◽  
Candidatus Liberibacter Asiaticus ◽  
Plant Materials ◽  
Citrus Plant ◽  
Candidatus Liberibacter ◽  
Citrus Huanglongbing ◽  
Liberibacter Asiaticus ◽  
Reliability And Robustness

Citrus huanglongbing (HLB) is the most destructive citrus disease. Two of the three known HLB-associated CandidatusLiberibacter species were recently found to be present in the Americas. In this study, eggs, nymphs and adults of Diaphorinacitri Kuwayama (Hemiptera: Liviidae) and suspect citrus plant materials were collected in 25 municipalities in the departments of Cundinamarca, Santander, Valle del Cauca, Meta and Quindio (Colombia). The detection sensitivity, specificity and assay performance of the 16S rDNA-based real-time PCR (qPCR) were validated for the field survey of the disease in Colombia. The validation confirmed the reliability and robustness of the real-time PCR method for the detection of HLB bacteria in host citrus plant tissues and the vector D. citri. The diagnosis was performed for Candidatus Liberibacter asiaticus (Ca. L. asiaticus) and for Candidatus Liberibacter americanus (Ca. L. americanus) on 168 citrus plant material samples and 239 insect samples. Neither Ca. L. asiaticus nor Ca. L. americanus were detected in the host plants or insects vector, confirming the absence of the disease in the citrus-producing areas of Colombia.

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