Preeclampsia and ox-LDL modify the expression of autophagy markers in placenta and first trimester trophoblast cell line impairing trophoblast invasion and migration.

Placenta ◽  
2019 ◽  
Vol 83 ◽  
pp. e104-e105
Author(s):  
Lorena Carvajal ◽  
Claudette Cantin ◽  
Bárbara Fuenzalida ◽  
Susana Contreras-Duarte ◽  
Jaime Gutierrez ◽  
...  
Keyword(s):  
Cell Line ◽  
First Trimester ◽  
Trophoblast Cell ◽  
Trophoblast Invasion ◽  
Invasion And Migration ◽  
And Migration ◽  
Trophoblast Cell Line

scholarly journals The effect of IL-6 on the trophoblast cell line HTR-8/SVneo

2010 ◽  
Vol 62 (3) ◽  
pp. 531-538 ◽  
Author(s):  
Milica Jovanovic ◽  
Tamara Kovacevic ◽  
Ivana Stefanoska ◽  
Ljiljana Vicovac
Keyword(s):  
Cell Line ◽  
Normal Pregnancy ◽  
Blastocyst Stage ◽  
Uterine Wall ◽  
Trophoblast Cell ◽  
Trophoblast Invasion ◽  
Invasion And Migration ◽  
And Migration ◽  
Trophoblast Cell Line

Embryonic development up to the blastocyst stage, implantation into the uterine wall and the development of the functional placenta are steps crucial for the establishment of normal pregnancy. Specific cells of the placenta, the trophoblast cells, invade the uterine stroma and spiral arteries, adapting them to pregnancy. Interleukin-6 is present in the human endometrium during the receptive phase and early pregnancy. Trophoblasts also produce IL-6, which was found to stimulate trophoblast invasion and migration in vitro. Here we show that the activity of MMP-9 may contribute to the observed increased invasion. In addition, in the HTR-8/SVneo trophoblast cell line IL-6 increases cell proliferation. .

scholarly journals Human Chorionic Gonadotropin Stimulates Trophoblast Invasion through Extracellularly Regulated Kinase and AKT Signaling

Endocrinology ◽  
2007 ◽  
Vol 149 (3) ◽  
pp. 979-987 ◽  
Author(s):  
Johanna Prast ◽  
Leila Saleh ◽  
Heinrich Husslein ◽  
Stefan Sonderegger ◽  
Hanns Helmer ◽  
...  
Keyword(s):  
Western Blot ◽  
Chorionic Gonadotropin ◽  
First Trimester ◽  
Akt Signaling ◽  
Trophoblast Cell ◽  
Model Systems ◽  
Trophoblast Invasion ◽  
Invasion And Migration ◽  
Explant Cultures ◽  
And Migration

Chorionic gonadotropin (CG) is indispensable for human pregnancy because it controls implantation, decidualization, and placental development. However, its particular role in the differentiation process of invasive trophoblasts has not been fully unraveled. Here we demonstrate that the hormone promotes trophoblast invasion and migration in different trophoblast model systems. RT-PCR and Western blot analyses revealed expression of the LH/CG receptor in trophoblast cell lines and different trophoblast primary cultures. In vitro, CG increased migration and invasion of trophoblastic SGHPL-5 cells through uncoated and Matrigel-coated transwells, respectively. The hormone also increased migration of first-trimester villous explant cultures on collagen I. Proliferation of the trophoblast cell line and villous explant cultures measured by cumulative cell numbers and in situ 5-bromo-2′-deoxyuridine labeling, respectively, was unaffected by CG. Addition of the hormone activated ERK-1/2 and AKT in SGHPL-5 cells and pure, extravillous trophoblasts. Inhibition of MAPK kinase/ERK and phosphatidylinositide 3-kinase/AKT blocked phosphorylation of the kinases and attenuated CG-dependent invasion of SGHPL-5 cells. Similarly, the inhibitors decreased hormone-stimulated migration in villous explant cultures. Western blot analyses and gelatin zymography suggested that CG increased matrix metalloproteinase (MMP)-2 protein levels and activity in both culture systems. Inhibition of ERK or AKT diminished CG-induced MMP-2 expression. In summary, the data demonstrate that CG promotes trophoblast invasion and migration through activation of ERK and AKT signaling involving their downstream effector MMP-2. Because the increase of CG during the first trimester of pregnancy correlates with rising trophoblast motility, the hormone could be a critical regulator of the early invasion process.

Impact of human first trimester trophoblast cell line ACH-3P on the function of endothelial cells under different oxygen concentrations

Placenta ◽  
2013 ◽  
Vol 34 (9) ◽  
pp. A47
Author(s):  
Gregor Weiss ◽  
Ingrid Lang ◽  
Monika Siwetz ◽  
Berthold Huppertz ◽  
Gerit Moser
Keyword(s):  
Endothelial Cells ◽  
Cell Line ◽  
First Trimester ◽  
Trophoblast Cell ◽  
Trophoblast Cell Line ◽  
Oxygen Concentrations

Expression and Regulation of Vascular Endothelial Growth Factor in a First Trimester Trophoblast Cell Line

Placenta ◽  
2000 ◽  
Vol 21 (4) ◽  
pp. 320-324 ◽  
Author(s):  
I-B. Chung ◽  
F.D. Yelian ◽  
F.M. Zaher ◽  
B. Gonik ◽  
M.I. Evans ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Cell Line ◽  
First Trimester ◽  
Trophoblast Cell ◽  
Vascular Endothelial ◽  
Endothelial Growth Factor ◽  
Trophoblast Cell Line

scholarly journals The Cytokine Gene CXCL14 Restricts Human Trophoblast Cell Invasion by Suppressing Gelatinase Activity

Endocrinology ◽  
2009 ◽  
Vol 150 (12) ◽  
pp. 5596-5605 ◽  
Author(s):  
HaiBin Kuang ◽  
Qi Chen ◽  
Ying Zhang ◽  
Li Zhang ◽  
HongYing Peng ◽  
...  
Keyword(s):  
Trophoblast Cell ◽  
Trophoblast Invasion ◽  
Trophoblast Cells ◽  
Human Trophoblast ◽  
Gelatinase Activity ◽  
Invasion And Migration ◽  
Human Pregnancy ◽  
Decidual Cells ◽  
And Migration ◽  
Maternal Fetal Interface

Abstract Well-controlled trophoblast invasion into uterine decidua is a critical process for the normal development of placenta, which is tightly regulated by various factors produced within the trophoblast-endometrial microenvironment. CXCL14 is involved in tumor growth and metastasis, and its expression in placenta is temporally regulated during pregnancy. However, the role of CXCL14 in trophoblast function during human pregnancy is not clear. In this study, by using RT-PCR through human pregnancy, we found that CXCL14 was selectively expressed at early but not late pregnancy. Immunostaining revealed that CXCL14 proteins were strongly expressed in villous cytotrophoblasts and moderately in decidualized stromal cells but very weakly in syncytiotrophoblasts and extravillous trophoblasts. The effect of CXCL14 on trophoblast invasion were examined by using human villous explants cultured on Matrigel and further proved by invasion and migration assay of primary trophoblast cells and trophoblast cell line HTR-8/SVneo. Our data showed that CXCL14 significantly inhibited outgrowth of villous explant in vitro; this effect is due to suppression of trophoblast invasion and migration through regulating matrix metalloproteinases activities, whereas the trophoblast proliferation was not affected. Moreover, because a receptor for CXCL14 has not been identified, we performed further cell-specific CXCL14 binding activities with regard to different cell types within the maternal-fetal interface. Our data revealed that CXCL14 could specifically bind to trophoblast cells but not decidual cells from the maternal-fetal interface. These results suggest that CXCL14 plays an important role in regulating trophoblast invasion through an autocrine/paracrine manner during early pregnancy.

scholarly journals The Isolation and Characterization of a Novel Telomerase Immortalized First Trimester Trophoblast Cell Line, Swan 71

Placenta ◽  
2009 ◽  
Vol 30 (11) ◽  
pp. 939-948 ◽  
Author(s):  
S.L. Straszewski-Chavez ◽  
V.M. Abrahams ◽  
A.B. Alvero ◽  
P.B. Aldo ◽  
Y. Ma ◽  
...  
Keyword(s):  
Cell Line ◽  
First Trimester ◽  
Trophoblast Cell ◽  
Isolation And Characterization ◽  
Trophoblast Cell Line

Proliferative and Migration Activity of JEG-3 Trophoblast Cell Line in the Presence of Cytokines

2015 ◽  
Vol 159 (4) ◽  
pp. 550-556 ◽  
Author(s):  
D. I. Sokolov ◽  
K. N. Furaeva ◽  
O. I. Stepanova ◽  
S. A. Sel’kov
Keyword(s):  
Cell Line ◽  
Trophoblast Cell ◽  
Migration Activity ◽  
And Migration ◽  
Trophoblast Cell Line
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