On the oxygen transport of red blood cells in the feto-placental vasculature system of the mouse placenta

Placenta ◽  
2016 ◽  
Vol 45 ◽  
pp. 131
Author(s):  
Parisa Mirbod ◽  
Zhenxing Wu
Keyword(s):  
Red Blood Cells ◽  
Oxygen Transport ◽  
Blood Cells ◽  
Mouse Placenta

scholarly journals Red blood cells protect oxygen transport with adrenergic sodium-proton exchangers in hypoxic and hypercapnic white seabass

2021 ◽  
Author(s):  
Till S. Harter ◽  
Alexander M. Clifford ◽  
Martin Tresguerres
Keyword(s):  
Red Blood Cells ◽  
Oxygen Transport ◽  
Blood Cells ◽  
Subcellular Location ◽  
Ph Sensitive ◽  
Protective Effects ◽  
Adrenergic Stimulation ◽  
Low Oxygen ◽  
Binding Characteristics

AbstractWhite seabass (Atractoscion nobilis) are increasingly experiencing periods of low oxygen (O2; hypoxia) and high carbon dioxide (CO2, hypercapnia) due to climate change and eutrophication of the coastal waters of California. Haemoglobin (Hb) is the principal O2 carrier in the blood and in many teleost fishes Hb-O2 binding is compromised at low pH. However, Hb is contained within red blood cells (RBC) that, in some species, regulate intracellular pH with adrenergically-stimulated sodium-proton-exchangers (β-NHE). We hypothesised that white seabass have RBC β-NHEs that protect the blood O2-carrying capacity during hypoxia and hypercapnia. In a series of in vitro experiments, we determined the O2-binding characteristics of white seabass blood, the response of RBCs to adrenergic stimulation, and quantified the protective effect of β-NHE activity on Hb-O2 saturation during a hypercapnic acidosis in normoxia and hypoxia. White seabass had typical teleost Hb characteristics, with a moderate O2 affinity that was highly pH-sensitive. Functional, molecular and bioinformatic data confirmed that white seabass have RBC β-NHEs, and super-resolution imaging revealed, for the first time, the subcellular location of β-NHE protein in intracellular vesicles and on the RBC membrane. The activation of RBC β-NHEs increased Hb-O2 saturation by ∼8% in normoxia at 1% PCO2, and by ∼20% in hypoxia at arterial PCO2 (0.3%), but the protective effects decreased at higher PCO2. Combined, these data indicate that RBC β-NHE activity in white seabass can safeguard arterial O2 transport and the mechanism likely plays an important role in the fishes’ physiological response to environmental hypoxia and hypercapnia.Summary StatementWhite seabass have highly pH-sensitive haemoglobins, but their red blood cells can actively protect oxygen transport during hypoxia and hypercapnia, conditions that occur more frequently due to a changing climate.

CALCULATIONS OF OXYGEN TRANSPORT BY RED BLOOD CELLS AND HEMOGLOBIN SOLUTIONS IN CAPILLARIES

2002 ◽  
Vol 30 (3) ◽  
pp. 157-188 ◽  
Author(s):  
Arjun Vadapalli ◽  
Daniel Goldman ◽  
Aleksander S. Popel
Keyword(s):  
Red Blood Cells ◽  
Oxygen Transport ◽  
Blood Cells

scholarly journals Oxygen Transport during Ex Situ Machine Perfusion of Donor Livers Using Red Blood Cells or Artificial Oxygen Carriers

2020 ◽  
Vol 22 (1) ◽  
pp. 235
Author(s):  
Silke B. Bodewes ◽  
Otto B. van Leeuwen ◽  
Adam M. Thorne ◽  
Bianca Lascaris ◽  
Rinse Ubbink ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Oxygen Transport ◽  
Oxygen Delivery ◽  
Blood Cells ◽  
Marine Invertebrate ◽  
Oxygen Carrier ◽  
Ex Situ ◽  
Machine Perfusion ◽  
Oxygen Carriers ◽  
Advantages And Disadvantages

Oxygenated ex situ machine perfusion of donor livers is an alternative for static cold preservation that can be performed at temperatures from 0 °C to 37 °C. Organ metabolism depends on oxygen to produce adenosine triphosphate and temperatures below 37 °C reduce the metabolic rate and oxygen requirements. The transport and delivery of oxygen in machine perfusion are key determinants in preserving organ viability and cellular function. Oxygen delivery is more challenging than carbon dioxide removal, and oxygenation of the perfusion fluid is temperature dependent. The maximal oxygen content of water-based solutions is inversely related to the temperature, while cellular oxygen demand correlates positively with temperature. Machine perfusion above 20 °C will therefore require an oxygen carrier to enable sufficient oxygen delivery to the liver. Human red blood cells are the most physiological oxygen carriers. Alternative artificial oxygen transporters are hemoglobin-based oxygen carriers, perfluorocarbons, and an extracellular oxygen carrier derived from a marine invertebrate. We describe the principles of oxygen transport, delivery, and consumption in machine perfusion for donor livers using different oxygen carrier-based perfusion solutions and we discuss the properties, advantages, and disadvantages of these carriers and their use.

scholarly journals Cardiovascular System, Red Blood Cells, and Oxygen Transport in Microgravity

2016 ◽  
Author(s):  
Hanns-Christian Gunga ◽  
Victoria Weller von Ahlefeld ◽  
Hans-Joachim Appell Coriolano ◽  
Andreas Werner ◽  
Uwe Hoffmann
Keyword(s):  
Red Blood Cells ◽  
Cardiovascular System ◽  
Oxygen Transport ◽  
Blood Cells

Arterioles supply oxygen to capillaries by diffusion as well as by convection

1990 ◽  
Vol 258 (4) ◽  
pp. H1240-H1243 ◽  
Author(s):  
M. L. Ellsworth ◽  
R. N. Pittman
Keyword(s):  
Oxygen Saturation ◽  
Red Blood Cells ◽  
Oxygen Transport ◽  
Blood Cells ◽  
Oxygen Exchange ◽  
Cheek Pouch ◽  
Retractor Muscle ◽  
Hamster Cheek Pouch ◽  
Local Oxygen

In the early part of this century, August Krogh proposed a model of oxygen transport in capillaries that assumes that all oxygen is delivered to the capillaries by convection from small terminal arterioles and lost from these capillaries by diffusion. This model and its consequences have been used extensively to interpret whole organ oxygen transport data in terms of diffusion between capillaries and tissues and to relate changes in microvascular hemodynamics to alterations in oxygen transport. We evaluated the appropriateness of such extrapolation by measuring oxygen saturation at discrete locations along the lengths of individual capillaries in the hamster cheek pouch retractor muscle. Our results indicate that the amount of oxygen lost from individual capillaries can be markedly affected by the presence of larger microvessels that frequently cross the capillary path. These larger vessels act either as a diffusive supply of oxygen for the red blood cells within the capillary or as an additional sink for the oxygen depending on the direction of the oxygen tension gradient. This transfer of oxygen between larger microvessels and capillaries attenuates the importance of capillary hemodynamics in oxygen exchange. Therefore, conclusions about local oxygen exchange that utilize only hemodynamic data from whole organ or microvascular experiments and the Krogh model will generally be invalid and should be viewed with caution.

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