Enrofloxacin and toltrazuril are able to control Toxoplasma gondii infection in human trophoblast cells

Placenta ◽  
2015 ◽  
Vol 36 (4) ◽  
pp. 502 ◽  
Author(s):  
R.J. Silva ◽  
A.O. Gomes ◽  
J.R. Mineo ◽  
N.M. Silva ◽  
E.A.V. Ferro ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Trophoblast Cells ◽  
Human Trophoblast ◽  
Toxoplasma Gondii Infection ◽  
Human Trophoblast Cells

scholarly journals Biogenic Silver Nanoparticles Can Control Toxoplasma gondii Infection in Both Human Trophoblast Cells and Villous Explants

2021 ◽  
Vol 11 ◽  
Author(s):  
Idessania Nazareth Costa ◽  
Mayara Ribeiro ◽  
Priscila Silva Franco ◽  
Rafaela José da Silva ◽  
Thádia Evelyn de Araújo ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Toxoplasma Gondii ◽  
Chorionic Villus ◽  
Congenital Toxoplasmosis ◽  
Trophoblast Cells ◽  
Promising Alternative ◽  
Human Trophoblast ◽  
Biogenic Silver Nanoparticles ◽  
Bewo Cells ◽  
Toxoplasma Gondii Infection

The combination of sulfadiazine and pyrimethamine plus folinic acid is the conventional treatment for congenital toxoplasmosis. However, this classical treatment presents teratogenic effects and bone marrow suppression. In this sense, new therapeutic strategies are necessary to reduce these effects and improve the control of infection. In this context, biogenic silver nanoparticles (AgNp-Bio) appear as a promising alternative since they have antimicrobial, antiviral, and antiparasitic activity. The purpose of this study to investigate the action of AgNp-Bio in BeWo cells, HTR-8/SVneo cells and villous explants and its effects against Toxoplasma gondii infection. Both cells and villous explants were treated with different concentrations of AgNp-Bio or combination of sulfadiazine + pyrimethamine (SDZ + PYZ) in order to verify the viability. After, cells and villi were infected and treated with AgNp-Bio or SDZ + PYZ in different concentrations to ascertain the parasite proliferation and cytokine production profile. AgNp-Bio treatment did not reduce the cell viability and villous explants. Significant reduction was observed in parasite replication in both cells and villous explants treated with silver nanoparticles and classical treatment. The AgNp-Bio treatment increased of IL-4 and IL-10 by BeWo cells, while HTR8/SVneo cells produced macrophage migration inhibitory factor (MIF) and IL-4. In the presence of T. gondii, the treatment induced high levels of MIF production by BeWo cells and IL-6 by HTR8SV/neo. In villous explants, the AgNp-Bio treatment downregulated production of IL-4, IL-6, and IL-8 after infection. In conclusion, AgNp-Bio can decrease T. gondii infection in trophoblast cells and villous explants. Therefore, this treatment demonstrated the ability to reduce the T. gondii proliferation with induction of inflammatory mediators in the cells and independent of mediators in chorionic villus which we consider the use of AgNp-Bio promising in the treatment of toxoplasmosis in BeWo and HTR8/SVneo cell models and in chorionic villi.

scholarly journals Cyclooxygenase (COX)-2 modulates Toxoplasma gondii infection, immune response and lipid droplets formation in human trophoblast cells and villous explants

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Guilherme de Souza ◽  
Rafaela José Silva ◽  
Iliana Claudia Balga Milián ◽  
Alessandra Monteiro Rosini ◽  
Thádia Evelyn de Araújo ◽  
...  
Keyword(s):  
Immune Response ◽  
Toxoplasma Gondii ◽  
Inflammatory Cytokines ◽  
Lipid Droplets ◽  
Prostaglandin E ◽  
Trophoblast Cells ◽  
Human Trophoblast ◽  
Cox 2 ◽  
Cox 2 Inhibitors ◽  
Human Trophoblast Cells

AbstractCongenital toxoplasmosis is represented by the transplacental passage of Toxoplasma gondii from the mother to the fetus. Our studies demonstrated that T. gondii developed mechanisms to evade of the host immune response, such as cyclooxygenase (COX)-2 and prostaglandin E2 (PGE2) induction, and these mediators can be produced/stored in lipid droplets (LDs). The aim of this study was to evaluate the role of COX-2 and LDs during T. gondii infection in human trophoblast cells and villous explants. Our data demonstrated that COX-2 inhibitors decreased T. gondii replication in trophoblast cells and villous. In BeWo cells, the COX-2 inhibitors induced an increase of pro-inflammatory cytokines (IL-6 and MIF), and a decrease in anti-inflammatory cytokines (IL-4 and IL-10). In HTR-8/SVneo cells, the COX-2 inhibitors induced an increase of IL-6 and nitrite and decreased IL-4 and TGF-β1. In villous explants, the COX-2 inhibitors increased MIF and decreased TNF-α and IL-10. Furthermore, T. gondii induced an increase in LDs in BeWo and HTR-8/SVneo, but COX-2 inhibitors reduced LDs in both cells type. We highlighted that COX-2 is a key factor to T. gondii proliferation in human trophoblast cells, since its inhibition induced a pro-inflammatory response capable of controlling parasitism and leading to a decrease in the availability of LDs, which are essentials for parasite growth.

STAT-3, SMAD-2 and STAT-1 differentially regulate Toxoplasma gondii intracellular proliferation in human trophoblast cells

Placenta ◽  
2015 ◽  
Vol 36 (4) ◽  
pp. 511
Author(s):  
B.F. Barbosa ◽  
J.B. Lopes-Maria ◽  
A.O. Gomes ◽  
M.B. Angeloni ◽  
A.S. Castro ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Trophoblast Cells ◽  
Human Trophoblast ◽  
Intracellular Proliferation ◽  
Human Trophoblast Cells

596: Determinants of invasion in human trophoblast cells

2007 ◽  
Vol 197 (6) ◽  
pp. S172
Author(s):  
Kathryn Drennan ◽  
Adrian Platts ◽  
Amelia Linneman ◽  
Graham Johnson ◽  
Stephen Krawetz
Keyword(s):  
Trophoblast Cells ◽  
Human Trophoblast ◽  
Human Trophoblast Cells

scholarly journals PPARα agonists clofibrate and gemfibrozil inhibit cell growth, down-regulate hCG and up-regulate progesterone secretions in immortalized human trophoblast cells

2004 ◽  
Vol 68 (2) ◽  
pp. 313-321 ◽  
Author(s):  
Fumie Hashimoto ◽  
Yoshinobu Oguchi ◽  
Mieko Morita ◽  
Kikumi Matsuoka ◽  
Satoru Takeda ◽  
...  
Keyword(s):  
Cell Growth ◽  
Trophoblast Cells ◽  
Human Trophoblast ◽  
Inhibit Cell Growth ◽  
Human Trophoblast Cells

Syncytin-2 Plays an Important Role in the Fusion of Human Trophoblast Cells

2009 ◽  
Vol 392 (2) ◽  
pp. 301-318 ◽  
Author(s):  
Amandine Vargas ◽  
Julie Moreau ◽  
Sébastien Landry ◽  
Frédérique LeBellego ◽  
Chirine Toufaily ◽  
...  
Keyword(s):  
Trophoblast Cells ◽  
Human Trophoblast ◽  
Human Trophoblast Cells
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