Evaluation of the placental environment with a new in vitro model of histocultures of early and term placentae: determination of cytokine and chemokine expression profiles

Placenta ◽  
2005 ◽  
Vol 26 (2-3) ◽  
pp. 262-267 ◽  
Author(s):  
A. Faye ◽  
S. Pornprasert ◽  
G. Dolcini ◽  
P. Ave ◽  
J. Taïeb ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Expression Profiles ◽  
Chemokine Expression ◽  
Vitro Model

52 An in vitro model for determination of the rate of resistance development in HIV

1993 ◽  
Vol 20 ◽  
pp. 68 ◽  
Author(s):  
L. Vrang ◽  
C. Rydergȧrd ◽  
C. Ȧhgren ◽  
J. Wahlberg ◽  
M. Uhlén ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Resistance Development ◽  
Vitro Model

Determination of Functional ABCG2 Activity and Assessment of Drug–ABCG2 Interactions in Dairy Animals Using a Novel MDCKII In Vitro Model

2013 ◽  
Vol 102 (2) ◽  
pp. 772-784 ◽  
Author(s):  
Louise Wassermann ◽  
Sandra Halwachs ◽  
Stefan Lindner ◽  
Kerstin U. Honscha ◽  
Walther Honscha
Keyword(s):  
In Vitro Model ◽  
Dairy Animals ◽  
Vitro Model

Clearance of Levofloxacin by an in Vitro Model of Continuous Venovenous Hemodialysis (CVVHD)

2007 ◽  
Vol 30 (10) ◽  
pp. 889-895 ◽  
Author(s):  
S. Siewert ◽  
B. Drewelow ◽  
S.C. Mueller
Keyword(s):  
In Vitro Model ◽  
Urea Clearance ◽  
Flow Rates ◽  
Dialysate Flow ◽  
Dialysate Flow Rate ◽  
Continuous Venovenous Hemodialysis ◽  
Vitro Model ◽  
Positive Correlations

Information about the elimination and the adequate dosing of levofloxacin during renal replacement therapy is scarce. The aim of this study was to characterize in vitro the elimination of levofloxacin during continuous venovenous hemodialysis (CVVHD) and to investigate whether the CVVHD clearances of creatinine and urea are correlated with the levofloxacin clearance in order to facilitate dosage adjustments. An in vitro model of CVVHD was established using five dialyzer membranes at varying dialysate flow rates applied in the clinical setting (8, 16, 25, 33 and 41 ml/min). Plasma and dialysate samples were drawn for determination of levofloxacin, creatinine and urea concentrations to evaluate clearances by CVVHD. During CVVHD, the clearance of levofloxacin varied between 9.02 and 33.30 ml/min, depending on the chosen setup. Positive correlations (p<0.001) were received for: dialysate flow rate (QD) and creatinine/urea clearances (R>0.93); QD and levofloxacin clearance (R 0.59–0.71); levofloxacin and creatinine clearance (R 0.69–0.75); and levofloxacin and urea clearance (R 0.56–0.75) as well. When dosing critically ill patients, therefore, extracorporeal as well as total clearance of levofloxacin should be considered.

Comparison of Two Different In Vivo Models and an In Vitro Model for Caloric Determination of Four Novel Fiber Ingredients

2013 ◽  
Vol 61 (50) ◽  
pp. 12374-12379 ◽  
Author(s):  
Sarah Cervantes-Pahm ◽  
Brenda K. Knapp ◽  
Beob G. Kim ◽  
Yanhong Liu ◽  
Carl M. Parsons ◽  
...  
Keyword(s):  
In Vitro Model ◽  
In Vivo Models ◽  
Vitro Model

Volume-rendered, three-dimensional echocardiographi determination of the size, shape, and position of atrial septal defects: Validation in an in vitro model

1996 ◽  
Vol 132 (2) ◽  
pp. 376-381 ◽  
Author(s):  
Giuseppina Magni ◽  
Qi-Ling Cao ◽  
Lissa Sugeng ◽  
Alain Delabays ◽  
Gerald Marx ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Three Dimensional ◽  
Septal Defects ◽  
Atrial Septal Defects ◽  
Vitro Model

scholarly journals The transcriptional landscape of the murine middle ear epithelium in vitro

2019 ◽  
Author(s):  
Apoorva Mulay ◽  
Md Miraj K Chowdhury ◽  
Cameron James ◽  
Lynne Bingle ◽  
Colin D Bingle
Keyword(s):  
Gene Expression ◽  
Middle Ear ◽  
In Vitro Model ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Inflammatory Cells ◽  
Differentially Expressed ◽  
Vitro Model ◽  
Transcriptional Landscape

AbstractOtitis media (OM) is the most common paediatric disease and leads to significant morbidity. Although understanding of underlying disease mechanisms is hampered by complex pathophysiology, it is clear that epithelial abnormalities underpin the disease. The mechanisms underpinning epithelial remodelling in OM remain unclear. We recently described a novel in vitro model of mouse middle ear epithelial cells (mMEECs) that undergoes mucociliary differentiation into the varied epithelial cell populations seen in the middle ear cavity. We now describe genome wide gene expression profiles of mMEECs as they undergo differentiation. We compared the gene expression profiles of original (uncultured) middle ear cells, confluent cultures of undifferentiated cells (day 0 of ALI) and cells that had been differentiated for 7 days at an ALI. >5000 genes were differentially expressed among the three groups of cells. Approximately 4000 genes were differentially expressed between the original cells and day 0 of ALI culture. The original cell population was shown to contain a mix of cell types, including contaminating inflammatory cells that were lost on culture. Approximately 500 genes were upregulated during ALI induced differentiation. These included some secretory genes and some enzymes but most were associated with the process of ciliogenesis. Our in vitro model of differentiated murine middle ear epithelium exhibits a transcriptional profile consistent with the mucociliary epithelium seen within the middle ear. Knowledge of the transcriptional landscape of this epithelium will provide a basis for understanding the phenotypic changes seen in murine models of OM.

scholarly journals An in Vitro Model of Differentiation of Memory B Cells Into Plasmablasts and Plasma Cells Including Detailed Phenotypic and Molecular Characterization.

Blood ◽  
2009 ◽  
Vol 114 (22) ◽  
pp. 1636-1636
Author(s):  
Michel Jourdan ◽  
Anouk Caraux ◽  
John De Vos ◽  
Geneviève Fiol ◽  
Marion Larroque ◽  
...  
Keyword(s):  
B Cells ◽  
In Vitro Model ◽  
Plasma Cells ◽  
Conflicts Of Interest ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Memory B Cells ◽  
Vitro Model ◽  
Intermediate Cells

Abstract Abstract 1636 Poster Board I-662 Human plasma cells (PCs) and their precursors play an essential role in humoral immune response, but are rare and difficult to harvest. We report here i) the generation of human syndecan-1+ and immunoglobulin secreting PCs starting from memory B cells (MBCs) in a 3-step- and 10-day (D) culture, including a 6-fold cell amplification. ii) We report the detailed phenotypic and Affymetrix gene expression profiles of these in vitro PCs as well as of intermediate cells - activated B cells (actBCs) and plasmablasts (PBs) - compared to MBCs and bone marrow PCs, which is accessible through an open web ATLAS (http://amazonia.transcriptome.eu/). iii) We show this B cell to PC differentiation to involve IRF4 and AICDA expression in D4 actBCs, decrease of PAX5 and BCL6 expressions and increase in PRDM1 and XBP1 expressions in D7 PBs and D10 PCs. It involves downregulation of genes controlled by Pax5, induction of genes controlled by Blimp-1 and XBP1 (unfold protein response). iv) The phenotype of D10 PCs resembles that of peripheral blood PCs detected after immunization of healthy donors. This in vitro model will facilitate further studies in PC biology. It will likewise be helpful to study plasma-cell dyscrasias, including Multiple Myeloma. Disclosures No relevant conflicts of interest to declare.

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