A cytochrome P450 monooxygenase responsible for the C-22 hydroxylation step in the Paris polyphylla steroidal saponin biosynthesis pathway

2018 ◽  
Vol 156 ◽  
pp. 116-123 ◽  
Author(s):  
Yan Yin ◽  
Linhui Gao ◽  
Xianan Zhang ◽  
Wei Gao
Keyword(s):  
Cytochrome P450 ◽  
Cytochrome P450 Monooxygenase ◽  
Steroidal Saponin ◽  
Biosynthesis Pathway ◽  
P450 Monooxygenase ◽  
Saponin Biosynthesis ◽  
Paris Polyphylla

scholarly journals Cytochrome P450 Monooxygenase CYP716A141 is a Unique β-Amyrin C-16β Oxidase Involved in Triterpenoid Saponin Biosynthesis in Platycodon grandiflorus

2017 ◽  
Vol 58 (5) ◽  
pp. 874-884 ◽  
Author(s):  
Keita Tamura ◽  
Yuga Teranishi ◽  
Shinya Ueda ◽  
Hideyuki Suzuki ◽  
Noriaki Kawano ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Cytochrome P450 Monooxygenase ◽  
Triterpenoid Saponin ◽  
P450 Monooxygenase ◽  
Saponin Biosynthesis

scholarly journals Cytochrome P450 Monooxygenase CYP716A141 is a Unique b-Amyrin C-16b Oxidase Involved in Triterpenoid Saponin Biosynthesis in Platycodon grandiflorus

2017 ◽  
Vol 58 (6) ◽  
pp. 1119-1119 ◽  
Author(s):  
Keita Tamura ◽  
Yuga Teranishi ◽  
Shinya Ueda ◽  
Hideyuki Suzuki ◽  
Noriaki Kawano ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Cytochrome P450 Monooxygenase ◽  
Triterpenoid Saponin ◽  
P450 Monooxygenase ◽  
Saponin Biosynthesis

Molecular evolution of a cytochrome P450 for the synthesis of potential antidepressant (2R,6R)-hydroxynorketamine

2021 ◽  
Author(s):  
Ansgar Bokel ◽  
Michael C. Hutter ◽  
Vlada B. Urlacher
Keyword(s):  
Cytochrome P450 ◽  
Molecular Evolution ◽  
Cytochrome P450 Monooxygenase ◽  
P450 Monooxygenase ◽  
Effective Synthesis

Engineered cytochrome P450 monooxygenase CYP154E1 enables the effective synthesis of the potential antidepressant (2R,6R)-hydroxynorketamine via N-demethylation and regio- and stereoselective hydroxylation of (R)-ketamine.

scholarly journals Cytochrome P450 Monooxygenase-Mediated Metabolic Utilization of Benzo[a]Pyrene by Aspergillus Species

mBio ◽  
2019 ◽  
Vol 10 (3) ◽  
Author(s):  
Erin M. Ostrem Loss ◽  
Mi-Kyung Lee ◽  
Ming-Yueh Wu ◽  
Julia Martien ◽  
Wanping Chen ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Environmental Pollutants ◽  
Energy Generation ◽  
Fungal Species ◽  
Metabolic Changes ◽  
Cytochrome P450 Monooxygenase ◽  
Cellular Growth ◽  
P450 Monooxygenase ◽  
Content Type ◽  
Fundamental Knowledge

ABSTRACT Soil-dwelling fungal species possess the versatile metabolic capability to degrade complex organic compounds that are toxic to humans, yet the mechanisms they employ remain largely unknown. Benzo[a]pyrene (BaP) is a pervasive carcinogenic contaminant, posing a significant concern for human health. Here, we report that several Aspergillus species are capable of degrading BaP. Exposing Aspergillus nidulans cells to BaP results in transcriptomic and metabolic changes associated with cellular growth and energy generation, implying that the fungus utilizes BaP as a growth substrate. Importantly, we identify and characterize the conserved bapA gene encoding a cytochrome P450 monooxygenase that is necessary for the metabolic utilization of BaP in Aspergillus. We further demonstrate that the fungal NF-κB-type velvet regulators VeA and VelB are required for proper expression of bapA in response to nutrient limitation and BaP degradation in A. nidulans. Our study illuminates fundamental knowledge of fungal BaP metabolism and provides novel insights into enhancing bioremediation potential. IMPORTANCE We are increasingly exposed to environmental pollutants, including the carcinogen benzo[a]pyrene (BaP), which has prompted extensive research into human metabolism of toxicants. However, little is known about metabolic mechanisms employed by fungi that are able to use some toxic pollutants as the substrates for growth, leaving innocuous by-products. This study systemically demonstrates that a common soil-dwelling fungus is able to use benzo[a]pyrene as food, which results in expression and metabolic changes associated with growth and energy generation. Importantly, this study reveals key components of the metabolic utilization of BaP, notably a cytochrome P450 monooxygenase and the fungal NF-κB-type transcriptional regulators. Our study advances fundamental knowledge of fungal BaP metabolism and provides novel insight into designing and implementing enhanced bioremediation strategies.

Continuous cyclohexane oxidation to cyclohexanol using a novel cytochrome P450 monooxygenase fromAcidovoraxsp. CHX100 in recombinantP. taiwanensisVLB120 biofilms

2015 ◽  
Vol 113 (1) ◽  
pp. 52-61 ◽  
Author(s):  
Rohan Karande ◽  
Linde Debor ◽  
Diego Salamanca ◽  
Fabian Bogdahn ◽  
Karl-Heinrich Engesser ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Cytochrome P450 Monooxygenase ◽  
Cyclohexane Oxidation ◽  
P450 Monooxygenase

scholarly journals Importance of the cytochrome P450 monooxygenase CYP52 family for the sophorolipid-producing yeast Candida bombicola

2010 ◽  
Vol 10 (6) ◽  
pp. 791-791 ◽  
Author(s):  
Inge N.A. Van Bogaert ◽  
Marjan De Mey ◽  
Dirk Develter ◽  
Wim Soetaert ◽  
Erick J. Vandamme
Keyword(s):  
Cytochrome P450 ◽  
Cytochrome P450 Monooxygenase ◽  
Candida Bombicola ◽  
P450 Monooxygenase
Sign in / Sign up

Export Citation Format

Share Document