Discrimination of wild types and hybrids of Duboisia myoporoides and Duboisia leichhardtii at different growth stages using 1H NMR-based metabolite profiling and tropane alkaloids-targeted HPLC-MS analysis

2016 ◽  
Vol 131 ◽  
pp. 44-56 ◽  
Author(s):  
Sophie Friederike Ullrich ◽  
Nils J.H. Averesch ◽  
Leonardo Castellanos ◽  
Young Hae Choi ◽  
Andreas Rothauer ◽  
...  
Keyword(s):  
1H Nmr ◽  
Metabolite Profiling ◽  
Tropane Alkaloids ◽  
Growth Stages ◽  
Ms Analysis ◽  
Different Growth Stages

1H NMR based metabolite profiling with special focus on tropane alkaloids in Duboisia species

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
SF Ullrich ◽  
A Rothauer ◽  
O Kayser
Keyword(s):  
1H Nmr ◽  
Metabolite Profiling ◽  
Tropane Alkaloids ◽  
Special Focus

scholarly journals Evaluation of expression analysis of putrescine n-methyltransferase gene during different stages of growth in the medicinal plant Physalis divaricata (Solanaceae)

2017 ◽  
Vol 18 (4) ◽  
pp. 1430-1437
Author(s):  
ELHAM MOALLEM ◽  
ABDOLLAH GHASEMIPIRBALOUTI ◽  
TAHER NEJADSATTARI ◽  
ALIREZA IRANBAKHSH ◽  
IRAJ MEHREGAN
Keyword(s):  
Gene Expression ◽  
Medicinal Plant ◽  
Expression Analysis ◽  
Rna Extraction ◽  
Tropane Alkaloids ◽  
Expression Level ◽  
Growth Stages ◽  
Stages Of Growth ◽  
Methyltransferase Gene ◽  
Different Growth Stages

Moallem E, Ghasemipirbalouti A, Nejadsattari T, Iranbakhsh A, Mehregan I. 2017. Evaluation of expression analysis of putrescine n-methyltransferase gene during different stages of growth in the medicinal plant Physalis divaricata (Solanaceae). Biodiversitas 18: 1430-1437. Physalis divaricata (Solanaceae) is one of the most prevalent weeds in summer crops. Putrescine Nmethyltransferase (PMT) is a key enzyme in the biosynthesis of nicotine, tropane alkaloids atropine, scopolamine, cocaine, and calystegines. The present study set to compare PMT gene expression during different growth stages of Ph. divaricata using RT-qPCR assay. RNA extraction was performed from root and leaf samples of a total number of 40 individuals Ph. divaricata at different growth stages (late vegetative and fruiting stages) collected from southwestern Iran. RT-qPCR of cDNA reversely synthesized from RNA was carried out using SYBR®Premix Ex TaqTM II kit. PMT gene expression levels were analyzed using ΔΔCT method. The results showed that expression level of PMT in late vegetative stage samples was significantly higher compared to fruiting stage samples. The expression level of PMT similarly changed in root and leaf samples. Direct visualization of alkaloids in different tissues using Wagner histochemical tests showed more concentrations of alkaloids in leaf idioblasts and root stele.

Cytokinins from terminal buds of Euphoria longana during different growth stages

1997 ◽  
Vol 99 (1) ◽  
pp. 185-189
Author(s):  
Wen-Shaw Chen ◽  
Kuang-Liang Huang ◽  
Hsiao-Ching Yu
Keyword(s):  
Growth Stages ◽  
Terminal Buds ◽  
Different Growth Stages

Effects of Meteorological Factors at Different Growth Stages on Yield Traits of Maize (Zea maysL.) in Heilonggang Basin

2013 ◽  
Vol 39 (5) ◽  
pp. 919 ◽  
Author(s):  
Bo MING ◽  
Jin-Cheng ZHU ◽  
Hong-Bin TAO ◽  
Li-Na XU ◽  
Bu-Qing GUO ◽  
...  
Keyword(s):  
Meteorological Factors ◽  
Growth Stages ◽  
Yield Traits ◽  
Different Growth Stages
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