Carotenoid synthesis and phytoene synthase activity during mating of Blakeslea trispora

2012 ◽  
Vol 76 ◽  
pp. 40-45 ◽  
Author(s):  
Jürgen Breitenbach ◽  
Paul D. Fraser ◽  
Gerhard Sandmann
Keyword(s):  
Phytoene Synthase ◽  
Blakeslea Trispora ◽  
Carotenoid Synthesis

scholarly journals Expression of ZjPSY, A Phytoene Synthase Gene from Zoysia Japonica Leads to Leaf Yellowing and Plant Dwarfing in Arabidopsis Thaliana

2021 ◽  
Author(s):  
Di Dong ◽  
Ke Teng ◽  
Penghui Tan ◽  
Zhuocheng Liu ◽  
Zhuoxiong Yang ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Carotenoid Biosynthesis ◽  
Regulatory Elements ◽  
Phytoene Synthase ◽  
Grass Species ◽  
Zoysia Japonica ◽  
Altered Expression ◽  
Plant Expression Vector ◽  
Young Leaves ◽  
Carotenoid Synthesis

Abstract Phytoene synthase (Psy) is a key limiting enzyme in the carotenoid biosynthesis pathway by regulating phytoene synthesis. In this study, ZjPSY was isolated and identified from an important lawn grass species, Zoysia japonica. ZjPSY cDNA was 1230 bp in length, corresponding to 409 amino acids. ZjPSY showed higher expression in young leaves and were down-regulated after GA3, ABA, SA, and MeJA treatments, exhibited a sensitivity to hormones. By analysis of cis-regulatory elements in ZjPSY promoter region, ZjPSY exhibited be regulated of light and multiple hormones. To investigate the functions of ZjPSY, the plant expression vector was constructed to obtain transgenic Arabidopsis thaliana. Overexpression of ZjPSY protein led to carotenoid accumulation and altered expression of genes involved in carotenoid contents. ZjPSY expressing Arabidopsis thaliana exhibited yellowing and dwarfing phenotypes and contained more carotenoids than the wild type. Yeast two-hybrid screening identified a novel interacting partner of ZjPSY, ZjJ2 (DNAJ homologue 2), which encodes heat-shock protein 40 (HSP40). Taken together, this study suggests that ZjPSY plays an important role in carotenoid synthesis, leaf color development and hormone response in transgenic plants. These results broadened the understanding of carotenoid synthesis pathways and laid a foundation for the exploration and utilization of PSY gene.

Variability and Phylogeny of the Pepper Phytoene Synthase Paralogs PSY1 and PSY2 in Species of Various Capsicum Complexes

2020 ◽  
Vol 495 (1) ◽  
pp. 282-288
Author(s):  
A. V. Shchennikova ◽  
E. A. Dyachenko ◽  
G. I. Efremov ◽  
M. A. Filyushin ◽  
E. Z. Kochieva
Keyword(s):  
Phytoene Synthase

scholarly journals Effects of exogenous lipids and cold acclimation on lycopene production and fatty acid composition in Blakeslea trispora

AMB Express ◽  
2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Feng Lingran ◽  
Wang Qiang ◽  
Yu Xiaobin ◽  
Fred Kwame
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Neutral Lipids ◽  
Feedback Regulation ◽  
Total Content ◽  
Blakeslea Trispora ◽  
Plant Oils ◽  
Intracellular Lipids ◽  
Lycopene Production

Abstract Exogenous lipids serving as stimulators to improve lycopene production in Blakeslea trispora have been widely reported. However, the selection basis of exogenous lipids and their effects on intracellular lipids are not very clear. In this study, five plant oils with different fatty acid compositions were selected to investigate their effects on lycopene production, fatty acid composition and the desaturation degree of intracellular lipids. Among the oils, soybean oil, with a fatty acid composition similar to that of mycelium, exhibited the best stimulating effect on lycopene formation (improvement of 82.1%). The plant oils enhanced the total content of intracellular lipids and the desaturation degree of reserve lipids due to the alteration of fatty acid composition, especially in neutral lipids. Lycopene production was increased with the improved desaturation degree of intracellular lipids, which may be attributed to the enhancement of storage capacity for lycopene in storage lipid, thus reducing the feedback regulation of free lycopene. In addition, the increase of the desaturation degree of reserve lipids through temperature-changing fermentation also enhanced lycopene production. The present study could serve as a basis for a better understanding of the relationship between the fatty acid composition of reserve lipids and lycopene production.

scholarly journals Combined Production of Astaxanthin and β-Carotene in a New Strain of the Microalga Bracteacoccus aggregatus BM5/15 (IPPAS C-2045) Cultivated in Photobioreactor

Biology ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 643
Author(s):  
Konstantin Chekanov ◽  
Daniil Litvinov ◽  
Tatiana Fedorenko ◽  
Olga Chivkunova ◽  
Elena Lobakova
Keyword(s):  
Bubble Column ◽  
Biomass Accumulation ◽  
Natural Antioxidants ◽  
Gas Chromatography Mass Spectrometry ◽  
Stage Scheme ◽  
Green Microalga ◽  
Animal Feeding ◽  
Carotenoid Synthesis ◽  
Bubble Column Photobioreactor ◽  
Β Carotene

Carotenoids astaxanthin and β-carotene are widely used natural antioxidants. They are key components of functional food, cosmetics, drugs and animal feeding. They hold leader positions on the world carotenoid market. In current work, we characterize the new strain of the green microalga Bracteacoccus aggregatus BM5/15 and propose the method of its culturing in a bubble-column photobioreactor for simultaneous production of astaxanthin and β-carotene. Culture was monitored by light microscopy and pigment kinetics. Fatty acid profile was evaluated by tandem gas-chromatography–mass spectrometry. Pigments were obtained by the classical two-stage scheme of autotrophic cultivation. At the first, vegetative, stage biomass accumulation occurred. Maximum specific growth rate and culture productivity at this stage were 100–200 mg∙L−1∙day−1, and 0.33 day−1, respectively. At the second, inductive, stage carotenoid synthesis was promoted. Maximal carotenoid fraction in the biomass was 2.2–2.4%. Based on chromatography data, astaxanthin and β-carotene constituted 48 and 13% of total carotenoid mass, respectively. Possible pathways of astaxanthin synthesis are proposed based on carotenoid composition. Collectively, a new strain B. aggregatus BM5/15 is a potential biotechnological source of two natural antioxidants, astaxanthin and β-carotene. The results give the rise for further works on optimization of B. aggregatus cultivation on an industrial scale.

Ectopic expression of a fruit phytoene synthase from Citrus paradisi Macf. promotes abiotic stress tolerance in transgenic tobacco

2012 ◽  
Vol 39 (12) ◽  
pp. 10201-10209 ◽  
Author(s):  
Luciana C. Cidade ◽  
Tahise M. de Oliveira ◽  
Amanda F. S. Mendes ◽  
Amanda F. Macedo ◽  
Eny I. S. Floh ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Stress Tolerance ◽  
Transgenic Tobacco ◽  
Abiotic Stress Tolerance ◽  
Ectopic Expression ◽  
Phytoene Synthase ◽  
Citrus Paradisi
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