Autophosphorylation profiling of Arabidopsis protein kinases using the cell-free system

2011 ◽  
Vol 72 (10) ◽  
pp. 1136-1144 ◽  
Author(s):  
Keiichirou Nemoto ◽  
Takuya Seto ◽  
Hirotaka Takahashi ◽  
Akira Nozawa ◽  
Motoaki Seki ◽  
...  
Keyword(s):  
Protein Kinases ◽  
Free System ◽  
Cell Free System ◽  
Arabidopsis Protein

Genome-wide biochemical analysis of Arabidopsis protein phosphatase using a wheat cell-free system

FEBS Letters ◽  
2012 ◽  
Vol 586 (19) ◽  
pp. 3134-3141 ◽  
Author(s):  
Hirotaka Takahashi ◽  
Akihiko Ozawa ◽  
Keiichirou Nemoto ◽  
Akira Nozawa ◽  
Motoaki Seki ◽  
...  
Keyword(s):  
Protein Phosphatase ◽  
Biochemical Analysis ◽  
Free System ◽  
Cell Free System ◽  
Genome Wide ◽  
Arabidopsis Protein

scholarly journals Naphthalenesulphonamides block neutrophil superoxide production by intact cells and in a cell-free system: is myosin light chain kinase responsible for these effects?

1995 ◽  
Vol 311 (1) ◽  
pp. 81-87 ◽  
Author(s):  
P G Heyworth ◽  
R W Erickson ◽  
J Ding ◽  
J T Curnutte ◽  
J A Badwey
Keyword(s):  
Protein Kinase ◽  
Protein Kinases ◽  
Light Chain ◽  
Myosin Light Chain Kinase ◽  
Myosin Light Chain ◽  
Intact Cells ◽  
Free System ◽  
Cell Free System ◽  
A Cell

Selective antagonists of myosin light chain kinase (MLCK) [e.g. ML-7; 1-(5-iodonaphthalene-1-sulphonyl)-1H-hexahydro-1,4-diazepine hydrochloride] were found to inhibit superoxide (O2-) release from stimulated neutrophils. The concentrations of ML-7 that were inhibitory were substantially lower than those reported for a selective antagonist of protein kinase C [i.e. H-7; 1-(5-isoquinolinesulphonyl)-2-methylpiperazine dihydrochloride]. ML-7 also reduced the phosphorylation of the 47 kDa subunit of the NADPH-oxidase system (p47-phox) and blocked translocation of this protein to the Triton X-100-insoluble fraction in stimulated cells. Interestingly, ML-7 also inhibited O2- production in a cell-free system derived from neutrophils at concentrations similar to those that were effective in vivo. This cell-free system does not require ATP and is insensitive to all other inhibitors of protein kinases tested, including some highly effective against MLCK (i.e. staurosporine). Thus, the data suggest that ML-7 does not block O2- release by inhibiting a protein kinase but instead may interact directly with a subunit of the oxidase. The binding site for ML-7 may provide a valuable target for inhibiting the inflammatory properties of phagocytic leucocytes by naphthalenesulphonamides designed to lack activity against protein kinases.

Isolation of globin mRNA from spleen of anemic rabbits and its translation in wheat embryo cell-free system

1978 ◽  
Vol 43 (4) ◽  
pp. 1184-1189
Author(s):  
Ota Fuchs ◽  
Jitka Borová ◽  
Přemysl Poňka ◽  
Jan Neuwirt
Keyword(s):  
Embryo Cell ◽  
Globin Mrna ◽  
Free System ◽  
Wheat Embryo ◽  
Cell Free System

scholarly journals Effect of detergents on sterol synthesis in a cell-free system of yeast

1982 ◽  
Vol 23 (6) ◽  
pp. 803-810
Author(s):  
S Hata ◽  
T Nishino ◽  
N Ariga ◽  
H Katsuki
Keyword(s):  
Sterol Synthesis ◽  
Free System ◽  
Cell Free System ◽  
A Cell
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