Relative and accurate measurement of protein abundance using 15N stable isotope labeling in Arabidopsis (SILIA)

2011 ◽  
Vol 72 (10) ◽  
pp. 1028-1039 ◽  
Author(s):  
Guangyu Guo ◽  
Ning Li
Keyword(s):  
Stable Isotope ◽  
Isotope Labeling ◽  
Stable Isotope Labeling ◽  
Protein Abundance ◽  
15N Stable Isotope

scholarly journals 15N Stable Isotope Labeling PSTs in Alexandrium minutum for Application of PSTs as Biomarker

Toxins ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 211 ◽  
Author(s):  
Wancui Xie ◽  
Min Li ◽  
Lin Song ◽  
Rui Zhang ◽  
Xiaoqun Hu ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Isotope Labeling ◽  
Stable Isotope Labeling ◽  
15N Isotope ◽  
Atom Number ◽  
Alexandrium Minutum ◽  
15N Labeling ◽  
15N Stable Isotope ◽  
15N Abundance

The dinoflagellate Alexandrium minutum (A. minutum) which can produce paralyticshellfish toxins (PSTs) is often used as a model to study the migration, biotransformation,accumulation, and removal of PSTs. However, the mechanism is still unclear. To provide a new toolfor related studies, we tried to label PSTs metabolically with 15N stable isotope to obtain 15N-PSTsinstead of original 14N, which could be treated as biomarker on PSTs metabolism. We then culturedthe A. minutum AGY-H46 which produces toxins GTX1-4 in f/2 medium of different 15N/Pconcentrations. The 15N-PSTs’ toxicity and toxin profile were detected. Meanwhile, the 15N labelingabundance and 15N atom number of 15N-PSTs were identified. The 14N of PSTs produced by A.minutum can be successfully replaced by 15N, and the f/2 medium of standard 15N/P concentrationwas the best choice in terms of the species’ growth, PST profile, 15N labeling result and experimentcost. After many (>15) generations, the 15N abundance in PSTs extract reached 82.36%, and the 15Natom number introduced into GTX1-4 might be 4–6. This paper innovatively provided the initialevidence that 15N isotope application of labeling PSTs in A. minutum is feasible. The 15N-PSTs asbiomarker can be applied and provide further information on PSTs metabolism.

scholarly journals 15N Stable Isotope Labeling and Comparative Metabolomics Facilitates Genome Mining in Cultured Cyanobacteria

2020 ◽  
Vol 15 (3) ◽  
pp. 758-765 ◽  
Author(s):  
Daniel S. May ◽  
Camila M. Crnkovic ◽  
Aleksej Krunic ◽  
Tyler A. Wilson ◽  
James R. Fuchs ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Isotope Labeling ◽  
Genome Mining ◽  
Stable Isotope Labeling ◽  
Comparative Metabolomics ◽  
15N Stable Isotope

scholarly journals Tracing the fate of phosphorus fertilizer derived cadmium in soil-fertilizer-wheat systems using enriched stable isotope labeling

2021 ◽  
pp. 117314
Author(s):  
Christoph Bracher ◽  
Emmanuel Frossard ◽  
Moritz Bigalke ◽  
Martin Imseng ◽  
Jochen Mayer ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Isotope Labeling ◽  
Stable Isotope Labeling ◽  
Phosphorus Fertilizer ◽  
Soil Fertilizer
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