13CO2 as a universal metabolic tracer in isotopologue perturbation experiments

2007 ◽  
Vol 68 (16-18) ◽  
pp. 2273-2289 ◽  
Author(s):  
Werner Römisch-Margl ◽  
Nicholas Schramek ◽  
Tanja Radykewicz ◽  
Christian Ettenhuber ◽  
Eva Eylert ◽  
...  
Keyword(s):  
1984 ◽  
Vol 9 (2) ◽  
Author(s):  
Robert Dudczak ◽  
Kurt Kletter ◽  
Hans Frischauf ◽  
Udo Losert ◽  
Peter Angelberger ◽  
...  

2001 ◽  
Vol 97 (3) ◽  
pp. 271-273 ◽  
Author(s):  
N. Ishigure ◽  
T. Nakano ◽  
H. Enomoto
Keyword(s):  

Author(s):  
Jorge R. Barrio ◽  
Randy E. Keen ◽  
Diane C. Chugani ◽  
Gerald Bida ◽  
Nagichettiar Satyamurthy ◽  
...  

1983 ◽  
Vol 216 (1) ◽  
pp. 137-142 ◽  
Author(s):  
E H Goh

The feasibility of using exogenous [3H]desmosterol as a mass metabolic tracer for exogenous non-esterified cholesterol in hepatocytes is investigated with albumin-bound non-esterified cholesterol containing [3H]desmosterol and [14C] cholesterol tracers. The amounts of uptake and metabolism of exogenous cholesterol monitored by either tracer are the same. In addition, the conversion of [3H]desmosterol into [3H]cholesterol by the delta 24-sterol reductase in the microsomes can be used as an estimate for the mass transfer of exogenous cholesterol to the microsomes. The results obtained indicate that only a small fraction of exogenous cholesterol that was transferred to the microsomes was metabolized into bile acids and steryl esters. The technique of estimating the mass transfer of exogenous cholesterol to the microsomes with [3H]desmosterol may be of importance in investigations dealing with the effect of exogenous plasma cholesterol on changes in the physiological functions of the endoplasmic reticulum in the cells.


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