Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 35

2007 ◽  
Vol 68 (11) ◽  
pp. 1510-1520 ◽  
Author(s):  
Young Ock Ahn ◽  
Meiying Zheng ◽  
David R. Bevan ◽  
Asim Esen ◽  
Shin-Han Shiu ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Glycoside Hydrolase ◽  
Genomic Analysis ◽  
Glycoside Hydrolase Family ◽  
Functional Genomic ◽  
Functional Genomic Analysis ◽  
Hydrolase Family

Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 1

2004 ◽  
Vol 55 (3) ◽  
pp. 343-367 ◽  
Author(s):  
Zhiwei Xu ◽  
Luis Escamilla-Trevi�o ◽  
Lihui Zeng ◽  
Mallikarjun Lalgondar ◽  
David Bevan ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Glycoside Hydrolase ◽  
Genomic Analysis ◽  
Glycoside Hydrolase Family ◽  
Functional Genomic ◽  
Functional Genomic Analysis ◽  
Glycoside Hydrolase Family 1 ◽  
Hydrolase Family

Substrate specificity of glycoside hydrolase family 1 β-glucosidase AtBGlu42 from Arabidopsis thaliana and its molecular mechanism

2021 ◽  
Author(s):  
Shu Horikoshi ◽  
Wataru Saburi ◽  
Jian Yu ◽  
Hideyuki Matsuura ◽  
James R Ketudat Cairns ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Glycoside Hydrolase ◽  
Sequence Similarity ◽  
Glycoside Hydrolase Family ◽  
Its Sequence ◽  
Cell Wall Metabolism ◽  
Bioactive Substances ◽  
Chain Length Specificity ◽  
Glycoside Hydrolase Family 1 ◽  
Hydrolase Family

ABSTRACT Plants possess many glycoside hydrolase family 1 (GH1) β-glucosidases, which physiologically function in cell wall metabolism and activation of bioactive substances, but most remain uncharacterized. One GH1 isoenzyme AtBGlu42 in Arabidopsis thaliana has been identified to hydrolyze scopolin using the gene deficient plants, but no enzymatic properties were obtained. Its sequence similarity to another functionally characterized enzyme Os1BGlu4 in rice suggests that AtBGlu42 also acts on oligosaccharides. Here, we show that the recombinant AtBGlu42 possesses high kcat/Km not only on scopolin, but also on various β-glucosides, cellooligosaccharides, and laminarioligosaccharides. Of the cellooligosaccharides, cellotriose was the most preferred. The crystal structure, determined at 1.7 Å resolution, suggests that Arg342 gives unfavorable binding to cellooligosaccharides at subsite +3. The mutants R342Y and R342A showed the highest preference on cellotetraose or cellopentaose with increased affinities at subsite +3, indicating that the residues at this position have an important role for chain length specificity.

A functional genomic analysis of Arabidopsis thaliana PP2C clade D

PROTOPLASMA ◽  
2013 ◽  
Vol 251 (1) ◽  
pp. 265-271 ◽  
Author(s):  
Alejandro Tovar-Mendez ◽  
Ján A. Miernyk ◽  
Elizabeth Hoyos ◽  
Douglas D. Randall
Keyword(s):  
Arabidopsis Thaliana ◽  
Genomic Analysis ◽  
Functional Genomic ◽  
Functional Genomic Analysis

scholarly journals Functional Genomic Analysis of the AUXIN/INDOLE-3-ACETIC ACID Gene Family Members in Arabidopsis thaliana

2005 ◽  
Vol 17 (12) ◽  
pp. 3282-3300 ◽  
Author(s):  
Paul J. Overvoorde ◽  
Yoko Okushima ◽  
José M. Alonso ◽  
April Chan ◽  
Charlie Chang ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Acetic Acid ◽  
Gene Family ◽  
Family Members ◽  
Genomic Analysis ◽  
Functional Genomic ◽  
Functional Genomic Analysis ◽  
Indole 3 Acetic Acid
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