Diterpene resin acid biosynthesis in loblolly pine (Pinus taeda): Functional characterization of abietadiene/levopimaradiene synthase (PtTPS-LAS) cDNA and subcellular targeting of PtTPS-LAS and abietadienol/abietadienal oxidase (PtAO, CYP720B1)

2006 ◽  
Vol 67 (15) ◽  
pp. 1572-1578 ◽  
Author(s):  
Dae-Kyun Ro ◽  
Jörg Bohlmann
Keyword(s):  
Pinus Taeda ◽  
Loblolly Pine ◽  
Resin Acid ◽  
Functional Characterization ◽  
Subcellular Targeting ◽  
Acid Biosynthesis

Cytochrome P450 mono-oxygenases in conifer genomes: discovery of members of the terpenoid oxygenase superfamily in spruce and pine

2006 ◽  
Vol 34 (6) ◽  
pp. 1209-1214 ◽  
Author(s):  
B. Hamberger ◽  
J. Bohlmann
Keyword(s):  
Cytochrome P450 ◽  
Loblolly Pine ◽  
Large Scale ◽  
Expressed Sequence Tag ◽  
Resin Acid ◽  
Functional Characterization ◽  
Structural Diversity ◽  
Resin Acids ◽  
Full Length Sequence ◽  
Diterpene Resin Acids

Diterpene resin acids, together with monoterpenes and sesquiterpenes, are the most prominent defence chemicals in conifers. These compounds belong to the large group of structurally diverse terpenoids formed by enzymes known as terpenoid synthases. CYPs (cytochrome P450-dependent mono-oxygenases) can further increase the structural diversity of these terpenoids. While most terpenoids are characterized as specialized or secondary metabolites, some terpenoids, such as the phytohormones GA (gibberellic acid), BRs (brassinosteroids) and ABA (abscisic acid), have essential functions in plant growth and development. To date, very few CYP genes involved in conifer terpenoid metabolism have been functionally characterized and were limited to two systems, yew (Taxus) and loblolly pine (Pinus taeda). The characterized yew CYP genes are involved in taxol diterpene biosynthesis, while the only characterized pine terpenoid CYP gene is part of DRA (diterpene resin acid) biosynthesis. These CYPs from yew and pine are members of two apparently conifer-specific CYP families within the larger CYP85 clan, one of four plant CYP multifamily clans. Other CYP families within the CYP85 clan were characterized from a variety of angiosperms with functions in terpenoid phytohormone metabolism of GA, BR, and ABA. The recent development of EST (expressed sequence tag) and FLcDNA (where FL is full-length) sequence databases and cDNA collections for species of two conifers, spruce (Picea) and pine, allows for the discovery of new terpenoid CYPs in gymnosperms by means of large-scale sequence mining, phylogenetic analysis and functional characterization. Here, we present a snapshot of conifer CYP data mining, discovery of new conifer CYPs in all but one family within the CYP85 clan, and suggestions for their functional characterization. This paper will focus on the discovery of conifer CYPs associated with diterpene metabolism and CYP with possible functions in the formation of GA, BR, and ABA in conifers.

scholarly journals Functional characterization of amyrin synthase involved in ursolic acid biosynthesis in Catharanthus roseus leaf epidermis

2013 ◽  
Vol 91 ◽  
pp. 122-127 ◽  
Author(s):  
Fang Yu ◽  
Antje M.K. Thamm ◽  
Darwin Reed ◽  
Nemesio Villa-Ruano ◽  
Alfonso Lara Quesada ◽  
...  
Keyword(s):  
Ursolic Acid ◽  
Catharanthus Roseus ◽  
Functional Characterization ◽  
Leaf Epidermis ◽  
Acid Biosynthesis

Functional characterization of two p-coumaroyl ester 3′-hydroxylase genes from coffee tree: evidence of a candidate for chlorogenic acid biosynthesis

2007 ◽  
Vol 64 (1-2) ◽  
pp. 145-159 ◽  
Author(s):  
Venkataramaiah Mahesh ◽  
Rachel Million-Rousseau ◽  
Pascaline Ullmann ◽  
Nathalie Chabrillange ◽  
José Bustamante ◽  
...  
Keyword(s):  
Chlorogenic Acid ◽  
Functional Characterization ◽  
Acid Biosynthesis ◽  
Coffee Tree

scholarly journals Towards Elucidating Carnosic Acid Biosynthesis in Lamiaceae: Functional Characterization of the Three First Steps of the Pathway in Salvia fruticosa and Rosmarinus officinalis

PLoS ONE ◽  
2015 ◽  
Vol 10 (5) ◽  
pp. e0124106 ◽  
Author(s):  
Dragana Božić ◽  
Dimitra Papaefthimiou ◽  
Kathleen Brückner ◽  
Ric C. H. de Vos ◽  
Constantinos A. Tsoleridis ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Rosmarinus Officinalis ◽  
Carnosic Acid ◽  
Acid Biosynthesis ◽  
Salvia Fruticosa
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