Colorimetric DNA detection of transgenic plants using gold nanoparticles functionalized with L-shaped DNA probes

2016 ◽  
Vol 75 ◽  
pp. 188-195 ◽  
Author(s):  
Elham Nourisaeid ◽  
Amir Mousavi ◽  
Ayyoob Arpanaei
Keyword(s):  
Gold Nanoparticles ◽  
Transgenic Plants ◽  
Dna Detection ◽  
Dna Probes

SERS-based DNA detection in aqueous solutions using oligonucleotide-modified Ag nanoprisms and gold nanoparticles

2013 ◽  
Vol 405 (18) ◽  
pp. 6131-6136 ◽  
Author(s):  
Min Liu ◽  
Zhuyuan Wang ◽  
Shenfei Zong ◽  
Ruohu Zhang ◽  
Dan Zhu ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Aqueous Solutions ◽  
Dna Detection

Sensitive DNA detection by polymerase chain reaction with gold nanoparticles

2018 ◽  
Vol 1038 ◽  
pp. 105-111 ◽  
Author(s):  
Li Zou ◽  
Ruidi Shen ◽  
Liansheng Ling ◽  
Gongke Li
Keyword(s):  
Polymerase Chain Reaction ◽  
Gold Nanoparticles ◽  
Dna Detection ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Application of Gold Nanoparticles for Electrochemical DNA Biosensor

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Ahmed Mishaal Mohammed ◽  
Ruslinda A. Rahim ◽  
Ibraheem Jaleel Ibraheem ◽  
Foo Kai Loong ◽  
Hasrul Hisham ◽  
...  
Keyword(s):  
Thin Films ◽  
Gold Nanoparticles ◽  
Dna Hybridization ◽  
Dna Detection ◽  
Dna Biosensor ◽  
Base Line ◽  
Frequency Range ◽  
Dna Immobilization ◽  
Electrochemical Dna Biosensor ◽  
Chemically Modified

An electrochemical DNA biosensor was successfully fabricated by using (3-aminopropyl)triethoxysilane (APTES) as a linker molecule combined with the gold nanoparticles (GNPs) on thermally oxidized SiO2thin films. The SiO2thin films surface was chemically modified with a mixture of APTES and GNPs for DNA detection in different time periods of 30 min, 1 hour, 2 hours, and 4 hours, respectively. The DNA immobilization and hybridization were conducted by measuring the differences of the capacitance value within the frequency range of 1 Hz to 1 MHz. The capacitance values for DNA immobilization were 160 μF, 77.8 μF, 70 μF, and 64.6 μF, respectively, with the period of time from 30 min to 4 hours. Meanwhile the capacitance values for DNA hybridization were 44 μF, 54 μF, 55 μF, and 61.5 μF, respectively. The capacitance value of bare SiO2thin film was 0.42 μF, which was set as a base line for a reference in DNA detection. The differences of the capacitance value between the DNA immobilization and hybridization revealed that the modified SiO2thin films using APTES and GNPs were successfully developed for DNA detection.

A linear DNA probe as an alternative to a molecular beacon for improving the sensitivity of a homogenous fluorescence biosensing platform for DNA detection using target-primed rolling circle amplification

RSC Advances ◽  
2015 ◽  
Vol 5 (6) ◽  
pp. 4019-4025 ◽  
Author(s):  
Fulin Zhou ◽  
Baoxin Li ◽  
Jiyuan Ma
Keyword(s):  
Fluorescence Quenching ◽  
Molecular Beacon ◽  
Dna Detection ◽  
Rolling Circle Amplification ◽  
Dna Probes ◽  
Dna Probe ◽  
Rolling Circle ◽  
Hairpin Probe ◽  
Linear Dna ◽  
Signal Probe

Linear single-labeled DNA probes are used in this RCA-based fluorescence strategy for DNA detection, which could effectively avoid the fluorescence quenching between neighboring signal probes using hairpin probe as signal probe.

Gold nanoparticles functionalized poly(3,4-ethylenedioxythiophene) thin film for highly sensitive label free DNA detection

2013 ◽  
Vol 5 (3) ◽  
pp. 684-689 ◽  
Author(s):  
S. Radhakrishnan ◽  
C. Sumathi ◽  
V. Dharuman ◽  
J. Wilson
Keyword(s):  
Thin Film ◽  
Gold Nanoparticles ◽  
Dna Detection ◽  
Label Free ◽  
Free Dna ◽  
Highly Sensitive

scholarly journals Gold nanoparticles-assisted plasmonic enhancement for DNA detection on the graphene-based portable SPR sensor

2020 ◽  
Author(s):  
Briliant Adhi Prabowo ◽  
Agnes Purwidyantri ◽  
Bei Liu ◽  
Hsin-Chih Lai ◽  
Kou-Chen Liu
Keyword(s):  
Gold Nanoparticles ◽  
Dna Hybridization ◽  
Limit Of Detection ◽  
Dna Detection ◽  
Integrated Approach ◽  
Optical Simulation ◽  
Range Limit ◽  
Plasmonic Enhancement ◽  
Spr Sensor ◽  
The Impact

The impact of different gold nanoparticles (GNPs) structures on the plasmonic enhancement for DNA detection is investigated on a few-layer graphene (FLG) surface plasmon resonance (SPR) sensor. Two distinct structures of gold nanourchins (GNu) and gold nanorods (GNr) were used to bind the uniquely designed single-stranded probe DNA (ssDNA) of Mycobacterium tuberculosis complex (MTBC) DNA. The two types of GNPs-ssDNA mixture were adsorbed onto the FLGcoated SPR sensor through the π-π stacking force between the ssDNA and the graphene layer. In the presence of the complementary single-stranded DNA (cssDNA), the hybridization process took place and gradually removed the probes from the graphene surface. From SPR sensor preparation, the annealing process of the Au layer of the SPR sensor effectively enhanced the FLG coverage leading to a higher load of the probe DNA onto the sensing interface. The FLG was shown effective in providing a larger surface area for biomolecular capture due to its roughness. Carried out in the DNA hybridization study with SPR sensor, GNu, with its rough and spikey structures, significantly reinforced the overall DNA hybridization signal than the GNr with smooth superficies, especially in capturing the probe DNA. The DNA hybridization detection assisted by GNu reached the femtomolar range limit of detection (LoD). An optical simulation validated the extreme plasmonic field enhancement at the tip of the GNu spicules. The overall integrated approach of graphene-based SPR sensor and GNu-assisted DNA detection provided the proof-of-concept for the possibility for Tuberculosis disease screening using a low-cost and portable system potentially applied in remote or third world countries.

Sign in / Sign up

Export Citation Format

Share Document