Are cats (Felis catus) from multi-cat households more stressed? Evidence from assessment of fecal glucocorticoid metabolite analysis

2013 ◽  
Vol 122 ◽  
pp. 72-75 ◽  
Author(s):  
D. Ramos ◽  
A. Reche-Junior ◽  
P.L. Fragoso ◽  
R. Palme ◽  
N.K. Yanasse ◽  
...  
Keyword(s):  
Felis Catus ◽  
Metabolite Analysis ◽  
Fecal Glucocorticoid

scholarly journals Validation of a Field Technique and Characterization of Fecal Glucocorticoid Metabolite Analysis in Wild Chimpanzees (Pan troglodytes)

2012 ◽  
Vol 75 (1) ◽  
pp. 57-64 ◽  
Author(s):  
CARSON M. MURRAY ◽  
MATTHEW R. HEINTZ ◽  
ELIZABETH V. LONSDORF ◽  
LISA A. PARR ◽  
RACHEL M. SANTYMIRE
Keyword(s):  
Pan Troglodytes ◽  
Metabolite Analysis ◽  
Field Technique ◽  
Fecal Glucocorticoid

scholarly journals Further engineering of R. toruloides for the production of terpenes from lignocellulosic biomass

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
James Kirby ◽  
Gina M. Geiselman ◽  
Junko Yaegashi ◽  
Joonhoon Kim ◽  
Xun Zhuang ◽  
...  
Keyword(s):  
Lignocellulosic Biomass ◽  
Copy Number ◽  
Mevalonate Pathway ◽  
Mevalonate Kinase ◽  
Metabolite Analysis ◽  
Lignocellulosic Hydrolysate ◽  
Microbial Conversion ◽  
Lignocellulosic Hydrolysates ◽  
Lignocellulosic Feedstocks ◽  
Fuels And Chemicals

Abstract Background Mitigation of climate change requires that new routes for the production of fuels and chemicals be as oil-independent as possible. The microbial conversion of lignocellulosic feedstocks into terpene-based biofuels and bioproducts represents one such route. This work builds upon previous demonstrations that the single-celled carotenogenic basidiomycete, Rhodosporidium toruloides, is a promising host for the production of terpenes from lignocellulosic hydrolysates. Results This study focuses on the optimization of production of the monoterpene 1,8-cineole and the sesquiterpene α-bisabolene in R. toruloides. The α-bisabolene titer attained in R. toruloides was found to be proportional to the copy number of the bisabolene synthase (BIS) expression cassette, which in turn influenced the expression level of several native mevalonate pathway genes. The addition of more copies of BIS under a stronger promoter resulted in production of α-bisabolene at 2.2 g/L from lignocellulosic hydrolysate in a 2-L fermenter. Production of 1,8-cineole was found to be limited by availability of the precursor geranylgeranyl pyrophosphate (GPP) and expression of an appropriate GPP synthase increased the monoterpene titer fourfold to 143 mg/L at bench scale. Targeted mevalonate pathway metabolite analysis suggested that 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase (HMGR), mevalonate kinase (MK) and phosphomevalonate kinase (PMK) may be pathway bottlenecks are were therefore selected as targets for overexpression. Expression of HMGR, MK, and PMK orthologs and growth in an optimized lignocellulosic hydrolysate medium increased the 1,8-cineole titer an additional tenfold to 1.4 g/L. Expression of the same mevalonate pathway genes did not have as large an impact on α-bisabolene production, although the final titer was higher at 2.6 g/L. Furthermore, mevalonate pathway intermediates accumulated in the mevalonate-engineered strains, suggesting room for further improvement. Conclusions This work brings R. toruloides closer to being able to make industrially relevant quantities of terpene from lignocellulosic biomass.

Ketamine for Refractory Chronic Migraine: an Observational Pilot Study and Metabolite Analysis

2021 ◽  
Author(s):  
Eric S. Schwenk ◽  
Marc C. Torjman ◽  
Ruin Moaddel ◽  
Jacqueline Lovett ◽  
Daniel Katz ◽  
...  
Keyword(s):  
Pilot Study ◽  
Chronic Migraine ◽  
Metabolite Analysis ◽  
Refractory Chronic Migraine
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