Lack of effects of clomipramine on Fos and NADPH-diaphorase double-staining in the periaqueductal gray after exposure to an innate fear stimulus

2008 ◽  
Vol 94 (3) ◽  
pp. 316-321 ◽  
Author(s):  
Fabrício A. Moreira ◽  
Francisco S. Guimarães
Keyword(s):  
Periaqueductal Gray ◽  
Nadph Diaphorase ◽  
Double Staining ◽  
Fear Stimulus ◽  
Innate Fear

P.1.h.020 Independent periaqueductal gray and hypothalamic circuits for flight response and oriented behaviours elicited by innate fear

2014 ◽  
Vol 24 ◽  
pp. S281-S282
Author(s):  
F. Ullah ◽  
T. Dos Anjos-Garcia ◽  
R. De Oliveira ◽  
I.R. Santos ◽  
N.C. Coimbra
Keyword(s):  
Periaqueductal Gray ◽  
Flight Response ◽  
Innate Fear

The rostrodorsal periaqueductal gray influences both innate fear responses and acquisition of fear memory in animals exposed to a live predator

2019 ◽  
Vol 224 (4) ◽  
pp. 1537-1551 ◽  
Author(s):  
Rodrigo de Andrade Rufino ◽  
Sandra Regina Mota-Ortiz ◽  
Miguel Antonio Xavier De Lima ◽  
Marcus Vinicius C. Baldo ◽  
Newton Sabino Canteras
Keyword(s):  
Fear Memory ◽  
Periaqueductal Gray ◽  
Innate Fear

T17. The Role of mPFC in Escape From an Innate Fear Stimulus

2018 ◽  
Vol 83 (9) ◽  
pp. S135
Author(s):  
Alfred Kaye ◽  
Alex Kwan
Keyword(s):  
Fear Stimulus ◽  
Innate Fear

Induction of NADPH-diaphorase activity in the rat periaqueductal gray matter after nociceptive visceral stimulation

1998 ◽  
Vol 793 (1-2) ◽  
pp. 333-336 ◽  
Author(s):  
Luigi Rodella ◽  
Rita Rezzani ◽  
Cristina Agostini ◽  
Rossella Bianchi
Keyword(s):  
Gray Matter ◽  
Periaqueductal Gray ◽  
Nadph Diaphorase ◽  
Periaqueductal Gray Matter ◽  
Diaphorase Activity

Three-Dimensional Morphology of c-Kit–Positive Cellular Network and Nitrergic Innervation in the Human Gut

2001 ◽  
Vol 125 (7) ◽  
pp. 899-904 ◽  
Author(s):  
Laszlo Nemeth ◽  
Prem Puri
Keyword(s):  
Nitric Oxide ◽  
Cellular Network ◽  
Laser Scanning ◽  
Muscle Fibers ◽  
Circular Muscle ◽  
Nadph Diaphorase ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Double Staining ◽  
Whole Mount

Abstract Context.—c-Kit–positive interstitial cells of Cajal (ICC) appear to play a key role in the normal motility function and development of intestine. Nitric oxide is considered to be the most important messenger of inhibitory nonadrenergic, noncholinergic nerves in the enteric nervous system. Objectives.—The aims of this study were to examine the distribution of nitrergic innervation and ICCs in normal human bowel and to demonstrate interconnections between ICCs and nitrergic nerves and smooth muscle fibers using histochemical and immunohistochemical double-staining methods with a whole-mount preparation technique and confocal laser scanning microscopy. Methods.—Full-thickness small and large bowel specimens were obtained at autopsy from 18 children who died of nongastrointestinal diseases. A whole-mount preparation was performed for all specimens, and double staining was carried out with nicotinamide adenine dinucleotide phosphate (reduced form, NADPH)-diaphorase and c-Kit immunohistochemistry. Double immunofluorohistochemistry with neuronal nitric oxide synthase and c-Kit using confocal laser scanning microscopy was also performed in all specimens. Results.—The whole-mount preparation facilitated 3-dimensional visualization of the meshlike network of NADPH-diaphorase–positive nerve fibers in the myenteric plexus surrounded by a reticular network of c-Kit–positive ICCs. The dense c-Kit–positive cellular network located between longitudinal and circular muscle layers and at the innermost part of circular muscle layer intermingled with the myenteric plexus. Short, fine processes of ICCs made connections with the muscle fibers and c-Kit–positive cells. Conclusions.—The development of double–NADPH-diaphorase histochemistry and c-Kit immunohistochemistry staining technique in a whole-mount preparation provides an easy and useful method for investigating the association between c-Kit–positive cellular network and nitrergic neuronal network in the human bowel wall. The characteristic profiles of the c-Kit–positive cellular network and nitrergic neuronal network and their relationship with the smooth muscle fibers provide a morphologic basis for investigating intestinal motility disorders.

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