The persistence length and length per base of single-stranded DNA obtained from fluorescence correlation spectroscopy measurements using mean field theory

2013 ◽  
Vol 392 (5) ◽  
pp. 1072-1079 ◽  
Author(s):  
Qingjia Chi ◽  
Guixue Wang ◽  
Jiahuan Jiang
Keyword(s):  
Field Theory ◽  
Fluorescence Correlation Spectroscopy ◽  
Mean Field Theory ◽  
Persistence Length ◽  
Mean Field ◽  
Correlation Spectroscopy ◽  
Single Stranded Dna ◽  
Fluorescence Correlation

A BEAD-SPRING MODEL AND MEAN FIELD THEORY BASED RE-CALCULATION REVEALS UNCERTAINTY OF ROUSE-TYPE DNA DYNAMICS IN DILUTE SOLUTION

2012 ◽  
Vol 24 (04) ◽  
pp. 355-364 ◽  
Author(s):  
Qingjia Chi ◽  
Jiahuan Jiang
Keyword(s):  
Field Theory ◽  
Experimental Data ◽  
Mean Field Theory ◽  
Mean Field ◽  
Mean Square Displacement ◽  
Correlation Spectroscopy ◽  
Polymer Dynamics ◽  
Dilute Solution ◽  
Spring Model ◽  
Rouse Theory

Double-stranded DNA (dsDNA) is one of the most used model polymers in studying polymer dynamics. Some recent studies with the experimental data via fluorescence correlation spectroscopy (FCS) proposed that the end-monomer dynamics of dsDNA in dilute solution should be fallen into Rouse-type at intermediate times. This viewpoint is inconsistent with the classical polymer dynamics, therefore arousing controversy. To have a further looking clearly at what else meaning could be revealed from the original data of the FCS measurements, we made a re-calculation by two methods: one is based on Lodge and Wu's model (LWM) modified from the classical bead-spring model, in which parameters used in modeling are needed to be adjusted; and another is a mean field theory (MFT) for semiflexible chain with no parameter fitting needed. In LWM, we find not so weak hydrodynamic interactions (HI) which is not expected in Rouse theory, and the scaling of mean square displacement (MSD) is between Rouse-type and Zimm-type. MFT can reproduce experimental data well at larger time scales, whereas also gives rather different picture in intermediate regime — a dynamical scaling between Rouse-like and Zimm-like rather than Rouse-like scaling is found, indicating there may be sample problems or limitations in the setup for the experiment.

Mean field theory of n-layer unbinding

1993 ◽  
Vol 3 (3) ◽  
pp. 385-393 ◽  
Author(s):  
W. Helfrich
Keyword(s):  
Field Theory ◽  
Mean Field Theory ◽  
Mean Field
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