scholarly journals Solvent viscosity dependence for enzymatic reactions

2008 ◽  
Vol 387 (22) ◽  
pp. 5483-5497 ◽  
Author(s):  
A.E. Sitnitsky
Keyword(s):  
Enzymatic Reactions ◽  
Solvent Viscosity ◽  
Viscosity Dependence

Development of 96 Multiple Injection-GC-MS Technique and Its Application in Protein Engineering of Natural and Non-Natural Enzymatic Reactions

2019 ◽  
Author(s):  
Anja Knorrscheidt ◽  
Pascal Püllmann ◽  
Eugen Schell ◽  
Dominik Homann ◽  
Erik Freier ◽  
...  
Keyword(s):  
Microtiter Plate ◽  
Axial Ligand ◽  
Cell System ◽  
Enzymatic Reactions ◽  
The Novel ◽  
Internal Standards ◽  
E Coli ◽  
Enzyme Screening ◽  
Plate Analysis ◽  
Microtiter Plate Analysis

Directed evolution requires the screening of enzyme libraries in biological matrices. Available assays are mostly substrate or enzyme specific. Chromatographic techniques like LC and GC overcome this limitation, but require long analysis times. The herein developed multiple injections in a single experimental run (MISER) using GC coupled to MS allows the injection of samples every 33 s resulting in 96-well microtiter plate analysis within 50 min. This technique is implementable in any GC-MS system with autosampling. Since the GC-MS is far less prone to ion suppression than LCMS, no chromatographic separation is required. This allows the utilisation of an internal standards and the detection of main and side-product. To prove the feasibility of the system in enzyme screening, two libraries were assessed: i) YfeX library in an E. coli whole cell system for the carbene-transfer reaction on indole revealing the novel axial ligand tryptophan, ii) a library of 616 chimeras of fungal unspecific peroxygenase (UPO) in S. cerevisiae supernatant for hydroxylation of tetralin resulting in novel constructs. The data quality and representation are automatically assessed by a new R-script.

Modular Simulation to Determine the Optimal Operating Policy of a Batch Reactor for the Enzymatic Fructose Reduction to Mannitol with the in situ Continuous Enzymatic Regeneration of the NAD Cofactor

2017 ◽  
Vol 68 (9) ◽  
pp. 2196-2203 ◽  
Author(s):  
Mara Crisan ◽  
Gheorghe Maria
Keyword(s):  
Batch Reactor ◽  
Production Capacity ◽  
Optimization Procedure ◽  
Enzymatic Reactions ◽  
Mannitol Dehydrogenase ◽  
Optimal Operating ◽  
Operating Policy ◽  
Enzymatic Reduction ◽  
Optimal Operating Policy

Novel coupled enzymatic systems reported important applications in the industrial bio-catalysis. Multi-enzymatic reactions can successfully replace complex chemical syntheses, using milder reaction conditions, and generating less waste. For such systems acting simultaneously, the model-based engineering calculations (design, reactor operation optimization) are difficult tasks, because they must account for interacting reactions, differences in enzymes optimal activity domains and deactivation kinetics. The determination of the optimal operating mode (enzyme ratios, enzyme feeding policy, temperature, pH) often turns into a difficult multi-objective optimization problem with multiple constraints to be solved for every particular system. The paper focuses on applying a modular screening procedure that can identify the optimal operating policy of an enzymatic reactor, which minimizes the enzyme consumption, given the process kinetic model, and an imposed production capacity. Following an optimization procedure, the process effectiveness is evaluated in a systematic approach, by including simple batch reactor (BR), batch with intermittent addition of the key-enzyme following certain optimal policies (BRP). Exemplification is made for the case of the enzymatic reduction of D-fructose to mannitol by using suspended MDH (mannitol dehydrogenase) and NADH (Nicotinamide adenine dinucleotide) cofactor, with the in-situ continuous regeneration of the cofactor by the expense of formate degradation in the presence of suspended FDH (Formate dehydrogenase).

Stationary fluid convection modes with a Gaussian viscosity dependence of temterature

2016 ◽  
Vol 11 (2) ◽  
pp. 218-225
Author(s):  
V.S. Kuleshov
Keyword(s):  
Computer Code ◽  
Boussinesq Approximation ◽  
Simple Type ◽  
Transfer Coefficients ◽  
Convective Flows ◽  
Heat Transfer Coefficients ◽  
Fluid Convection ◽  
Flat Cell ◽  
Volume Method ◽  
Viscosity Dependence

The results of a numerical modeling of thermo-gravitational convection of abnormally thermo-viscous fluid in a closed square cavity with two vertical adiabatic walls and two horizontal isothermal walls are presented. A model Newtonian liquid for which the dependence of viscosity on temperature is described by a bell function (Gaussian curve) is considered. The natural convection of inhomogeneous liquid is described by the closed mathematical model based on the continuous mechanics equations written in Oberbeck-Boussinesq approximation, where the fluid density is a linear function of temperature. To simulate the fluid flow dynamics, the modified computer code based on the implicit finite volume method and SIMPLE-type algorithm with the second-order temporal accuracy is realized using multiprocessor technology. The effect of the viscosity abnormality on stationary modes of convective flows are studied, the integral heat transfer coefficients in a flat cell are calculated.

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