In vitro decreases of the fibrinolytic potential of cultured human fibrosarcoma cell line, HT1080, by Nigella sativa oil

Phytomedicine ◽  
2005 ◽  
Vol 12 (1-2) ◽  
pp. 100-107 ◽  
Author(s):  
E.M. Awad
Keyword(s):  
Cell Line ◽  
Nigella Sativa ◽  
Fibrosarcoma Cell ◽  
Nigella Sativa Oil ◽  
Fibrinolytic Potential

A novel rat fibrosarcoma cell line from transformed bone marrow-derived mesenchymal stem cells with maintained in vitro and in vivo stemness properties

2017 ◽  
Vol 352 (2) ◽  
pp. 218-224 ◽  
Author(s):  
Meng-Yu Wang ◽  
Janne Nestvold ◽  
Øystein Rekdal ◽  
Gunnar Kvalheim ◽  
Øystein Fodstad
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Cell Line ◽  
Fibrosarcoma Cell

scholarly journals Combination Therapy of a Host Defense-Like Lytic Peptide and Continuous Low-Dose Doxorubicin Inhibits Tumor Growth in a Syngeneic Immunocompetent Murine Fibrosarcoma (BFS-1) Model

2020 ◽  
Author(s):  
Jan Philip Suppelna ◽  
Kamran Harati ◽  
Andrea Rittig ◽  
Ingo Stricker ◽  
Markus Lehnhardt ◽  
...  
Keyword(s):  
Mouse Model ◽  
Tumor Growth ◽  
Cell Line ◽  
Host Defense ◽  
Low Dose ◽  
Sarcoma Cell ◽  
Fibrosarcoma Cell ◽  
Murine Fibrosarcoma

Abstract Background: The concept of a multimodality therapy in the treatment of soft tissue sarcomas (STS) has been discussed with controversy. Surgical resection with clear margins and radiation therapy remain gold standard in STS therapy. It is still questionable whether a systemic therapy with chemotherapeutics has a positive impact on the overall survival rate especially in early stages of disease, because the therapeutic effect in the treatment of STS is limited by its toxicities and its low responding rates. Treatment options are rare. As a result the search for combination therapies by using low dose approaches is of high importance. Recent studies showed the therapeutic efficiency of a designer host defense-like lytic D,L- amino acid peptide [D]-K 3 H 3 L 9 . Therefore we tested a combination of this peptide with Doxorubicin on two different sarcoma cell lines in vitro and also in a syngeneic immunocompetent murine fibrosarcoma mouse model. Methods: In vitro the human synovial sarcoma cell line SW 982 and the murine fibrosarcoma cell line BFS-1 were exposed to the oncolytic peptide [D]-K 3 H 3 L 9 , to the Anthracycline Doxorubicin and to both agents simultaneously. In vivo the murine fibrosarcoma cell line BFS-1 was injected subcutaneously into the syngeneic mice. When the tumors engrafted the oncolytic designer peptide [D]-K 3 H 3 L 9 , Doxorubicin or a combination of both was administered thrice a week for a three weeks’ follow-up. Results: The combination treatment approach using an oncolytic designer host defense peptide and Doxorubicin inhibited the in vitro sarcoma cell proliferation significantly. The single therapies, either with local intratumoral application of [D]-K 3 H 3 L 9 or with intraperitoneal application of Doxorubicin in the syngeneic mouse model, inhibited at least the tumor progression. The combination of both substances revealed a significant inhibition of tumor growth and weight. Conclusion: The in vivo low dose combination schedule inhibited the tumor growth significantly. Histological analyses of the tumor sections revealed an antiproliferative and antiangiogenic effect. So, these results demonstrate the effectiveness of combined low-dose application forms with designer host defense-like lytic peptides and chemotherapeutics.

scholarly journals Assessment of In vitro antibacterial activity and cytotoxicity effect of Nigella sativa oil

2016 ◽  
Vol 12 (47) ◽  
pp. 471 ◽  
Author(s):  
AyseRuveyda Ugur ◽  
HaticeTurk Dagi ◽  
Bahadir Ozturk ◽  
Gulsum Tekin ◽  
Duygu Findik
Keyword(s):  
Antibacterial Activity ◽  
Nigella Sativa ◽  
Nigella Sativa Oil ◽  
In Vitro Antibacterial Activity

In vitro Anti-atherothrombosis Activity of Nigella sativa Oil, Phytol and their Combinations

2019 ◽  
Vol 53 (4) ◽  
pp. 670-674
Author(s):  
Muhammad Torequl Islam ◽  
Shamim Molla ◽  
Apu Kumar Das ◽  
Farhana Zaman ◽  
Md. Roich Khan
Keyword(s):  
Nigella Sativa ◽  
Nigella Sativa Oil

scholarly journals IN VITRO IMMUNOSTIMULATION ACTIVITY OF NIGELLA SATIVA LINN. AND PSORALEA CORYLIFOLIA LINN. SEEDS USING A MURINE MACROPHAGE CELL LINE

2017 ◽  
Vol 10 (3) ◽  
pp. 329
Author(s):  
Saravanan Periasamy ◽  
Sheik Noor Mohamed M ◽  
Jaikumar K ◽  
Marimuthu S ◽  
John Wyson W ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cell Line ◽  
Nigella Sativa ◽  
Ethanol Extract ◽  
Immunomodulatory Activity ◽  
Psoralea Corylifolia ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Plant Seeds

ABSTRACTObjective: The aim of this study is to analyze the phytochemical constituents and the in vitro immunomodulatory potential of ethanol extract ofNigella sativa and Psoralea corylifolia seeds.Methods: Phytoconstituents in the ethanol extract of N. sativa and P. corylifolia seeds were analyzed using gas chromatography and mass spectrumand their immunomodulatory activity was investigated in vitro by cell proliferation assay with J774A.1 cell line.Results: Thirteen biochemical constituents have been identified with ethanol extract of N. sativa and nine from the P. corylifolia. The maximum relativeactivity of 138.77±0.44% cell proliferation was expressed at 25 μg/ml by N. sativa and 80.70±0.42% of proliferation by P. corylifolia at 25 μg/ml.Conclusion: The results suggest that the ethanol extract of the plant seeds have stimulating activity on macrophage cells and could be useful formodulating immune functions.Keywords: Immunomodulatory, Cell proliferation, Macrophage cell line, Nigella sativa, Psoralea corylifolia.

scholarly journals Nigella sativa oil inhibits proliferation and stimulates apoptosis of human lymphocytes in vitro

2021 ◽  
Author(s):  
Klaudia Ciesielska-Figlon ◽  
Katarzyna A. Lisowska ◽  
Anna Mikosik-Roczynska ◽  
Jacek M. Witkowski
Keyword(s):  
Nigella Sativa ◽  
Human Lymphocytes ◽  
Nigella Sativa Oil
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