scholarly journals Autoimmune therapeutic chloroquine lowers blood pressure and improves endothelial function in spontaneously hypertensive rats

2016 ◽  
Vol 113 ◽  
pp. 384-394 ◽  
Author(s):  
Cameron G. McCarthy ◽  
Camilla F. Wenceslau ◽  
Styliani Goulopoulou ◽  
Safia Ogbi ◽  
Takayuki Matsumoto ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Endothelial Function ◽  
Spontaneously Hypertensive Rats ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive

Abstract 261: Chloroquine, an Inhibitor of Endosomal Toll-like Receptors, Improves Blood Pressure and Endothelial Function in Spontaneously Hypertensive Rats

Hypertension ◽  
2014 ◽  
Vol 64 (suppl_1) ◽  
Author(s):  
Cameron G McCarthy ◽  
Camilla F Wenceslau ◽  
Styliani Goulopoulou ◽  
Safia Ogbi ◽  
Takayuki Matsumoto ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Endothelial Function ◽  
Innate Immune System ◽  
Spontaneously Hypertensive Rats ◽  
Innate Immune ◽  
Hypertensive Rats ◽  
Dnase Ii ◽  
Spontaneously Hypertensive ◽  
Immune System Activation ◽  
System Activation

HBPR 2014 Circulating mitochondrial DNA (mtDNA) is elevated in spontaneously hypertensive rats (SHR) and the enzyme responsible for its degradation, DNase II, has decreased activity in SHR tissues. Moreover, a synthetic mtDNA mimetic, specific for TLR 9, causes endothelial dysfunction and elevated blood pressure. Therefore, we sought to inhibit the contribution of mtDNA on innate immune system activation, via inhibition of Toll-like receptor (TLR)9, in SHR. We treated 12-15 week old SHR and Wistar-Kyoto rats (WKY) with chloroquine (CQ; 40mg/kg/day) or vehicle (veh; saline) for 21 days via intraperitoneal injection ( i.p. ). We hypothesized that CQ treatment would improve endothelial function and decrease blood pressure in SHR. Blood pressure was measured pre- and post-treatment via tail cuff and endothelial function was measured via mesenteric resistance artery (MRA) relaxation to acetylcholine (ACh; 10 -9 -10 -5 M) using a wire myograph. Treatment with CQ lowered post-systolic blood pressure in SHR (mmHg, Veh: 201±2 vs. CQ: 185±5 mmHg; p<0.05). No effects of treatment were observed on blood pressure in WKY (p>0.05). CQ abolished the contractile effect to high concentrations of ACh [AUC (%NE pre-contraction), Veh: 160±25 vs. CQ: 277±20; p<0.05] in MRA from SHR. However, no differences were observed in endothelium-independent relaxation to NO-donor sodium nitroprusside [E max (%NE pre-contraction), Veh: 97±12 vs. CQ: 102±26; p<0.05] in MRAs from SHR. Again, no effects of treatment were observed for endothelium-dependent or -independent relaxation in WKY (p>0.05). These data demonstrate the inhibition of endosomal TLRs, including TLR9, improves blood pressure and endothelial function in SHR. Inhibition of TLR9 abrogates the potentially deleterious contribution of increased mtDNA and impaired DNase II activity on innate immune system activation in hypertension.

Antihypertensive effects of oleuropein-enriched olive leaf extract in spontaneously hypertensive rats

2016 ◽  
Vol 7 (1) ◽  
pp. 584-593 ◽  
Author(s):  
M. Romero ◽  
M. Toral ◽  
M. Gómez-Guzmán ◽  
R. Jiménez ◽  
P. Galindo ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Endothelial Function ◽  
Spontaneously Hypertensive Rats ◽  
Leaf Extract ◽  
Olive Leaf ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Olive Leaf Extract ◽  
Inflammatory Status

The effects of chronic consumption of oleuropein-enriched (15% w/w) olive leaf extract (OLE) on blood pressure, endothelial function, and vascular oxidative and inflammatory status in spontaneously hypertensive rats (SHR) were evaluated.

scholarly journals Gene-Level Regulation of Acupuncture Therapy in Spontaneously Hypertensive Rats: A Whole Transcriptome Analysis

2019 ◽  
Vol 2019 ◽  
pp. 1-13 ◽  
Author(s):  
Si-Ming Ma ◽  
Jing-Wen Yang ◽  
Jian-Feng Tu ◽  
Na-Na Yang ◽  
Yu-Zheng Du ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Blood Pressure ◽  
Endothelial Function ◽  
Transcriptome Analysis ◽  
Stress Responses ◽  
Spontaneously Hypertensive Rats ◽  
Vascular Endothelial ◽  
Vascular Endothelial Function ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive

Hypertension is a global health problem. It has been reported that acupuncture at Taichong acupoints (LR3) decreases high blood pressure in spontaneously hypertensive rats. A transcriptome analysis can profile gene expression and its relationship with acupuncture. In this study, rats were treated with 2 weeks of acupuncture followed by regular recording of blood pressure (BP). The mRNA changes in the rostral ventrolateral medulla (RVLM) were evaluated to uncover the genetic mechanisms of acupuncture by using a whole transcript array (Affymetrix Rat Gene 1.0 ST array). BP measurements showed that acupuncture significantly decreased systolic blood pressure (SBP), mean arterial pressure (MAP), and heart rate (HR). In the bioinformatics results, 2371 differentially expressed genes (DEGs) were identified, where 83 DEGs were overlapped among Wistar-Kyoto rats (WKYs), spontaneously hypertensive rats (SHRs), and SHRs + acupuncture rats (SHRs+Acu). Gene ontology (GO) and pathway analysis revealed that 279 GO terms and 20 pathways with significant differences were related to oxidative stress, inflammation, and vascular endothelial function. In addition, coexpressed DEGs networks indicated that Cd4 and Il-33 might mediate the cascade of inflammation and oxidative stress responses, which could serve as a potential target of acupuncture treatment. In conclusion, our study demonstrated that acupuncture is a promising therapy for treating hypertension and could regulate multiple biological processes mainly involving oxidative stress, inflammation, and vascular endothelial function.

scholarly journals Dietary fish oil improves endothelial function and lowers blood pressure via suppression of sphingolipid-mediated contractions in spontaneously hypertensive rats

2014 ◽  
Vol 32 (5) ◽  
pp. 1050-1058 ◽  
Author(s):  
Lieke W.J. van den Elsen ◽  
Léon J.A. Spijkers ◽  
Rob F.P. van den Akker ◽  
Aggie M.H. van Winssen ◽  
Martin Balvers ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Endothelial Function ◽  
Fish Oil ◽  
Spontaneously Hypertensive Rats ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Dietary Fish

Treatment with an extract of Terminalia superba Engler & Diels decreases blood pressure and improves endothelial function in spontaneously hypertensive rats

2014 ◽  
Vol 151 (1) ◽  
pp. 372-379 ◽  
Author(s):  
Esther Ngo Lemba Tom ◽  
Corine Girard-Thernier ◽  
Hélène Martin ◽  
Théophile Dimo ◽  
Magalie Alvergnas ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Endothelial Function ◽  
Spontaneously Hypertensive Rats ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Terminalia Superba

scholarly journals Argan (Argania spinosa) oil lowers blood pressure and improves endothelial dysfunction in spontaneously hypertensive rats

2004 ◽  
Vol 92 (6) ◽  
pp. 921-929 ◽  
Author(s):  
Hicham Berrougui ◽  
Maria Alvarez de Sotomayor ◽  
Concepción Pérez-Guerrero ◽  
Abdelkader Ettaib ◽  
Mohamed Hmamouchi ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Endothelial Function ◽  
Spontaneously Hypertensive Rats ◽  
Mesenteric Arteries ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Argania Spinosa ◽  
Beneficial Effects ◽  
Argan Oil ◽  
Wistar Kyoto

Traditionally hand-pressed argan oil, obtained from Argania spinosa seeds, is eaten raw in south-west Morocco; its rich composition of tocopherols, MUFA and PUFA make a study of its actions on risk factors for CVD, such as hypertension, interesting. The effects of 7 weeks of treatment with argan oil (10ml/kg) on the blood pressure and endothelial function of spontaneously hypertensive rats (SHR) and normotensive Wistar–Kyoto rats were investigated. Systolic blood pressure and heart rate were measured every week by the tail-cuff method and endothelial function was assessed by carbachol (10−8 to 10−4m)-induced relaxations of aortic rings and small mesenteric arteries pre-contracted with phenylephrine. Argan-oil administration reduced the mean blood pressure of SHR after the fifth week of treatment (P<0·05) and increased (P<0·01) the endothelial responses of arteries from SHR. The NO synthase inhibitor, l-N-ω-nitroarginine (3×10−5m) revealed a greater participation of NO in the relaxant effect after the treatment. When cyclooxygenase (COX) was blocked with indomethacin (10−5m), an involvement of COX products in the endothelium-dependent response was characterized. Enzyme immunoassay of thromboxane B2 showed a significant decrease (P<0·05) in the release of thromboxane A2 in both aorta and small mesenteric artery after argan-oil treatment of SHR. Experiments in the presence of the thromboxane A2–prostaglandin H2 receptor antagonist ICI 192, 605 (10−5m) confirmed this result. Results after incubation with the antioxidants superoxide dismutase and catalase suggested that a decreased oxidative stress might contribute to explain the beneficial effects of argan-oil treatment.

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