Expression, purification and characterization of factor IX derivatives using a novel vector system

2006 ◽  
Vol 50 (2) ◽  
pp. 196-202 ◽  
Author(s):  
Likui Yang ◽  
Kota Gopalakrishna ◽  
Chandrashekhara Manithody ◽  
Alireza R. Rezaie
Keyword(s):  
Factor Ix ◽  
Vector System ◽  
Purification And Characterization

scholarly journals Purification and characterization of an abnormal factor IX (Christmas factor) molecule. Factor IX Chapel Hill.

1978 ◽  
Vol 62 (5) ◽  
pp. 1078-1085 ◽  
Author(s):  
K S Chung ◽  
D A Madar ◽  
J C Goldsmith ◽  
H S Kingdon ◽  
H R Roberts
Keyword(s):  
Factor Ix ◽  
Purification And Characterization ◽  
Chapel Hill

scholarly journals Construction, Rescue, and Characterization of Vectors Derived from Ovine Atadenovirus

2003 ◽  
Vol 77 (22) ◽  
pp. 11941-11951 ◽  
Author(s):  
Peter Löser ◽  
Christian Hofmann ◽  
Gerald W. Both ◽  
Wolfgang Uckert ◽  
Moritz Hillgenberg
Keyword(s):  
Gene Expression ◽  
Mammalian Cells ◽  
Human Factor ◽  
Insertion Site ◽  
Factor Ix ◽  
Vector System ◽  
Virus Rescue ◽  
Human Factor Ix

ABSTRACT Gene transfer vectors derived from ovine atadenovirus type 7 (OAdV) can efficiently infect a variety of mammalian cells in vitro and in vivo to deliver and express transgenes. However, early OAdV vectors were designed on human mastadenovirus principles prior to the complete characterization of OAdV genes and transcripts. The distinctive arrangement of the OAdV genome has suggested ways to improve OAdV vector design and utility. We therefore developed a cosmid-based approach that allows efficient construction of recombinant ovine atadenovirus genomes in which the transgene is inserted at one of three sites. Viruses were rescued by transfection of viral DNA into a new ovine fetal skin fibroblast producer cell line, HVO156. The suitability of the three insertion sites was compared with respect to virus rescue efficiency, gene expression levels, and genetic stability of the vectors. We found that one vector with a transgene inserted at site 1, between the pVIII and fiber genes, was unstable. Only one vector that carried a transgene at site 2, near the right end of the genome, together with a nearby deletion was rescued. In contrast, several vectors with different transgenes inserted in site 3, between the E4 and RH transcription units, were repeatedly rescued, and these vectors were stable over at least four passages. Transgene orientation in site 3 had only little effect on expression. Finally, a vector carrying a human factor IX cDNA at site 3, when administered intravenously, produced nearly physiological levels of human factor IX in mice. The availability of an efficient method for vector construction and the identification of a new insertion site for virus rescue and gene expression substantially enhance the utility of the OAdV vector system.

Purification and characterization of human factor IX

1975 ◽  
Vol 7 (3) ◽  
pp. 451-459 ◽  
Author(s):  
L-O. Andersson ◽  
H. Borg ◽  
M. Miller-Andersson
Keyword(s):  
Human Factor ◽  
Factor Ix ◽  
Purification And Characterization ◽  
Human Factor Ix

Purification and characterization of Epstein-Barr virus gp340/220 produced by a bovine papillomavirus virus expression vector system

Vaccine ◽  
1992 ◽  
Vol 10 (11) ◽  
pp. 777-782 ◽  
Author(s):  
Monika Madej ◽  
Margaret J. Conway ◽  
Andrew J. Morgan ◽  
Jill Sweet ◽  
Lesley Wallace ◽  
...  
Keyword(s):  
Epstein Barr Virus ◽  
Expression Vector ◽  
Vector System ◽  
Bovine Papillomavirus ◽  
Purification And Characterization ◽  
Barr Virus ◽  
Epstein Barr ◽  
Virus Expression

Purification and characterization of phosphoglucomutase from peas

1994 ◽  
Vol 92 (3) ◽  
pp. 479-486 ◽  
Author(s):  
Cynthia M. Galloway ◽  
W. Mack Dugger
Keyword(s):  
Purification And Characterization
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