High-level expression and purification of a nonmitogenic form of human acidic fibroblast growth factor in Escherichia coli

2005 ◽  
Vol 42 (1) ◽  
pp. 7-11 ◽  
Author(s):  
Xiaoping Wu ◽  
Zhijian Su ◽  
Xiaokun Li ◽  
Qing Zheng ◽  
Yadong Huang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Fibroblast Growth ◽  
Expression And Purification ◽  
Acidic Fibroblast Growth Factor ◽  
High Level Expression ◽  
High Level

High-level expression of human fibroblast growth factor-9 N33 in Escherichia coli

1995 ◽  
Vol 80 (4) ◽  
pp. 327-333 ◽  
Author(s):  
Masato Kuriyama ◽  
Jyun-Ichi Sakamoto ◽  
Masanori Nakatu ◽  
Tsutomu Kurokawa ◽  
Hidekazu Sawada
Keyword(s):  
Escherichia Coli ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Human Fibroblast ◽  
Fibroblast Growth ◽  
High Level Expression ◽  
High Level

scholarly journals Detection of high levels of heparin binding growth factor-1 (acidic fibroblast growth factor) in inflammatory arthritic joints.

1990 ◽  
Vol 110 (4) ◽  
pp. 1417-1426 ◽  
Author(s):  
H Sano ◽  
R Forough ◽  
J A Maier ◽  
J P Case ◽  
A Jackson ◽  
...  
Keyword(s):  
T Cell ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Disease Process ◽  
Fibroblast Growth ◽  
Heparin Binding ◽  
Acidic Fibroblast Growth Factor ◽  
Streptococcal Cell Wall ◽  
Fos Protein ◽  
High Level

The synovium from patients with rheumatoid arthritis (RA) and LEW/N rats with streptococcal cell wall (SCW) arthritis, an experimental model resembling RA, is characterized by massive proliferation of synovial connective tissues and invasive destruction of periarticular bone and cartilage. Since heparin binding growth factor (HBGF)-1, the precursor of acidic fibroblast growth factor (FGF), is a potent angiogenic polypeptide and mitogen for mesenchymal cells, we sought evidence that it was involved in the synovial pathology of RA and SCW arthritis. HBGF-1 mRNA was detected in RA synovium using the polymerase chain reaction technique, and its product was immunolocalized intracellularly in both RA and osteoarthritis (OA) synovium. HBGF-1 staining was more extensive and intense in synovium of RA patients than OA and correlated with the extent and intensity of synovial mononuclear cell infiltration. HBGF-1 staining also correlated with c-Fos protein staining. In SCW arthritis, HBGF-1 immunostaining was noted in bone marrow, bone, cartilage, synovium, ligamentous and tendinous structures, as well as various dermal structures and developed early in both T-cell competent and incompetent rats. Persistent high level immunostaining of HBGF-1 was only noted in T-cell competent rats like the disease process in general. These observations implicate HBGF-1 in a multitude of biological functions in inflammatory joint diseases.

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