scholarly journals Assessing the oxidative modification of proteins in inflamed placenta combined with iron deficiency anemia in the pregnant through histochemical method with bromophenol blue based on Mikel Calvo

2017 ◽  
Vol 2 (5) ◽  
pp. 215
Author(s):  
Y. Karliichuk ◽  
V. Ilika
Keyword(s):  
Iron Deficiency ◽  
Iron Deficiency Anemia ◽  
Oxidative Modification ◽  
Histochemical Method ◽  
Deficiency Anemia ◽  
Bromophenol Blue ◽  
Oxidative Modification Of Proteins

scholarly journals Histochemical Study of the Processes of Protein Oxidative Modification and Limited Proteolysis in the Endothelium of Myometrial Vessels in the Projection of the Utero-Placental Bed during Iron-Deficiency Anemia in Pregnancy

2021 ◽  
Vol 6 (4) ◽  
pp. 58-63
Author(s):  
O. A. Tiulienieva ◽  
Keyword(s):  
Endothelial Dysfunction ◽  
Iron Deficiency ◽  
Pregnant Women ◽  
Iron Deficiency Anemia ◽  
Limited Proteolysis ◽  
Oxidative Modification ◽  
Deficiency Anemia ◽  
Bromophenol Blue ◽  
Oxidative Modification Of Proteins ◽  
Protein Oxidative Modification

Utero-placental bed is the cumulation of gestationally altered endometrium at the place of ovum attachment to the uterine wall. As far as the protein oxidative modification and limited proteolysis in iron deficiency anemia are due to the fact that in conditions of hypoxia, free radical processes in the blood and tissues are enhanced, and iron deficiency is additionally able to cause hemodynamic disorders because of endothelial dysfunction in the vessels of the utero-placental area. The purpose of the study was to establish histochemical features of protein oxidative modification and limited proteolysis in the endotheliocytes of myometrial vessels in the projection of the utero-placental area depending on the degree of iron deficiency anemia in pregnant women. Materials and methods. By histochemical methods of Mikel Calvo, using reactions with bromophenol blue on "acidic" and "basic" proteins, and the method of A. Yasumа and T. Ichikawa, ninhydrin-Schiff reaction to free amino groups of proteins to assess the degree of limited proteolysis, in combination with computer microspectrophotometry and microdensitometry, quantitative characteristics of oxidative modification of proteins and limited proteolysis in endotheliocytes of myometrial segments of the utero-placental vessels in iron deficiency anemia of pregnant women were established. 74 biopsies of the observed uterine-placental area in physiological pregnancy and gestation based on iron deficiency anemia of I, II and III degrees of severity were investigated. Results and discussion. In physiological pregnancy, the intensity of oxidative modification of proteins and limited proteolysis is the lowest in endotheliocytes of myometrial segments of the spiral arteries and the highest one is in the endothelium of the vessels of the microcirculatory tract of the utero-placental area. In gestations based on iron deficiency anemia, intensification of proteins oxidative modification and limited proteolysis in the endothelium of all types of myometrial vessels of the projection of the utero-placental bed correlates with the severity of anemia. Iron deficiency anemia greatly affects the modification of proteins in the endothelial cells mostly of myometrial segments of spiral arteries of the placental bed. Conclusion. Intensification of protein oxidative modification and limited proteolysis in endotheliocytes of all type vessels of myometrium of the uterine-placental area formed by iron deficiency can be considered as a significant factor of endothelial dysfunction and a predictor of hemodynamic disorders of the placental bed

scholarly journals HISTOCHEMICAL EVALUATION OF THE PROCESSES OF PROTEIN OXIDATIVE MODIFICATION IN THE FIBRINOID OF THE PLACENTAL BASAL LAMINA COMBINED WITH PLACENTAL INFLAMMATION AND IRONDEFICIENCY ANEMIA OF GRAVIDAS

2016 ◽  
Vol 15 (4) ◽  
pp. 48-51
Author(s):  
V. V. Ilika ◽  
I. S. Davydenko ◽  
O. M. Davydenko
Keyword(s):  
Iron Deficiency ◽  
Chronic Inflammation ◽  
Iron Deficiency Anemia ◽  
Basal Lamina ◽  
Oxidative Modification ◽  
Deficiency Anemia ◽  
Bromophenol Blue ◽  
Protein Oxidative Modification ◽  
Placental Inflammation ◽  
Acute Form

The results of the research based on histochemical reactions with Bromophenol Blue on “acidic” and “basic” proteins according to Mikel Calvo method have found the following. A moderate intensification of the processes of protein oxidative modification is observed in iron-deficiency anemia in the fibrinoid of basal lamina in comparison with physiological pregnancy. There is a significant increase in the intensity of protein oxidative modification in the fibrinoid of basal lamina except acute form of chorioamnionitis. Underlying iron-deficiency anemia in various forms of inflammation in the placenta significantly affects the processes of protein oxidative modification in the fibrinoid of basal lamina in case of chronic inflammation of the placenta only.

scholarly journals Histochemical Evaluation of the Processes of Protein Oxidative Modification in the Extravillous Cytotrophoblast of the Utero-Placental Bed during Iron-Deficiency Anemia in Pregnancy

2021 ◽  
Vol 6 (1) ◽  
pp. 46-51
Author(s):  
O. A. Tiulienieva ◽  
◽  
I. S. Davydenko ◽  
A. V. Hoian ◽  
V. O. Tiulienieva
Keyword(s):  
Iron Deficiency ◽  
Pregnant Women ◽  
Iron Deficiency Anemia ◽  
Oxidative Modification ◽  
Extravillous Trophoblast ◽  
Deficiency Anemia ◽  
Basic Proteins ◽  
Protein Oxidative Modification ◽  
Acidic Proteins ◽  
Extravillous Cytotrophoblast

Utero-placental bed is the cumulation of gestationally altered endometrium at the place of ovum attachment to the uterine wall. The key mechanism of this process is the cytotrophoblastic invasion. During iron deficiency anemia, an increase in the specific volume of the extravascular invasive trophoblast is taking place. Concern for the protein oxidative modification in iron deficiency anemia is due to the fact that in conditions of hypoxia, free radical processes in the blood and tissues are enhanced, and iron deficiency is additionally able to modify this problem. The purpose of the study was to establish the histochemical features of the processes of protein oxidative modification in the fractions of extravillous cytotrophoblast of the utero-placental bed depending on the degree of iron deficiency anemia in pregnant women. Material and methods. Quantitative characteristics of protein oxidative modification in the extravillous trophoblast of the utero-placental bed of pregnant women with iron deficiency anemia by means of the histochemical method using reactions with Bromophenol Blue on “acidic” and “basic” proteins according to Mikel Calvo method and computer microdensitometry. We studied 74 biopsies of the utero-placental bed of pregnant women with iron deficiency anemia of I, II and III degrees. The term of gestation was 37-40 weeks. Results and discussion. During physiological pregnancy, the ratio between "acidic" and "basic" proteins in trophoblast cells, even normally, is characterized by a predominance of "acidic" proteins, and evenly in both intravascular and extravascular fractions of cytotrophoblast. Intensification of processes of protein oxidative modification in the cytotrophoblast of the utero-placental bed during iron deficiency anemia of I-II degrees can be assessed as moderate, with an increase in the cells of the endothelium-replacing fraction of cytotrophoblast compared with the interstitial. In conditions of anemia of III degree, a significant predominance of "acidic" proteins in the intravascular cytotrophoblast was noted. Conclusion. During the physiological pregnancy, the intensity of protein oxidative modification was equal in all fractions of the extravillous cytotrophoblast in the utero-placental bed. In the case of gestation with iron deficiency anemia, significant intensification of the protein oxidative modification in the extravillous cytotrophoblast correlated with the severity of anemia. Background iron deficiency anemia significantly affected the processes of protein oxidative modification in the endothelium-replacing cytotrophoblast

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