scholarly journals Tamoxifen effects on respiratory chain complexes and creatine kinase activities in an animal model of mania

2011 ◽  
Vol 98 (2) ◽  
pp. 304-310 ◽  
Author(s):  
Morgana Moretti ◽  
Samira S. Valvassori ◽  
Amanda V. Steckert ◽  
Natalia Rochi ◽  
Joana Benedet ◽  
...  
Keyword(s):  
Animal Model ◽  
Creatine Kinase ◽  
Respiratory Chain ◽  
Respiratory Chain Complexes ◽  
Chain Complexes

Corrigendum to “Tamoxifen effects on respiratory chain complexes and creatine kinase activities in an animal model of mania”

2011 ◽  
Vol 99 (1) ◽  
pp. 112
Author(s):  
Morgana Moretti ◽  
Samira S. Valvassori ◽  
Amanda V. Steckert ◽  
Natalia Rochi ◽  
Joana Benedet ◽  
...  
Keyword(s):  
Animal Model ◽  
Creatine Kinase ◽  
Respiratory Chain ◽  
Respiratory Chain Complexes ◽  
Chain Complexes

Effects of maintenance electroshock on mitochondrial respiratory chain and creatine kinase activities in the rat brain

2012 ◽  
Vol 24 (5) ◽  
pp. 275-285
Author(s):  
Gislaine Z. Réus ◽  
Roberto B. Stringari ◽  
Gislaine T. Rezin ◽  
Daiana P. Pezente ◽  
Giselli Scaini ◽  
...  
Keyword(s):  
Creatine Kinase ◽  
Energy Metabolism ◽  
Rat Brain ◽  
Respiratory Chain ◽  
Brain Area ◽  
Mitochondrial Respiratory Chain ◽  
Respiratory Chain Complexes ◽  
Chain Complexes ◽  
Male Wistar Rats ◽  
Mitochondrial Respiratory

Réus GZ, Stringari RB, Rezin GT, Pezente DP, Scaini G, Maggi DD, De-Nês BT, Streck EL, Quevedo J, Feier G. Effects of maintenance electroshock on mitochondrial respiratory chain and creatine kinase activities in the rat brain.Objective: Electroconvulsive therapy is used efficacious treatment for a variety of complicated psychiatric disorders and evidences have indicated that energy metabolism impairment may be involved in pathophysiology and treatment of mood disorders. This work was performed to determine creatine kinase and mitochondrial respiratory chain activities at different times after the maintenance electroconvulsive shock (ECS).Methods: Male Wistar rats received a protocol mimicking therapeutic of maintenance or simulated ECS (sham) and were subsequently sacrificed immediately after, 48 h and 7 days after the last maintenance ECS. We measured creatine kinase and mitochondrial respiratory chain activities in the prefrontal cortex, hippocampus, cortex, cerebellum and striatum.Results: Our results showed that maintenance ECS alter respiratory chain complexes and creatine kinase activities in the rat brain, but these effects were related to brain area and time after the ECS, in which the animal were killed.Conclusion: Finally, these findings further support the hypothesis that alteration on the energy metabolism could be involved in the therapeutic or adverse effects of ECS.

Effects of N-Acetylcysteine/Deferoxamine, Taurine and RC-3095 on Respiratory Chain Complexes and Creatine Kinase Activities in Rat Brain After Sepsis

2009 ◽  
Vol 35 (4) ◽  
pp. 515-521 ◽  
Author(s):  
Omar J. Cassol ◽  
Gislaine T. Rezin ◽  
Fabrícia C. Petronilho ◽  
Giselli Scaini ◽  
Cinara L. Gonçalves ◽  
...  
Keyword(s):  
Creatine Kinase ◽  
Rat Brain ◽  
Respiratory Chain ◽  
Respiratory Chain Complexes ◽  
Chain Complexes

scholarly journals Impairment of Electron Transfer Chain Induced by Acute Carnosine Administration in Skeletal Muscle of Young Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
José Roberto Macarini ◽  
Soliany Grassi Maravai ◽  
José Henrique Cararo ◽  
Nádia Webber Dimer ◽  
Cinara Ludvig Gonçalves ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Electron Transfer ◽  
Creatine Kinase ◽  
Respiratory Chain ◽  
Atp Depletion ◽  
Neurological Dysfunction ◽  
Control Group ◽  
Mrna Levels ◽  
Respiratory Chain Complexes ◽  
Chain Complexes

Serum carnosinase deficiency is an inherited disorder that leads to an accumulation of carnosine in the brain tissue, cerebrospinal fluid, skeletal muscle, and other tissues of affected patients. Considering that high levels of carnosine are associated with neurological dysfunction and that the pathophysiological mechanisms involved in serum carnosinase deficiency remain poorly understood, we investigated thein vivoeffects of carnosine on bioenergetics parameters, namely, respiratory chain complexes (I–III, II, and II-III), malate dehydrogenase, succinate dehydrogenase, and creatine kinase activities and the expression of mitochondrial-specific transcription factors (NRF-1, PGC-1α, andTFAM) in skeletal muscle of young Wistar rats. We observed a significant decrease of complexes I–III and II activities in animals receiving carnosine acutely, as compared to control group. However, no significant alterations in respiratory chain complexes, citric acid cycle enzymes, and creatine kinase activities were found between rats receiving carnosine chronically and control group animals. As compared to control group, mRNA levels ofNRF-1, PGC-1α, andTFAMwere unchanged. The present findings indicate that electron transfer through the respiratory chain is impaired in skeletal muscle of rats receiving carnosine acutely. In case these findings are confirmed by further studies and ATP depletion is also observed, impairment of bioenergetics could be considered a putative mechanism responsible for the muscle damage observed in serum carnosinase-deficient patients.

Relationships between the activity of respiratory-chain complexes in pre- (biopsy) or post-slaughter muscle samples and feed efficiency in random-bred Ghezel lambs

2017 ◽  
Vol 57 (8) ◽  
pp. 1674
Author(s):  
M. J. Zamiri ◽  
R. Mehrabi ◽  
G. R. Kavoosi ◽  
H. Rajaei Sharifabadi
Keyword(s):  
Respiratory Chain ◽  
Feed Intake ◽  
Enzyme Activities ◽  
Feed Efficiency ◽  
Average Daily Gain ◽  
Chain Complex ◽  
Respiratory Chain Complex ◽  
Respiratory Chain Complexes ◽  
Chain Complexes ◽  
Daily Gain

The present study was conducted to determine the relationship between the activity of mitochondrial respiratory chain complexes in pre- and post-slaughter muscle samples and residual feed intake (RFI) in Ghezel male lambs born as a result of random mating. The study was based on the hypothesis that random-bred lambs with lower feed (or higher) RFI have lower (or higher) respiratory chain-complex activity in muscle samples. Lambs (n = 30) were fed a diet consisting of 70% concentrate and 30% alfalfa hay during a 70-day period. Individual feed intake and average daily gain were recorded to calculate the RFI, feed-conversion ratio (FCR) and adjusted FCR (aFCR). On the basis of these calculations, the lambs were classified into low and high groups for RFI, with FCR and aFCR (n = 22) being one standard deviation above or below the means; this was corroborated by Student’s t-test (P < 0.01). At the end of the experiment, a 10-g biopsy sample was taken from the posterior side of the left femoral biceps. After 24 h, the lambs were slaughtered, and a sample from the posterior side of the right femoral biceps was dissected for determination of mitochondrial protein and respiratory chain-complex activities (Complexes I–V). The RFI was not correlated with the metabolic bodyweight and average daily gain, but was positively correlated (r = 0.56) with the average daily feed intake (P < 0.01); mean daily feed intake in the low-RFI group was 200 g less than that in the high-RFI group. The FCR and aFCR were not significantly (P > 0.05) correlated with average daily feed intake (r = 0.39 and r = 0.36 respectively), but showed a negative correlation (P < 0.01) with average daily gain (r = –0.73 and r = –0.76 respectively). Although very high negative correlations were recorded between the activities of all five respiratory-chain complexes and RFI in muscle samples obtained before (–0.91 to –0.97) and after (–0.92 to –0.97) slaughter, Complexes I and V showed small negative correlations (–0.40) with FCR or aFCR (P < 0.05). Enzyme activities of the respiratory-chain Complexes I, III and V were not significantly different between the pre- and post-slaughter biopsy samples; however, the enzyme activities of respiratory-chain Complexes II and IV were slightly higher in post-slaughter samples (P < 0.01). These results suggested that it may be possible to use the enzymatic activity of respiratory-chain complexes in muscle biopsy samples for screening of lambs for RFI, providing a useful procedure for genetic selection of lambs for this component of feed efficiency. These encouraging results need to be verified in further experiments using other sheep breeds and a larger number of lambs.

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