THE IMMUNOLOGICAL PROFILE OF AgHBs IN SCREENING TESTS OF BLOOD DONORS, IN BIHOR COUNTY ROMANIA

2018 ◽  
Vol 25 (3) ◽  
pp. 238
Author(s):  
Olivia Burta ◽  
Catalin Nicolae ◽  
Sebastian Stanciu
Keyword(s):  
Blood Donors ◽  
Screening Tests ◽  
Immunological Profile

Predictive value of past and current screening tests for syphilis in blood donors: changing from a rapid plasma reagin test to an automated specific treponemal test for screening

Transfusion ◽  
1999 ◽  
Vol 39 (2) ◽  
pp. 206-211 ◽  
Author(s):  
John Aberle-Grasse ◽  
Sharyn L. Orton ◽  
Edward Notari ◽  
Lynne P. Layug ◽  
Richard G. Cable ◽  
...  
Keyword(s):  
Predictive Value ◽  
Blood Donors ◽  
Screening Tests ◽  
Rapid Plasma Reagin ◽  
Current Screening

How Many Bloods will a ‘HIVCHEK’ Check? Multiple Tests for HIV Antibody for a Single Screening Kit

1992 ◽  
Vol 22 (4) ◽  
pp. 151-154 ◽  
Author(s):  
G J Wannan ◽  
M J Cranefield ◽  
W A M Cutting ◽  
P R Fischer ◽  
F D Hargreaves ◽  
...  
Keyword(s):  
Blood Donors ◽  
Screening Tests ◽  
Serum Samples ◽  
Hiv Seroprevalence ◽  
Multiple Tests ◽  
Test Kit ◽  
Highly Sensitive ◽  
Multiple Sample ◽  
Sample Method ◽  
Hiv 1

Detection of HIV infection in blood donors or populations is usually by testing sera for antibodies to HIV-1 and HIV-2. Screening tests are now highly sensitive and specific, but still expensive and scarce in Africa. We tested the commercially available kits ‘HIVCHEK 1 + 2′ in two field laboratories, on specimens from blood donors and antenatal women in rural Zaire. We describe a method of using one test kit for up to five serum samples, saving money and time. In 491 antenatal mothers in Eastern Zaire, among whom the HIV seroprevalence was 3.3%, we compared ‘HIVCHEK’ results with results obtained by ELISA and Western blot. The ‘HIVCHEK’ multiple-sample method had a sensitivity of 82% and a specificity of 99.6%. In an area with an HIV seroprevalence of <4%, using ‘HIVCHEK’ by the multiple sample method would lead to a saving of about £2400 for every 1000 individuals tested.

scholarly journals Evaluating laboratory screening tests for malaria on blood donors candidates to reduce the risk of transfusion-transmitted malaria in an endemic area of Indonesia

2021 ◽  
Vol 30 (3) ◽  
pp. 191-7
Author(s):  
Nethasia Louhenapessy ◽  
Ria Syafitri Evi Gantini ◽  
Susan Rahayu ◽  
Elisabeth Lilipory ◽  
Heri Wibowo ◽  
...  
Keyword(s):  
Endemic Area ◽  
Blood Donors ◽  
Rapid Test ◽  
Clinical Malaria ◽  
Screening Tests ◽  
Red Cross ◽  
Policy And Practice ◽  
Laboratory Screening ◽  
Malaria Antigen ◽  
History Of

BACKGROUND Laboratory screening of blood donors for malaria has not been routinely performed in Indonesia. Current policy and practice simply exclude donors based on a history of active clinical malaria. This study was aimed to evaluate laboratory screening tests for malaria among blood donors in an endemic area of Indonesia. METHODS The study was conducted on 550 consecutive blood samples withdrawn from volunteer donors at the Red Cross Blood Transfusion Unit in Ambon city using microscopic and rapid diagnostic tests for antigen as well as for antibody. Furthermore, 248 of those 550 samples were also tested for the presence of malaria DNA using 18S rRNA marker. Statistical analysis was done descriptively using SPSS software version 15 (SPSS Inc., USA). RESULTS The overall malaria positivity rate among the donors was 4.5% (25/550). None of the specimens tested using microscopy or rapid test for malaria antigen assay were positive. However 22 (4.0%) samples were positive for malaria antibody against Plasmodium falciparum; while 3 (1.2%) were positive by PCR. CONCLUSIONS Laboratory testing for blood donors may be used to prevent transfusiontransmitted malaria in an endemic area of Indonesia.

scholarly journals Evaluation of positive serological screening test rates in blood donors with ABO and Rh type blood groups

2019 ◽  
Vol 6 (09) ◽  
pp. 4592-4595
Author(s):  
Canan Eren
Keyword(s):  
Blood Transfusion ◽  
Blood Donors ◽  
Hepatitis B Surface Antigen ◽  
Blood Groups ◽  
Pathogen Transmission ◽  
Screening Tests ◽  
Serum Samples ◽  
Serological Screening ◽  
Significant Difference ◽  
Anti Hiv

Background: Blood transfusion is a life-saving method in emergencies. However, it also carries the risk of pathogen transmission, which is called transfusion transmitted infections (TTIs). All donated blood samples were screened for hepatitis B surface antigen (HBsAg), anti-hepatitis C virus antibody (anti-HCV), anti-human immunodeficiency virus antigens (anti-HIV½) and syphilis using Venereal Disease Research Laboratory (VDRL) test. This study was designed and carried out to determine the seroprevalences of TTIs among blood donors with different ABO and Rh blood groups. Identification of a relation between different blood groups and TTIs was anticipated. Methods: All blood donors data obtained from the Blood Center of Marmara University Pendik Training and Research Hospital between January 2013 and October 2018 are analyzed retrospectively. Serum samples were examined for HBsAg, anti-HCV, anti-HIV½, and VDRL. Results: HBsAg and anti-HIV½ positivity positivity rates were not statistically different according to ABO blood groups (p>0.05). Anti-HCV positivity rates are partially statistically significant (p<0.05). While the incidence of anti-HCV in patients with AB Rh (+) cases was significantly higher than that of 0 Rh (+), A Rh (+) and AB Rh (+) groups, no significant difference was found for the other analyzed blood groups (p>0.05). VDRL positivity rates did not differ significantly (p>0.05). Seropositive cases, as reported by Rh type blood group; HBsAg, anti-HCV, anti-HIV½, and VDRL positivity rates were not statistically significant (p>0.05). Conclusions: No significant relationship was found among TTIs and ABO and Rh type blood groups. For blood transfusion safety, screening tests should always and carefully be performed.

scholarly journals A Comparative Study of Solubility Based Sickle Cell Screening Versus Genomic Method in Blood Donors

Blood ◽  
2015 ◽  
Vol 126 (23) ◽  
pp. 1148-1148
Author(s):  
Lohith shakaladevanapura Bachegowda ◽  
Yelena Ginzburg ◽  
Sunitha Vege ◽  
Connie Westhoff
Keyword(s):  
Sickle Cell ◽  
False Positive ◽  
Blood Donors ◽  
Gene Sequencing ◽  
Molecular Methods ◽  
Alternative Methods ◽  
Screening Tests ◽  
Carrier Status ◽  
Common Mutation ◽  
Pcr Multiplex

Abstract Background: Sickle cell trait (SCT) is more common in minority African-Americans (AA) and Hispanic blood donors. Screening donors for Sickle Hemoglobin (HbS) carrier status (SCT) can be useful, especially in managing the transfusion needs of neonates and patients with sickle cell disease. Currently, the Sickledex test, based on the principle of relative insolubility of HbS in phosphate buffers, is routinely used for HbS donor screening. However, there are several limitations including the inability to perform high-throughput testing by automation as donor centers target larger numbers of minority blood donors. False positives results due to hyperlipidemia, dysglobulinemia and higher peripheral blood hematologic counts have also been reported. Hence alternative methods to identify SCT donors are highly desirable in blood centers. The purpose of this study was to validate testing, and to identify rates of False Positive (FP) and False Negative (FN) sickledex screening in blood donors by verifying the presence or absence of the most common mutation seen with HbS production using molecular methods. The comparison could offer evidence to use molecular methods as a test of record to manage HbS carriers at blood centers. Methods and Results Between Jan 2012 till Dec 2014, 200 sickledex screening tests were done daily, totaling approximately 90,000 donors self-identifying as Caucasians and 60,000 donors identifying as African American or Hispanic tested during the study period. Amongst Caucasians tested, 154 had a positive sickledex screen result. Since the prevalence of SCT is less common in the Caucasian population, we hypothesized several of these Caucasian donors could have a false positive screening test. All 154 sickledex positive Caucasian donor samples were prospectively tested for the HbS mutation detection with molecular methods. BioArray Human Erythrocyte Antigen (HEA) Bead Chip DNA assay with PCR multiplex based technology was used to detect the HbS mutation (β-globin change -173 A>T, more commonly designated 20A>T). Of the 154 sickledex + Caucasians, 108 (70%) donors tested negative (FP Screen) and 46 (30%) tested positive for the HbS mutation. A retrospective review of sickledex results in our electronic database for minority donors (n-3431) who also had HEA BeadChip assay was performed to determine concordance. Of 3431 minority donors tested, only 2 (0.0006%) had a positive sickledex screening and were negative by HEA, with the remaining negative by both the sickledex screen and HEA tests. Among minority donors (n= 254) testing + for HbS by HEA, 3% (8/254) were negative by sickledex screening. To establish concordance of HEA assay with high performance liquid chromatographic (HPLC) assay, two separate cohorts were evaluated. Initially 12 sickle screen and HEA positive donors was subject to HPLC analysis, which confirmed SCT status in all 12 samples. Subsequently sickledex screen positive but HEA negative samples (n= 40), were subject to HPLC. All 40 showed absence of SCT by HPLC. Finally, 58 random sickledex + samples had gene sequencing and HEA performed in parallel. Out of 58 samples, 54 showed a positive HbS mutation by HEA with A>T (SCT) nucleotide change noted with gene sequencing. In the remaining 4 samples, HEA was negative with gene sequencing showing (3 T>T and in 1C>T) change at nucleotide 163 associated with Hemoglobin Okayama. Conclusion: The HEA bead chip assay testing for the HbS mutation showed 100% concordance with HPLC and gene sequencing in samples studied. Molecular methods can process multiple samples in a single setting to detect SCT. FP sickledex screening results in Caucasians needs confirmatory testing for the HbS mutation to avoid unnecessary donor notification and deferral. The association, if any, of false positive sickledex screening in Caucasian donors with the Hgb Okayama mutation is under further investigation. Disclosures No relevant conflicts of interest to declare.

scholarly journals The Statistical Analysis of Factors Explaining the Intention of Public for Blood Donation in Jamshoro, Sindh

2021 ◽  
Vol 7 (2) ◽  
pp. 13-20
Author(s):  
Nazia Parveen Gill ◽  
Fozia Parveen Panhwar ◽  
Sunbul Naeem Cheema ◽  
Raja Muhammad Ilyas
Keyword(s):  
Blood Donors ◽  
Blood Donation ◽  
Sampling Technique ◽  
Final Analysis ◽  
Screening Tests ◽  
Cluster Sampling ◽  
Chi Square ◽  
Willingness To Donate ◽  
Chi Square Test ◽  
The Media

Purpose: The purpose of this research is to determine different factors explaining the intention of public in context of blood donation in the district of Jamshoro, Sindh. Methodology: The data were collected of 400 samples from four different tehsils of Jamshoro. The data was collected through well-structured questionnaire. The survey was conducted in 2019 and cluster sampling technique was used. The internal consistency of the questionnaire was examined, and Chi-Square test was applied for final analysis. Findings: The public's willingness to donate blood is limited, according to this study (χ2 (1) = 0.88, p=0.39). The media does not perform any significant role in awareness generating and educating the general public about the importance of donating blood (χ2 (1) = 24.35, p=0.001). Women make up a small percentage of blood donors in society (χ2 (1) = 0.05, p=0.82). This research also compares blood donors and non-donors based on gender and age. The contribution of blood donors in younger age was higher (χ2 (3) = 19.31, p=0.01) in males (χ2 (1) = 27.98, p=0.001). Conclusion: The awareness of blood donation was higher in males, higher education, and young age peoples (18-28) years. Furthermore, the awareness about blood donation should be made known through electronic and print media along with the educational programs at educational institutions. Blood bank lab assistants should be given training to keep up to date with the latest information on blood donation, samples, and screening tests.

Transfusion-transmissible dengue infections

2020 ◽  
Vol 114 (11) ◽  
pp. 866-882 ◽  
Author(s):  
Lakshika Perera ◽  
Nandrani De Zoysa ◽  
Umesh Jayarajah ◽  
Nilanthi Senanayake ◽  
Ishan De Zoysa ◽  
...  
Keyword(s):  
Global Health ◽  
Literature Search ◽  
Blood Donors ◽  
Developed Countries ◽  
Screening Tests ◽  
Safe Blood ◽  
Selection Stage ◽  
Global Health Problem ◽  
Developing And Developed Countries ◽  
Apparently Healthy

Abstract Dengue is an important global health problem and is endemic in many developing and developed countries. Transmission of dengue may occur in several ways and information on transfusion-transmitted dengue is limited. We conducted a literature search on transfusion-related dengue using the PubMed, Scopus, Embase and Google Scholar databases and have summarized the findings. A number of apparently healthy blood donors have been found to be infected with the dengue virus (DENV) and thus may transmit the virus onto recipients of this blood. It is not possible to identify and exclude such donors at the donor selection stage and thus reliable screening tests should be available in highly endemic areas to ensure a safe blood supply.

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