THE EFFECTS OF POTASSIUM-CYANIDE ON FUNCTIONAL RECOVERY OF ISOLATED RAT HEART AFTER ISCHEMIA AND REPERFUSION: ROLE OF OXIDATIVE STRESS

2018 ◽  
Vol 25 (3) ◽  
pp. 177 ◽  
Author(s):  
Vladimir Lj Jakovljevic ◽  
Anica Petkovic ◽  
Jovana Bradic ◽  
Jovana Jeremic ◽  
Tamara Nikolic Turnic ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Functional Recovery ◽  
Rat Heart ◽  
Potassium Cyanide ◽  
Isolated Rat Heart ◽  
Ischemia And Reperfusion ◽  
Isolated Rat

scholarly journals Modulation ofN-methyl-d-aspartate receptors in isolated rat heart

2017 ◽  
Vol 95 (11) ◽  
pp. 1327-1334 ◽  
Author(s):  
Ivan Srejovic ◽  
Vladimir Zivkovic ◽  
Tamara Nikolic ◽  
Nevena Jeremic ◽  
Isidora Stojic ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Rat Heart ◽  
Isolated Rat Heart ◽  
Albino Rats ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
Combined Application ◽  
Wistar Albino Rats ◽  
Isolated Rat

Considering the limited data on the role of NMDA-Rs in the cardiovascular system, the aim of the present study was to examine the effects of NMDA and DL-Hcy TLHC, alone and in combination with glycine, memantine, and ifenprodil, in the isolated rat heart. The hearts of Wistar albino rats were retrogradely perfused according to the Langendorff technique at a constant perfusion pressure. The experimental protocol for all experimental groups included the stabilization period, application of estimated substance for 5 min, followed by a washout period of 10 min. Using a sensor placed in the left ventricle, we registered the following parameters of myocardial function: dp/dtmax, dp/dtmin, SLVP, DVLP, HR; CF was measured using flowmetry). We estimated the following oxidative stress biomarkers in the coronary venous effluent using spectrophotometry: TBARS, NO2−, O2−, and H2O2. NMDA alone did not induce any change in any of the observed parameters, while DL-Hcy TLHC alone, as well as a combined application of NMDA and DL-Hcy TLHC with glycine, induced a reduction of most cardiodynamic parameters. Memantine and ifenprodil induced a reduction of cardiodynamic parameters and CF, as well as some oxidative stress biomarkers.

scholarly journals The role of hydrogen sulfide in homocysteine-induced cardiodynamic effects and oxidative stress markers in the isolated rat heart

2016 ◽  
Vol 103 (4) ◽  
pp. 428-438 ◽  
Author(s):  
M Stojanovic ◽  
V Zivkovic ◽  
I Srejovic ◽  
V Jakovljevic ◽  
N Jeremic ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Sulfide ◽  
Rat Heart ◽  
Oxidative Capacity ◽  
Left Ventricular ◽  
Isolated Rat Heart ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Isolated Rat

This study aimed to assess the role of H2S in homocysteine-induced cardiodynamic effects in the isolated rat heart. The hearts were retrogradely perfused according to the Langendorff technique. The maximum and minimum rates of pressure in the left ventricle (dp/dt max, dp/dt min), systolic and diastolic left ventricular pressures (SLVP, DLVP), heart rate (HR), and coronary flow (CF) were measured. A spectrophotometrical method was used to measure the following oxidative stress markers: index of lipid peroxidation (thiobarbituric acid reactive substances, TBARS), nitrite level (NO2−), superoxide anion radicals (O2•−), and hydrogen peroxide (H2O2) concentrations. The administration of 10 µmol/l DL-homocysteine (DL-Hcy) alone decreased dp/dt max, SLVP, and CF but did not change any oxidative stress parameters. The administration of 10 µmol/l DL-propargylglycine (DL-PAG) decreased all cardiodynamic parameters and increased the concentration of O2•−. The co-administration of DL-Hcy and DL-PAG induced a significant decrease in all estimated cardiodynamic parameters and decreased the concentration of NO2− and O2•− but increased the levels of TBARS and H2O2. Homocysteine shows a lower pro-oxidative effect in the presence of hydrogen sulfide (H2S), which indicates a potential anti-oxidative capacity of H2S.

scholarly journals ROLE OF SDF-1α/CXCR4 AXIS TO THE CARDIOPROTECTIVE EFFECT OF ISCHEMIC POST-CONDITIONING IN ISOLATED RAT HEART

2013 ◽  
Vol 61 (10) ◽  
pp. E217
Author(s):  
Jeong-Su Kim ◽  
Ju-Hyun Park ◽  
Kook-Jin Chun ◽  
Young-Ho Jang ◽  
June-Hong Kim ◽  
...  
Keyword(s):  
Rat Heart ◽  
Isolated Rat Heart ◽  
Cardioprotective Effect ◽  
Cxcr4 Axis ◽  
Isolated Rat

scholarly journals Cardioprotective Effects of 9-Hydroxyellipticine on Ischemia and Reperfusion in Isolated Rat Heart

2002 ◽  
Vol 89 (1) ◽  
pp. 21-28 ◽  
Author(s):  
Kazuhiko Saeki ◽  
Ichiro Obi ◽  
Noriko Ogiku ◽  
Munekazu Shigekawa ◽  
Toshiaki Imagawa ◽  
...  
Keyword(s):  
Rat Heart ◽  
Isolated Rat Heart ◽  
Ischemia And Reperfusion ◽  
Cardioprotective Effects ◽  
Isolated Rat

P561Acute effects of dronedarone and amiodarone on functional, morphological and oxidative stress parameters in isolated rat heart with hypertension

EP Europace ◽  
2020 ◽  
Vol 22 (Supplement_1) ◽  
Author(s):  
S Simovic ◽  
J Jeremic ◽  
G Davidovic ◽  
I Srejovic ◽  
S Mitrovic ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Rat Heart ◽  
Structural Changes ◽  
Perfusion Pressure ◽  
Coronary Perfusion Pressure ◽  
Isolated Rat Heart ◽  
Coronary Perfusion ◽  
Acute Administration ◽  
Isolated Hearts ◽  
Isolated Rat

Abstract Introduction Amiodarone represents the most widely used antiarrhythmic drug, even though it has been shown that it has negative inotropic and lusitropic effect in healthy hears. On the other hand, dronedarone reduces the risk of recurrent atrial fibrillation, but with increased early mortality related to the worsening of heart failure. However, the mechanisms responsible for these fatal outcomes remain unclear and require further examinations.  Purpose To investigate acute, direct effects of Dronedarone and Amiodarone on cardiac contractility, coronary flow and oxidative stress parameters in isolated rat heart with hypertension. Methods  The present study was carried out on 18 isolated hearts of spontaneously hypertensive Wistar Kyoto male rats (6 weeks old, bodyweight 200 ± 10 g). After isolation, all hearts were retrogradely perfused according to Langendorff technique with a gradually increment of coronary perfusion pressure (CPP from 40 to 120 cm H2O) and randomly divided into 3 groups: Control (n = 6), Amiodarone (n = 6, isolated hearts perfused with Amiodarone in dose of 3 umol), Dronedarone (n = 6, isolated hearts perfused with Dronedarone in dose of 1.8 umol). During ex vivo protocol continuously were registered cardiac contractility parameters and coronary flow, while from collected coronary venous effluent markers of oxidative stress were measured. All hearts were then fixated and stained with Hematoxylin/eosin. Results  Dronedarone severely depressed the function of all cardiodynamic parameters of the heart compared with Amiodarone or Control while Amiodarone intensified the function of the isolated rat heart with hypertension compared to Control (dp/dt max mmHg/s at coronary perfusion pressure 120cmH2O Dronedarone vs. Amiodarone vs. Control 579.733 ± 202.27 vs. 3063.65 ± 467.93 vs. 2682.88 ± 368.75; p < 0.001. dp/dt min mmHg/s 120cmH2O -352.13 ± 204.65 vs. 1960 ± 242.21 vs. -1858.83 ± 118.23; p < 0.001. SLVP mmHg at CPP 120cmH20 27.8 ± 3.46 vs. 98.95 ± 11.78 vs. 71.45 ± 7.56; p < 0.001. DLVP mmHg at CPP 120cmH2O 6.32 ± 0.49 vs. 4.83 ± 0.54 vs. 0.85 ± 0.35; p < 0.001). Acute administration of Dronedarone decreased the level of NO2- and increased the level of H2O2 , while Amiodarone heightens O2- levels (O2- nmol/min g wt at coronary perfusion pressure 120cmH2O Dronedarone vs. Amiodarone vs. Control  28.62 ± 2.54 vs. 77.3 ± 8.86 vs. 31.72 ± 4.56; p < 0.001. H2O2 nmol/min g wt at CPP 120cmH2O 92.56 ± 11.65 vs. 48.63 ± 10.11 vs. 42.84 ± 84; p < 0.001. NO2- nmol/min g wt at CPP 120cmH2O 38.61 ± 4.94 vs.  82.28 ± 5.76 vs.  64.71 ± 3.51; p < 0.001). Pathohistological, structural changes were observed in both, experimental groups. Conclusions  Acute administration of Dronedarone depresses cardiac function in isolated, working rat heart with hypertension, with decreasing the NO2- levels, increasing the level of H2O2 and enhanced structural changes when compared to Amiodarone.

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