Isolation of clinically significant microorganisms from prosthetic joint tissue using BacT/ALERT paediatric blood culture bottles compared with solid culture media and enrichment broth

Pathology ◽  
2020 ◽  
Author(s):  
Jacob Birdsall ◽  
Evanthia Tambosis ◽  
Steven Siarakas
Keyword(s):  
Blood Culture ◽  
Culture Media ◽  
Solid Culture ◽  
Joint Tissue ◽  
Enrichment Broth ◽  
Prosthetic Joint ◽  
Clinically Significant

scholarly journals Comparative Study of Three Different BACTEC Culture Media for the Detection of Bacteremia in Ambulatory and Hospitalized Children

1998 ◽  
Vol 9 (2) ◽  
pp. 77-82 ◽  
Author(s):  
Deirdre L Church ◽  
H Dele Davies ◽  
G Cadrain ◽  
Cynthia L Trevenen
Keyword(s):  
Emergency Department ◽  
Blood Culture ◽  
Culture Media ◽  
Optimal Recovery ◽  
Pediatric Care ◽  
Hospitalized Children ◽  
Anaerobic Bottle ◽  
The Common ◽  
Clinically Significant ◽  
Group D

To compare the yield of two aerobic and an anaerobic BACTEC blood culture media in detecting bacteremia in ambulatory and hospitalized care settings at a children’s hospital, a prospective cohort study was completed. Over an 18-month period, equal blood volumes (minimum of 1 mL/bottle) were inoculated into a three-bottle culture set including aerobic BACTEC NR 6A, aerobic BACTEC PEDS Plus and anaerobic NR 7A broths. Chart reviews were completed on all children with bacteremia to determine whether the isolate was clinically significant based on predefined criteria. Among 5328 evaluable blood culture sets, 323 clinically significant organisms (110 from ambulatory and 213 from hospitalized children) were isolated. MostStreptococcus pneumoniae,Haemophilusspecies, andNeisseriaorMoraxellaspecies were recovered from children attending the emergency department or out-patient clinics. Important isolates in hospitalized children included most of the staphylococci andEnterobacteriaceae, and all group D enterococci, Gram-negative nonfermentative bacilli and allCandidaspecies. Overall, significantly more isolates were detected only in the anaerobic bottle from ambulatory children (P<0.0001), including 13 of 54 (24%) patients withS pneumoniaebacteremias presenting to the emergency department. This study indicated that different BACTEC blood culture media combinations are needed in ambulatory and hospitalized pediatric care settings to ensure the optimal recovery of all types of isolates. Whereas aerobic blood culture bottles are adequate for detection of bacteremia in hospitalized children, the common occurrence of fastidious organisms mandates the need for a combined aerobic/anaerobic culture set in ambulatory pediatric care settings.

scholarly journals Shotgun-Metagenomics on Positive Blood Culture Bottles Inoculated With Prosthetic Joint Tissue: A Proof of Concept Study

2020 ◽  
Vol 11 ◽  
Author(s):  
Adriana Sanabria ◽  
Erik Hjerde ◽  
Mona Johannessen ◽  
Johanna Ericson Sollid ◽  
Gunnar Skov Simonsen ◽  
...  
Keyword(s):  
Blood Culture ◽  
Positive Blood Culture ◽  
Proof Of Concept ◽  
Shotgun Metagenomics ◽  
Joint Tissue ◽  
Concept Study ◽  
Prosthetic Joint

scholarly journals Shotgun-Metagenomics Based Prediction Of Antibiotic Resistance And Virulence Determinants In Staphylococcus Aureus From Prosthetic Joint Tissue On Blood Culture Bottles

2021 ◽  
Author(s):  
Adriana Maria Sanabria ◽  
Jessin Janice ◽  
Erik Hjerde ◽  
Gunnar Skov Simonsen ◽  
Anne-Merethe Hanssen
Keyword(s):  
Staphylococcus Aureus ◽  
Blood Culture ◽  
Virulence Genes ◽  
Sequence Data ◽  
Joint Infection ◽  
Clinical Samples ◽  
Shotgun Metagenomics ◽  
Joint Tissue ◽  
Genome Coverage ◽  
Prosthetic Joint

Abstract Shotgun-metagenomics may give valuable clinical information beyond the detection of potential pathogen(s). Identification of antimicrobial resistance (AMR), virulence genes and typing directly from clinical samples has been limited due to challenges arising from incomplete genome coverage. We assessed the performance of shotgun-metagenomics on positive blood culture bottles (n = 19) with prosthetic joint tissue for typing and prediction of AMR and virulence profiles in Staphylococcus aureus. We used different approaches to determine if sequence data from reads provides more information than from assembled contigs. Only 0.18% of total reads was derived from human DNA. Shotgun-metagenomics results and conventional method results were consistent in detecting S. aureus in all samples. AMR and known prosthetic joint infection virulence genes were predicted from S. aureus. Mean coverage depth, when predicting AMR genes was 209x. Resistance phenotypes could be explained by genes predicted in the sample in most of the cases. The choice of bioinformatic data analysis had a significant impact on the results. Read-based analysis was more accurate for pathogen identification, while contigs seemed better for AMR profiling. Our study demonstrates high genome coverage and potential for typing and prediction of AMR and virulence profiles in S. aureus from shotgun-metagenomics data.

scholarly journals Characterization of Drug-Resistant Lipid-Dependent Differentially Detectable Mycobacterium tuberculosis

2021 ◽  
Vol 10 (15) ◽  
pp. 3249
Author(s):  
Annelies W. Mesman ◽  
Seung-Hun Baek ◽  
Chuan-Chin Huang ◽  
Young-Mi Kim ◽  
Sang-Nae Cho ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Culture Media ◽  
Multidrug Resistant ◽  
Culture Conditions ◽  
Sputum Smear ◽  
Smear Microscopy ◽  
Solid Culture ◽  
Lowenstein Jensen ◽  
Genetic Mechanisms ◽  
Lipid Resuscitation

An estimated 15–20% of patients who are treated for pulmonary tuberculosis (TB) are culture-negative at the time of diagnosis. Recent work has focused on the existence of differentially detectable Mycobacterium tuberculosis (Mtb) bacilli that do not grow under routine solid culture conditions without the addition of supplementary stimuli. We identified a cohort of TB patients in Lima, Peru, in whom acid-fast bacilli could be detected by sputum smear microscopy, but from whom Mtb could not be grown in standard solid culture media. When we attempted to re-grow Mtb from the frozen sputum samples of these patients, we found that 10 out of 15 could be grown in a glycerol-poor/lipid-rich medium. These fell into the following two groups: a subset that could be regrown in glycerol after “lipid-resuscitation”, and a group that displayed a heritable glycerol-sensitive phenotype that were unable to grow in the presence of this carbon source. Notably, all of the glycerol-sensitive strains were found to be multidrug resistant. Although whole-genome sequencing of the lipid-resuscitated strains identified 20 unique mutations compared to closely related strains, no single genetic lesion could be associated with this phenotype. In summary, we found that lipid-based media effectively fostered the growth of Mtb from a series of sputum smear-positive samples that were not culturable in glycerol-based Lowenstein–Jensen or 7H9 media, which is consistent with Mtb’s known preference for non-glycolytic sources during infection. Analysis of the recovered strains demonstrated that both genetic and non-genetic mechanisms contribute to the observed differential capturability, and suggested that this phenotype may be associated with drug resistance.

scholarly journals Updated Review of Blood Culture Contamination

2006 ◽  
Vol 19 (4) ◽  
pp. 788-802 ◽  
Author(s):  
Keri K. Hall ◽  
Jason A. Lyman
Keyword(s):  
Health Care ◽  
Decision Support ◽  
Blood Culture ◽  
Infection Control ◽  
Clinical Decision ◽  
Potential Utility ◽  
Diagnostic Uncertainty ◽  
Computer Based ◽  
Clinically Significant ◽  
Care Costs

SUMMARY Blood culture contamination represents an ongoing source of frustration for clinicians and microbiologists alike. Ambiguous culture results often lead to diagnostic uncertainty in clinical management and are associated with increased health care costs due to unnecessary treatment and testing. A variety of strategies have been investigated and employed to decrease contamination rates. In addition, numerous approaches to increase our ability to distinguish between clinically significant bacteremia and contamination have been explored. In recent years, there has been an increase in the application of computer-based tools to support infection control activities as well as provide clinical decision support related to the management of infectious diseases. Finally, new approaches for estimating bacteremia risk which have the potential to decrease unnecessary blood culture utilization have been developed and evaluated. In this review, we provide an overview of blood culture contamination and describe the potential utility of a variety of approaches to improve both detection and prevention. While it is clear that progress is being made, fundamental challenges remain.

scholarly journals Advantage of combining resin with lytic BACTEC blood culture media.

1997 ◽  
Vol 35 (10) ◽  
pp. 2634-2638 ◽  
Author(s):  
P Rohner ◽  
B Pepey ◽  
R Auckenthaler
Keyword(s):  
Blood Culture ◽  
Culture Media
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