KLF1 variants and the impact on the expression of red blood cell surface molecules in blood donors with the In(Lu) phenotype

Pathology ◽  
2018 ◽  
Vol 50 ◽  
pp. S104
Author(s):  
Nicole S. Fraser ◽  
Assia Moussa ◽  
Christine M. Knauth ◽  
Elizna M. Schoeman ◽  
Catherine A. Hyland ◽  
...  
Keyword(s):  
Cell Surface ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Blood Donors ◽  
Cell Surface Molecules ◽  
Surface Molecules ◽  
The Impact

Assay of Spherical Cell Surface Molecules by Thermal Lens Microscopy and Its Application to Blood Cell Substances

2001 ◽  
Vol 73 (17) ◽  
pp. 4333-4337 ◽  
Author(s):  
Hiroko Kimura ◽  
Kazuya Sekiguchi ◽  
Takehiko Kitamori ◽  
Tsuguo Sawada ◽  
Masahiro Mukaida
Keyword(s):  
Cell Surface ◽  
Blood Cell ◽  
Thermal Lens ◽  
Spherical Cell ◽  
Cell Surface Molecules ◽  
Surface Molecules ◽  
Thermal Lens Microscopy

scholarly journals Impact of Cell Surface Molecules on Conjugative Transfer of the Integrative and Conjugative Element ICESt3ofStreptococcus thermophilus

2017 ◽  
Vol 84 (5) ◽  
Author(s):  
Narimane Dahmane ◽  
Emilie Robert ◽  
Julien Deschamps ◽  
Thierry Meylheuc ◽  
Christine Delorme ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Cell Surface ◽  
Bacterial Genome ◽  
Conjugative Transfer ◽  
Cell Surface Molecules ◽  
Content Type ◽  
Teichoic Acids ◽  
Surface Molecules ◽  
Donor Cells ◽  
The Impact

ABSTRACTIntegrative conjugative elements (ICEs) are chromosomal elements that are widely distributed in bacterial genomes, hence contributing to genome plasticity, adaptation, and evolution of bacteria. Conjugation requires a contact between both the donor and the recipient cells and thus likely depends on the composition of the cell surface envelope. In this work, we investigated the impact of different cell surface molecules, including cell surface proteins, wall teichoic acids, lipoteichoic acids, and exopolysaccharides, on the transfer and acquisition of ICESt3fromStreptococcus thermophilus. The transfer of ICESt3from wild-type (WT) donor cells to mutated recipient cells increased 5- to 400-fold when recipient cells were affected in lipoproteins, teichoic acids, or exopolysaccharides compared to when the recipient cells were WT. These mutants displayed an increased biofilm-forming ability compared to the WT, suggesting better cell interactions that could contribute to the increase of ICESt3acquisition. Microscopic observations ofS. thermophiluscell surface mutants showed different phenotypes (aggregation in particular) that can also have an impact on conjugation. In contrast, the same mutations did not have the same impact when the donor cells, instead of recipient cells, were mutated. In that case, the transfer frequency of ICESt3decreased compared to that with the WT. The same observation was made when both donor and recipient cells were mutated. The dominant effect of mutations in donor cells suggests that modifications of the cell envelope could impair the establishment or activity of the conjugation machinery required for DNA transport.IMPORTANCEICEs contribute to horizontal gene transfer of adaptive traits (for example, virulence, antibiotic resistance, or biofilm formation) and play a considerable role in bacterial genome evolution, thus underlining the need of a better understanding of their conjugative mechanism of transfer. While most studies focus on the different functions encoded by ICEs, little is known about the effect of host factors on their conjugative transfer. Using ICESt3ofS. thermophilusas a model, we demonstrated the impact of lipoproteins, teichoic acids, and exopolysaccharides on ICE transfer and acquisition. This opens up new avenues to control gene transfer mediated by ICEs.

scholarly journals Cladribine Alters Immune Cell Surface Molecules for Adhesion and Costimulation: Further Insights to the Mode of Action in Multiple Sclerosis

Cells ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 3116
Author(s):  
Tobias Moser ◽  
Lena Hoepner ◽  
Kerstin Schwenker ◽  
Michael Seiberl ◽  
Julia Feige ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
T Cells ◽  
B Cells ◽  
Cell Surface ◽  
Mode Of Action ◽  
Immune Cell ◽  
Cell Surface Molecules ◽  
Surface Molecules ◽  
Surface Patterns ◽  
The Impact

Cladribine (CLAD) is a deoxyadenosine analogue prodrug which is given in multiple sclerosis (MS) as two short oral treatment courses 12 months apart. Reconstitution of adaptive immune function following selective immune cell depletion is the presumed mode of action. In this exploratory study, we investigated the impact of CLAD tablets on immune cell surface molecules for adhesion (CAMs) and costimulation (CoSs) in people with MS (pwMS). We studied 18 pwMS who started treatment with CLAD and 10 healthy controls (HCs). Peripheral blood mononuclear cells were collected at baseline and every 3 months throughout a 24-month period. We analysed ICAM-1, LFA-1, CD28, HLADR, CD154, CD44, VLA-4 (CD49d/CD29), PSGL-1 and PD-1 with regard to their expression on B and T cells (T helper (Th) and cytotoxic T cells (cT)) and surface density (mean fluorescence intensity, MFI) by flow cytometry. The targeted analysis of CAM and CoS on the surface of immune cells in pwMS revealed a higher percentage of ICAM-1 (B cells, Th, cT), LFA-1 (B cells, cT), HLADR (B cells, cT), CD28 (cT) and CD154 (Th). In pwMS, we found lower frequencies of Th and cT cells expressing PSGL-1 and B cells for the inhibitory signal PD-1, whereas the surface expression of LFA-1 on cT and of HLADR on B cells was denser. Twenty-four months after the first CLAD cycle, the frequencies of B cells expressing CD44, CD29 and CD49d were lower compared with the baseline, together with decreased densities of ICAM-1, CD44 and HLADR. The rate of CD154 expressing Th cells dropped at 12 months. For cT, no changes were seen for frequency or density. Immune reconstitution by oral CLAD was associated with modification of the pro-migratory and -inflammatory surface patterns of CAMs and CoSs in immune cell subsets. This observation pertains primarily to B cells, which are key cells underlying MS pathogenesis.

scholarly journals Cell surface molecules that bind fibronectin's matrix assembly domain

1991 ◽  
Vol 266 (15) ◽  
pp. 9697-9702 ◽  
Author(s):  
A.H. Limper ◽  
B.J. Quade ◽  
R.M. LaChance ◽  
T.M. Birkenmeier ◽  
T.S. Rangwala ◽  
...  
Keyword(s):  
Cell Surface ◽  
Cell Surface Molecules ◽  
Matrix Assembly ◽  
Surface Molecules

Cell surface molecules (NCAM and LI) in intrastriatal transplants of embryonic mesencephalon in rats

Neuroscience ◽  
1996 ◽  
Vol 73 (1) ◽  
pp. 161-169 ◽  
Author(s):  
G. Gopinath ◽  
V. Sable ◽  
K. Sailaja ◽  
P.N. Tandon
Keyword(s):  
Cell Surface ◽  
Cell Surface Molecules ◽  
Surface Molecules

Expression profiles of novel cell surface molecules on B-cell subsets and plasma cells as analyzed by flow cytometry

2011 ◽  
Vol 134 (2) ◽  
pp. 113-121 ◽  
Author(s):  
Laia Llinàs ◽  
Adriana Lázaro ◽  
Jose de Salort ◽  
Jessica Matesanz-Isabel ◽  
Jordi Sintes ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Cell Surface ◽  
B Cell ◽  
Plasma Cells ◽  
Expression Profiles ◽  
Cell Surface Molecules ◽  
Surface Molecules ◽  
B Cell Subsets
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