Integration of ISO15189 and external quality assurance data to assist the detection of poor laboratory performance in New South Wales

Pathology ◽  
2018 ◽  
Vol 50 ◽  
pp. S92
Author(s):  
Brett A. Lidbury ◽  
Gus Koerbin ◽  
Alice M. Richardson ◽  
Tony Badrick
Keyword(s):  
Quality Assurance ◽  
New South ◽  
New South Wales ◽  
External Quality Assurance ◽  
External Quality ◽  
Laboratory Performance ◽  
South Wales

Bivariate statistical approach to evaluate laboratory performance by analysis of standard curves in an External Quality Assurance program for quantitative assays based on real-time PCR with Taq-Man™ probes

2006 ◽  
Vol 44 (1) ◽  
Author(s):  
Giuseppe Reina ◽  
Claudio Orlando ◽  
Paola Rebora ◽  
Federico Ambrogi ◽  
Claudia Casini Raggi ◽  
...  
Keyword(s):  
Quality Assurance ◽  
Real Time ◽  
Real Time Pcr ◽  
Statistical Approach ◽  
External Quality Assurance ◽  
Quality Assurance Program ◽  
Analytical Sensitivity ◽  
External Quality ◽  
Laboratory Performance ◽  
The Impact

AbstractRecently a revolutionary technique for quantitative PCR determination was introduced in diagnostic laboratories. To determine the influence of technical variability on the reliability of the quantitative assay, it is crucial to use External Quality Assurance (EQA) programs. An EQA program was developed in Italy to check the analytical performance of real-time PCR procedures based on Taq-Man™ probes. This article suggests a new statistical approach to discriminate, using a bivariate technique, laboratory performance that appears to be questionable, by separately considering the two main features of the standard curve: analytical sensitivity and efficiency. Furthermore, specific indexes to evaluate the impact of these two features on the determination of the initial number of molecules are given to help to improve the assay procedure.

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