Development of an in-house real-time pcr to monitor the prevalence of macrolide-resistant Mycoplasma pneumoniae in Hong Kong

Pathology ◽  
2017 ◽  
Vol 49 ◽  
pp. S116
Author(s):  
Ho-Yin Lam ◽  
Thomas Hin-Ching Wong ◽  
Anthony Tsz-Chun Wong ◽  
Gilman Kit-Hang Siu ◽  
Wing-Cheong Yam ◽  
...  
Keyword(s):  
Hong Kong ◽  
Real Time ◽  
Mycoplasma Pneumoniae ◽  
Real Time Pcr

scholarly journals Sensitive Detection of Mycoplasma pneumoniae in Human Respiratory Tract Samples by Optimized Real-Time PCR Approach

2007 ◽  
Vol 45 (8) ◽  
pp. 2726-2730 ◽  
Author(s):  
R. Dumke ◽  
N. Schurwanz ◽  
M. Lenz ◽  
M. Schuppler ◽  
C. Luck ◽  
...  
Keyword(s):  
Respiratory Tract ◽  
Real Time ◽  
Mycoplasma Pneumoniae ◽  
Real Time Pcr ◽  
Sensitive Detection ◽  
Human Respiratory Tract

scholarly journals Comparison and Evaluation of Real-Time PCR, Real-Time Nucleic Acid Sequence-Based Amplification, Conventional PCR, and Serology for Diagnosis of Mycoplasma pneumoniae

2003 ◽  
Vol 41 (9) ◽  
pp. 4366-4371 ◽  
Author(s):  
K. E. Templeton ◽  
S. A. Scheltinga ◽  
A. W. Graffelman ◽  
J. M. van Schie ◽  
J. W. Crielaard ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Mycoplasma Pneumoniae ◽  
Real Time Pcr ◽  
Nucleic Acid Sequence ◽  
Conventional Pcr

Detection of Mycoplasma pneumoniae in respiratory samples by real-time PCR using an inhibition control

2003 ◽  
Vol 55 (1) ◽  
pp. 149-153 ◽  
Author(s):  
Dominique Ursi ◽  
Kristien Dirven ◽  
Katherine Loens ◽  
Margareta Ieven ◽  
Herman Goossens
Keyword(s):  
Real Time ◽  
Mycoplasma Pneumoniae ◽  
Real Time Pcr ◽  
Inhibition Control

scholarly journals Real-time detection of Mycoplasma pneumoniae in respiratory samples with an internal processing control

2006 ◽  
Vol 55 (2) ◽  
pp. 149-155 ◽  
Author(s):  
David Pitcher ◽  
Victoria J. Chalker ◽  
Carmen Sheppard ◽  
Robert C. George ◽  
Timothy G. Harrison
Keyword(s):  
Real Time ◽  
Mycoplasma Pneumoniae ◽  
Real Time Pcr ◽  
Bacterial Species ◽  
Batch Processing ◽  
Clinical Samples ◽  
Clinical Screening ◽  
Signal Production ◽  
Processing Control ◽  
Internal Processing

Real-time PCR was employed to detect a region of the P1 cytadhesin gene of Mycoplasma pneumoniae in clinical samples. An internal processing control was included that could be co-amplified simultaneously in the same reaction tube. The assay could reproducibly detect 1×103 M. pneumoniae organisms ml−1 in clinical samples. There was no amplification of DNA or signal production from 15 other species of human mycoplasmas and 19 other bacterial species. Using a panel of 175 respiratory samples taken from patients with pneumonia of proven aetiology, the sensitivity was found to be 60 % and the specificity of the assay 96·7 % when compared with serology. This assay is suitable for same-day diagnosis of M. pneumoniae infection and batch processing of respiratory samples for clinical screening.

Diagnostic sensitivity of a rapid antigen test for the detection of Mycoplasma pneumoniae: Comparison with real-time PCR

2015 ◽  
Vol 21 (6) ◽  
pp. 473-475 ◽  
Author(s):  
Naoyuki Miyashita ◽  
Yasuhiro Kawai ◽  
Takaaki Tanaka ◽  
Hiroto Akaike ◽  
Hideto Teranishi ◽  
...  
Keyword(s):  
Real Time ◽  
Mycoplasma Pneumoniae ◽  
Real Time Pcr ◽  
Diagnostic Sensitivity ◽  
Antigen Test
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