Hypoxia-inducible factor-1 up-regulates the expression of Toll-like receptor 4 in pancreatic cancer cells under hypoxic conditions

Pancreatology ◽  
2012 ◽  
Vol 12 (2) ◽  
pp. 170-178 ◽  
Author(s):  
Ping Fan ◽  
Jian-Jun Zhang ◽  
Bo Wang ◽  
Han-Qing Wu ◽  
Si-Xing Zhou ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Cancer Cells ◽  
Hypoxia Inducible Factor ◽  
Toll Like Receptor ◽  
Toll Like Receptor 4 ◽  
Hypoxia Inducible Factor 1 ◽  
Hypoxic Conditions ◽  
Pancreatic Cancer Cells

scholarly journals Hypoxia Inducible Factor-1 Mediates Effects of Insulin on Pancreatic Cancer Cells and Disturbs Host Energy Homeostasis

2007 ◽  
Vol 170 (2) ◽  
pp. 469-477 ◽  
Author(s):  
Feng Wang ◽  
Shu Shun Li ◽  
Ralf Segersvärd ◽  
Lisa Strömmer ◽  
Karl-Gösta Sundqvist ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Cancer Cells ◽  
Energy Homeostasis ◽  
Hypoxia Inducible Factor ◽  
Hypoxia Inducible Factor 1 ◽  
Pancreatic Cancer Cells

scholarly journals Investigation of The Effects of The Toll-Like Receptor 4 Pathway on Immune Checkpoint Vista in Pancreatic Cancer

2021 ◽  
Author(s):  
Kübra Sena Bas Topcu ◽  
Emine Nedime KORUCU ◽  
Esma Menevse ◽  
Nadir Kocak ◽  
Tugce Duran
Keyword(s):  
Gene Expression ◽  
Pancreatic Cancer ◽  
Cancer Cells ◽  
T Cell Activation ◽  
Immune Checkpoint ◽  
Signal Molecules ◽  
Check Point ◽  
Toll Like Receptor ◽  
Toll Like Receptor 4 ◽  
Pancreatic Cancer Cells

Abstract Pancreatic ductal adenocarcinoma (PDAC) is one of the most common malignant tumors of the pancreas. Preclinical studies show that it evades the immune system with immune checkpoints and promotes tumor development. V-domain Ig suppressor of T cell activation (VISTA) is a new immune-check point from the B7 family and is highly expressed in cancer cells. Overexpression of toll like receptor 4 (TLR4) in pancreatic adenocarcinoma is associated with inducing tumorigenesis, tumor growth and resistance to chemotherapy. Naloxone is an opioid and inhibits TLR4-ligand association. In this study, we investigated the connection of TLR4 and downstream pathway with immune-check point VISTA in pancreatic cancer proliferation. We first collected pancreatic cancer-related datasets using the GEPIA2 and UALCAN databases. Based on the data obtained the effect of different concentrations and incubation times of Naloxone were used on PANC-1 cells proliferation. Combination of Naloxone and VISTA-siRNA were applied, and effect of both Naloxone and combined reatment on TLR4, Interleukin 1 receptor associated kinase 4 (IRAK4) and VISTA gene expression were analysed in pancreatic cancer cells. As a result of analysis with Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR), gene expression levels of TLR4, IRAK4 and VISTA were significantly suppressed and cell proliferation was significantly reduced. We found that administration of Naloxone and VISTA-siRNA in combination to PDAC cells suppressed signaling. Therefore, we considered that the relationship between VISTA and TLR4 signaling pathways and the other possible associated signal molecules may be an important marker in determining the response of immune checkpoint inhibitors in cancer treatment.

scholarly journals ER stress compromises VEGFA secretion of pancreatic cancer cells under hypoxic conditions, thereby contributing to tumor hypoxia

HPB ◽  
2016 ◽  
Vol 18 ◽  
pp. e837
Author(s):  
B. Kong ◽  
T. Cheng ◽  
I. Regel ◽  
S. Raulefs ◽  
H. Friess ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Er Stress ◽  
Cancer Cells ◽  
Tumor Hypoxia ◽  
Hypoxic Conditions ◽  
Pancreatic Cancer Cells

Hypoxia inducible factor (HIF)-1α directly activates leptin receptor (Ob-R) in pancreatic cancer cells

2014 ◽  
Vol 354 (1) ◽  
pp. 172-180 ◽  
Author(s):  
He Ren ◽  
Lingling Jia ◽  
Tiansuo Zhao ◽  
Huan Zhang ◽  
Jing Chen ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Cancer Cells ◽  
Leptin Receptor ◽  
Hypoxia Inducible Factor ◽  
Pancreatic Cancer Cells
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