R123: Gene Expression Profiles in Ex Vivo Model of Nasal Polyp

2006 ◽  
Vol 135 (2_suppl) ◽  
pp. P147-P147
Author(s):  
Kyung-Wook Heo ◽  
Seong-Kook Park
Keyword(s):  
Gene Expression ◽  
Nasal Polyp ◽  
Ex Vivo ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Ex Vivo Model

Magnesium-Enriched Hydroxyapatite Versus Autologous Bone in Maxillary Sinus Grafting: Combining Histomorphometry With Osteoblast Gene Expression Profiles Ex Vivo

2009 ◽  
Vol 80 (4) ◽  
pp. 586-593 ◽  
Author(s):  
Roberto Crespi ◽  
Elisabetta Mariani ◽  
Elisa Benasciutti ◽  
Paolo Capparè ◽  
Simone Cenci ◽  
...  
Keyword(s):  
Gene Expression ◽  
Maxillary Sinus ◽  
Ex Vivo ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Autologous Bone ◽  
Sinus Grafting

scholarly journals Harnessing Gene Expression Profiles for the Identification of Ex Vivo Drug Response Genes in Pediatric Acute Myeloid Leukemia

Cancers ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 1247 ◽  
Author(s):  
David G.J. Cucchi ◽  
Costa Bachas ◽  
Marry M. van den Heuvel-Eibrink ◽  
Susan T.C.J.M. Arentsen-Peters ◽  
Zinia J. Kwidama ◽  
...  
Keyword(s):  
Gene Expression ◽  
Drug Resistance ◽  
Drug Response ◽  
Molecular Mechanisms ◽  
Ex Vivo ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Leukemic Cells ◽  
Treatment Optimization ◽  
Pediatric Aml

Novel treatment strategies are of paramount importance to improve clinical outcomes in pediatric AML. Since chemotherapy is likely to remain the cornerstone of curative treatment of AML, insights in the molecular mechanisms that determine its cytotoxic effects could aid further treatment optimization. To assess which genes and pathways are implicated in tumor drug resistance, we correlated ex vivo drug response data to genome-wide gene expression profiles of 73 primary pediatric AML samples obtained at initial diagnosis. Ex vivo response of primary AML blasts towards cytarabine (Ara C), daunorubicin (DNR), etoposide (VP16), and cladribine (2-CdA) was associated with the expression of 101, 345, 206, and 599 genes, respectively (p < 0.001, FDR 0.004–0.416). Microarray based expression of multiple genes was technically validated using qRT-PCR for a selection of genes. Moreover, expression levels of BRE, HIF1A, and CLEC7A were confirmed to be significantly (p < 0.05) associated with ex vivo drug response in an independent set of 48 primary pediatric AML patients. We present unique data that addresses transcriptomic analyses of the mechanisms underlying ex vivo drug response of primary tumor samples. Our data suggest that distinct gene expression profiles are associated with ex vivo drug response, and may confer a priori drug resistance in leukemic cells. The described associations represent a fundament for the development of interventions to overcome drug resistance in AML, and maximize the benefits of current chemotherapy for sensitive patients.

scholarly journals Identification of Tissue-Restricted Transcripts in Human Islets

Endocrinology ◽  
2004 ◽  
Vol 145 (10) ◽  
pp. 4513-4521 ◽  
Author(s):  
Antonella Maffei ◽  
Zhuoru Liu ◽  
Piotr Witkowski ◽  
Federica Moschella ◽  
Giovanna Del Pozzo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Islet Cell ◽  
Ex Vivo ◽  
Expression Profiles ◽  
Kidney Tissue ◽  
Gene Expression Profiles ◽  
Human Islets ◽  
Gene Products ◽  
Associated Proteins

Abstract The purpose of our study was to identify transcripts specific for tissue-restricted, membrane-associated proteins in human islets that, in turn, might serve as markers of healthy or diseased islet cell masses. Using oligonucleotide chips, we obtained gene expression profiles of human islets for comparison with the profiles of exocrine pancreas, liver, and kidney tissue. As periislet presence of type 1 interferon is associated with the development of type 1 diabetes, the expression profile of human islets treated ex vivo with interferon-α2β (IFNα2β) was also determined. A set of genes encoding transmembrane- or membrane-associated proteins with novel islet-restricted expression was resolved by determining the intersection of the islet set with the complement of datasets obtained from other tissues. Under the influence of IFNα2β, the expression levels of transcripts for several of the identified gene products were up- or down-regulated. One of the islet-restricted gene products identified in this study, vesicular monoamine transporter type 2, was shown to bind [3H]dihydrotetrabenazine, a ligand with derivatives suitable for positron emission tomography imaging. We report here the first comparison of gene expression profiles of human islets with other tissues and the identification of a target molecule with possible use in determining islet cell masses.

scholarly journals Effect ofEx VivoCulture Conditions on Immunosuppression by Human Mesenchymal Stem Cells

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Myoung Woo Lee ◽  
Dae Seong Kim ◽  
Somi Ryu ◽  
In Keun Jang ◽  
Hye Jin Kim ◽  
...  
Keyword(s):  
Gene Expression ◽  
Ex Vivo ◽  
Expression Profiles ◽  
Human Mesenchymal Stem Cells ◽  
Gene Expression Profiles ◽  
Culture Conditions ◽  
Seeding Density ◽  
Initial Seeding ◽  
Density Treatment

A microarray analysis was performed to investigate whetherex vivoculture conditions affect the characteristics of MSCs. Gene expression profiles were mainly influenced by the level of cell confluence rather than initial seeding density. The analysis showed that 276 genes were upregulated and 230 genes downregulated in MSCs harvested at~90% versus~50% confluence (P<0.05,FC>2). The genes that were highly expressed in MSCs largely corresponded to chemotaxis, inflammation, and immune responses, indicating direct or indirect involvement in immunomodulatory functions. Specifically, PTGES and ULBP1 were up-regulated in MSCs harvested at high density. Treatment of MSCs withPTGESorULBP1siRNA reversed their inhibition of T-cell proliferationin vitro. The culture conditions such as cell confluence at harvest seem to be important for gene expression profile of MSCs; therefore, the results of this study may provide useful guidelines for the harvest of MSCs that can appropriately suppress the immune response.

scholarly journals Gene expression in mouse ovarian follicle development in vivo versus an ex vivo alginate culture system

Reproduction ◽  
2011 ◽  
Vol 142 (2) ◽  
pp. 309-318 ◽  
Author(s):  
Elizabeth M Parrish ◽  
Anaar Siletz ◽  
Min Xu ◽  
Teresa K Woodruff ◽  
Lonnie D Shea
Keyword(s):  
Gene Expression ◽  
Transforming Growth Factor ◽  
Ex Vivo ◽  
Expression Profiles ◽  
Ovarian Follicle ◽  
Gene Expression Profiles ◽  
Follicle Development ◽  
Α Subunit

Ovarian follicle maturation results from a complex interplay of endocrine, paracrine, and direct cell–cell interactions. This study compared the dynamic expression of key developmental genes during folliculogenesis in vivo and during in vitro culture in a 3D alginate hydrogel system. Candidate gene expression profiles were measured within mouse two-layered secondary follicles, multi-layered secondary follicles, and cumulus–oocyte complexes (COCs). The expression of 20 genes involved in endocrine communication, growth signaling, and oocyte development was investigated by real-time PCR. Gene product levels were compared between i) follicles of similar stage and ii) COCs derived either in vivo or by in vitro culture. For follicles cultured for 4 days, the expression pattern and the expression level of 12 genes were the same in vivo and in vitro. Some endocrine (cytochrome P450, family 19, subfamily A, polypeptide 1 (Cyp19a1) and inhibin βA subunit (Inhba)) and growth-related genes (bone morphogenetic protein 15 (Bmp15), kit ligand (Kitl), and transforming growth factor β receptor 2 (Tgfbr2)) were downregulated relative to in vivo follicles. For COCs obtained from cultured follicles, endocrine-related genes (inhibin α-subunit (Inha) and Inhba) had increased expression relative to in vivo counterparts, whereas growth-related genes (Bmp15, growth differentiation factor 9, and kit oncogene (Kit)) and zona pellucida genes were decreased. However, most of the oocyte-specific genes (e.g. factor in the germline α (Figla), jagged 1 (Jag1), and Nlrp5 (Mater)) were expressed in vitro at the same level and with the same pattern as in vivo-derived follicles. These studies establish the similarities and differences between in vivo and in vitro cultured follicles, guiding the creation of environments that maximize follicle development and oocyte quality.

Gene expression profiles in human nasal polyp tissues studied by means of DNA microarray

2004 ◽  
Vol 114 (4) ◽  
pp. 783-790 ◽  
Author(s):  
Zheng Liu ◽  
Jean Kim ◽  
Joseph P. Sypek ◽  
I-Ming Wang ◽  
Heidi Horton ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dna Microarray ◽  
Nasal Polyp ◽  
Expression Profiles ◽  
Gene Expression Profiles

scholarly journals Adult and neonatal astrocytes exhibit diverse gene expression profiles in response to beta amyloid <i>ex vivo</i>

2012 ◽  
Vol 02 (02) ◽  
pp. 57-67 ◽  
Author(s):  
Antti Kurronen ◽  
Rea Pihlaja ◽  
Eveliina Pollari ◽  
Katja Kanninen ◽  
Markus Storvik ◽  
...  
Keyword(s):  
Gene Expression ◽  
Ex Vivo ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Beta Amyloid

Shifting Gene Expression Profiles During Ex Vivo Culture of Renal Tumor Cells: Implications for Cancer Immunotherapy

2003 ◽  
Vol 14 (3) ◽  
pp. 133-145 ◽  
Author(s):  
Federica Moschella ◽  
Richard P. Catanzaro ◽  
Brygida Bisikirska ◽  
Ihor S. Sawczuk ◽  
Kyriakos P. Papadapoulos ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cancer Immunotherapy ◽  
Tumor Cells ◽  
Renal Tumor ◽  
Ex Vivo ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Ex Vivo Culture
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