Analysis of aberrations and performance evaluation of adaptive optics in two-photon light-sheet microscopy

2019 ◽  
Vol 435 ◽  
pp. 46-53 ◽  
Author(s):  
Caihua Zhang ◽  
Wenqiang Sun ◽  
Quanquan Mu ◽  
Zhaoliang Cao ◽  
Xingyun Zhang ◽  
...  
Keyword(s):  
Performance Evaluation ◽  
Adaptive Optics ◽  
Light Sheet ◽  
Two Photon ◽  
Light Sheet Microscopy ◽  
And Performance

scholarly journals Characterization of Scattering Effects in Phantom Samples using Single and Two-Photon Excitation Light Sheet Microscopy

2012 ◽  
Vol 102 (3) ◽  
pp. 195a-196a
Author(s):  
Zeno Lavagnino ◽  
Francesca Cella Zanacchi ◽  
Emiliano Ronzitti ◽  
Ivan Coto Hernandez ◽  
Alberto Diaspro
Keyword(s):  
Light Sheet ◽  
Excitation Light ◽  
Two Photon ◽  
Light Sheet Microscopy ◽  
Photon Excitation ◽  
Scattering Effects ◽  
Two Photon Excitation

scholarly journals Wide field intravital imaging by two-photon-excitation digital-scanned light-sheet microscopy (2p-DSLM) with a high-pulse energy laser

2014 ◽  
Vol 5 (10) ◽  
pp. 3311 ◽  
Author(s):  
Atsushi Maruyama ◽  
Yusuke Oshima ◽  
Hiroko Kajiura-Kobayashi ◽  
Shigenori Nonaka ◽  
Takeshi Imamura ◽  
...  
Keyword(s):  
Pulse Energy ◽  
Light Sheet ◽  
Intravital Imaging ◽  
Wide Field ◽  
High Pulse Energy ◽  
Two Photon ◽  
Light Sheet Microscopy ◽  
Photon Excitation ◽  
High Pulse ◽  
Energy Laser

scholarly journals Light-sheet microscopy in thick media using scanned Bessel beams and two-photon fluorescence excitation

2013 ◽  
Vol 21 (11) ◽  
pp. 13824 ◽  
Author(s):  
Florian O. Fahrbach ◽  
Vasily Gurchenkov ◽  
Kevin Alessandri ◽  
Pierre Nassoy ◽  
Alexander Rohrbach
Keyword(s):  
Light Sheet ◽  
Bessel Beams ◽  
Fluorescence Excitation ◽  
Two Photon ◽  
Light Sheet Microscopy ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

Two-photon three-axis digital scanned light-sheet microscopy (2P3A-DSLM)

CLEO: 2014 ◽  
2014 ◽  
Author(s):  
Weijian Zong ◽  
Xuanyang Chen ◽  
Jia Zhao ◽  
Yunfeng Zhang ◽  
Ming Fan ◽  
...  
Keyword(s):  
Light Sheet ◽  
Two Photon ◽  
Light Sheet Microscopy

scholarly journals Dual-view light-sheet imaging through tilted glass interface using a deformable mirror

2020 ◽  
Author(s):  
N Vladimirov ◽  
F Preusser ◽  
J Wisniewski ◽  
Z Yaniv ◽  
RA Desai ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
High Speed ◽  
Microfluidic Channel ◽  
Microfluidic Devices ◽  
Light Sheet ◽  
Stochastic Gradient Descent ◽  
Deformable Mirror ◽  
Optical Aberrations ◽  
Light Sheet Microscopy ◽  
Gradient Descent Algorithm

AbstractLight-sheet microscopy has become one of the primary tools for imaging live developing organisms because of its high speed, low phototoxicity, and optical sectioning capabilities. Detection from multiple sides (multi-view imaging) additionally allows nearly isotropic resolution via computational merging of the views. However, conventional light-sheet microscopes require that the sample is suspended in a gel to allow optical access from two or more sides. At the same time, the use of microfluidic devices is highly desirable for many experiments, but geometric constrains and strong optical aberrations caused by the coverslip titled relative to objectives make the use of multi-view lightsheet challenging for microfluidics.In this paper we describe the use of adaptive optics (AO) to enable multi-view light-sheet microscopy in such microfluidic setup by correcting optical aberrations introduced by the tilted coverslip. The optimal shape of deformable mirror is computed by an iterative stochastic gradient-descent algorithm that optimizes PSF in two orthogonal planes simultaneously. Simultaneous AO correction in two optical arms is achieved via a knife-edge mirror that splits excitation path and combines the detection path.We characterize the performance of this novel microscope setup and, by dual-view light-sheet imaging of C.elegans inside a microfluidic channel, demonstrate a drastic improvement of image quality due to AO and dual-view reconstruction. Our microscope design allows multi-view light-sheet microscopy with microfluidic devices for precisely controlled experimental conditions and high-content screening.

scholarly journals Multiview Deconvolution Two-photon Laser Scanning Microscopy

2020 ◽  
Author(s):  
Dimitrios Kapsokalyvas ◽  
Rodrigo Rosas ◽  
Rob Janssen ◽  
Jo Vanoevelen ◽  
Martin Strauch ◽  
...  
Keyword(s):  
Second Harmonic ◽  
Light Sheet ◽  
Laser Scanning Microscopy ◽  
Three Dimensions ◽  
Scanning Microscopy ◽  
Fluorescence Signal ◽  
Microscopy Imaging ◽  
Two Photon ◽  
Light Sheet Microscopy ◽  
3D Image Reconstruction

Abstract Imaging in three dimensions is necessary for thick tissues and small organisms. This is possible with tomographic optical microscopy techniques such as confocal, two-photon and light sheet microscopy. All these techniques suffer from anisotropic resolution and limited penetration depth. In the past, Multiview microscopy - imaging the sample from different angles followed by 3D image reconstruction - was developed to address this issue for light sheet microscopy based on fluorescence signal. In this study we applied this methodology to accomplish Multiview imaging with two-photon microscopy based on fluorescence and additionally second harmonic signal from myosin and collagen. It was shown that isotropic resolution was achieved, the entirety of the sample was visualized, and interference artifacts were suppressed allowing clear visualization of collagen fibrils and myofibrils. This method can be applied to any scanning microscopy technique without microscope modifications. It can be used for imaging tissue and whole mount small organisms such as heart tissue, and zebrafish larva in 3D, label-free or stained, with at least 3-fold axial resolution improvement which can be significant for the accurate quantification of small 3D structures.

scholarly journals Observing the Cell in Its Native State: Imaging Subcellular Dynamics in Multicellular Organisms

2018 ◽  
Author(s):  
Tsung-Li Liu ◽  
Srigokul Upadhyayula ◽  
Daniel E. Milkie ◽  
Ved Singh ◽  
Kai Wang ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
High Speed ◽  
Developmental Stages ◽  
Phenotypic Diversity ◽  
Metastatic Cancer ◽  
Light Sheet ◽  
Intrinsic Variability ◽  
Spatiotemporal Resolution ◽  
Light Sheet Microscopy

AbstractTrue physiological imaging of subcellular dynamics requires studying cells within their parent organisms, where all the environmental cues that drive gene expression, and hence the phenotypes we actually observe, are present. A complete understanding also requires volumetric imaging of the cell and its surroundings at high spatiotemporal resolution without inducing undue stress on either. We combined lattice light sheet microscopy with two-channel adaptive optics to achieve, across large multicellular volumes, noninvasive aberration-free imaging of subcellular processes, including endocytosis, organelle remodeling during mitosis, and the migration of axons, immune cells, and metastatic cancer cells in vivo. The technology reveals the phenotypic diversity within cells across different organisms and developmental stages, and may offer insights into how cells harness their intrinsic variability to adapt to different physiological environments.One Sentence SummaryCombining lattice light sheet microscopy with adaptive optics enables high speed, high resolution in vivo 3D imaging of dynamic processes inside cells under physiological conditions within their parent organisms.

Adaptive optics light-sheet microscopy for functional neuroimaging

2021 ◽  
Author(s):  
Antoine Hubert ◽  
Fabrice Harms ◽  
Sophie Imperato ◽  
Vincent Loriette ◽  
Cynthia Veilly ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
Functional Neuroimaging ◽  
Light Sheet ◽  
Light Sheet Microscopy
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