Background suppression in confocal scanning fluorescence microscopy with superoscillations

2018 ◽  
Vol 426 ◽  
pp. 541-546 ◽  
Author(s):  
Vannhu Le ◽  
Xiaona Wang ◽  
Cuifang Kuang ◽  
Xu Liu
Keyword(s):  
Fluorescence Microscopy ◽  
Background Suppression ◽  
Confocal Scanning

In vivo vascular imaging with adaptive optics confocal scanning fluorescence microscopy

2014 ◽  
Author(s):  
Zhibin Wang ◽  
Lin Wei ◽  
Yi He ◽  
Xiqi Li ◽  
Guohua Shi ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Adaptive Optics ◽  
Vascular Imaging ◽  
Confocal Scanning

Adaptive aberration correction in confocal scanning fluorescence microscopy

2014 ◽  
Vol 12 (s1) ◽  
pp. S11103-311105
Author(s):  
Zhibin Wang Zhibin Wang ◽  
Guohua Shi Guohua Shi ◽  
Yudong Zhang Yudong Zhang
Keyword(s):  
Fluorescence Microscopy ◽  
Aberration Correction ◽  
Confocal Scanning

Three-Dimensional Imaging by Confocal Scanning Fluorescence Microscopy

1986 ◽  
Vol 483 (1 Recent Advanc) ◽  
pp. 405-415 ◽  
Author(s):  
G. J. BRAKENHOFF ◽  
H. T. M. Van Der VOORT ◽  
E. A. Van SPRONSEN ◽  
N. NANNINGA
Keyword(s):  
Fluorescence Microscopy ◽  
Three Dimensional ◽  
Three Dimensional Imaging ◽  
Confocal Scanning

scholarly journals Study of interaction of antimutagenic 1,4-dihydropyridine AV-153-Na with DNA-damaging molecules and its impact on DNA repair activity

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4609 ◽  
Author(s):  
Elina Leonova ◽  
Evita Rostoka ◽  
Sylvie Sauvaigo ◽  
Larisa Baumane ◽  
Turs Selga ◽  
...  
Keyword(s):  
Dna Repair ◽  
Fluorescence Microscopy ◽  
Hydroxyl Radical ◽  
Uv Spectroscopy ◽  
Radical Scavenging ◽  
Intracellular Distribution ◽  
Dna Repair Enzymes ◽  
Abasic Sites ◽  
Repair Enzymes ◽  
Confocal Scanning

Background1,4-dihydropyridines (1,4-DHP) possesses important biochemical and pharmacological properties, including antioxidant and antimutagenic activities. It was shown that the antimutagenic 1,4-dihydropyridine AV-153-Na interacts with DNA. The aim of the current study was to test the capability of the compound to scavenge peroxynitrite and hydroxyl radical, to test intracellular distribution of the compound, and to assess the ability of the compound to modify the activity of DNA repair enzymes and to protect the DNA in living cells against peroxynitrite-induced damage.MethodsPeroxynitrite decomposition was assayed by UV spectroscopy, hydroxyl radical scavenging—by EPR spectroscopy. DNA breakage was determined by the “comet method”, activity of DNA repair enzymes—using Glyco-SPOT and ExSy-SPOT assays. Intracellular distribution of the compound was studied by laser confocal scanning fluorescence microscopy. Fluorescence spectroscopy titration and circular dichroism spectroscopy were used to study interactions of the compound with human serum albumin.ResultsSome ability to scavenge hydroxyl radical by AV-153-Na was detected by the EPR method, but it turned out to be incapable of reacting chemically with peroxynitrite. However, AV-153-Na effectively decreased DNA damage produced by peroxynitrite in cultured HeLa cells. The Glyco-SPOT test essentially revealed an inhibition by AV-153-Na of the enzymes involved thymine glycol repair. Results with ExSy-SPOT chip indicate that AV-153-Na significantly stimulates excision/synthesis repair of 8-oxoguanine (8-oxoG), abasic sites (AP sites) and alkylated bases. Laser confocal scanning fluorescence microscopy demonstrated that within the cells AV-153-Na was found mostly in the cytoplasm; however, a stain in nucleolus was also detected. Binding to cytoplasmic structures might occur due to high affinity of the compound to proteins revealed by spectroscopical methods.DiscussionActivation of DNA repair enzymes after binding to DNA appears to be the basis for the antimutagenic effects of AV-153-Na.

scholarly journals Confocal fluorescence microscopy: a powerful tool in the study of Chagas' disease

2000 ◽  
Vol 33 (1) ◽  
pp. 79-82 ◽  
Author(s):  
Renato Arruda Mortara ◽  
Solange da Silva ◽  
Noemi Nosomi Taniwaki
Keyword(s):  
Monoclonal Antibodies ◽  
Fluorescence Microscopy ◽  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Diagnostic Tool ◽  
Cell Biology ◽  
Confocal Fluorescence Microscopy ◽  
Confocal Fluorescence ◽  
Confocal Scanning ◽  
Powerful Diagnostic Tool

Confocal scanning fluorescence microscopy has become widely used in cell biology and pathology. In conjunction with monoclonal antibodies it may turn out to be a powerful diagnostic tool that also enables detailed studies of tissue forms of Trypanosoma cruzi.

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