Hyper-spectral imaging in scanning-confocal-fluorescence microscopy using a novel broadband diffractive optic

2014 ◽  
Vol 324 ◽  
pp. 73-80 ◽  
Author(s):  
Peng Wang ◽  
Carl G. Ebeling ◽  
Jordan Gerton ◽  
Rajesh Menon
Keyword(s):  
Fluorescence Microscopy ◽  
Spectral Imaging ◽  
Confocal Fluorescence Microscopy ◽  
Diffractive Optic ◽  
Hyper Spectral ◽  
Confocal Fluorescence ◽  
Hyper Spectral Imaging

Combining Total Internal Reflection Sum Frequency Spectroscopy Spectral Imaging and Confocal Fluorescence Microscopy

Langmuir ◽  
2015 ◽  
Vol 31 (3) ◽  
pp. 987-994 ◽  
Author(s):  
Edward S. Allgeyer ◽  
Sarah M. Sterling ◽  
Mudalige S. Gunewardene ◽  
Samuel T. Hess ◽  
David J. Neivandt ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Total Internal Reflection ◽  
Spectral Imaging ◽  
Internal Reflection ◽  
Confocal Fluorescence Microscopy ◽  
Sum Frequency ◽  
Sum Frequency Spectroscopy ◽  
Confocal Fluorescence

scholarly journals Analysis of Polyhydroxyalkanoates Granules in Haloferax mediterranei by Double-Fluorescence Staining with Nile Red and SYBR Green by Confocal Fluorescence Microscopy

Polymers ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1582
Author(s):  
Verónica Cánovas ◽  
Salvador Garcia-Chumillas ◽  
Fuensanta Monzó ◽  
Lorena Simó-Cabrera ◽  
Carmen Fernández-Ayuso ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Culture Media ◽  
Nile Red ◽  
Sybr Green ◽  
Confocal Fluorescence Microscopy ◽  
Fluorescence Staining ◽  
Haloferax Mediterranei ◽  
Optimized Protocol ◽  
Confocal Fluorescence ◽  
Pha Production

Haloferaxmediterranei is a haloarchaeon of high interest in biotechnology because it produces and mobilizes intracellular polyhydroxyalkanoate (PHA) granules during growth under stress conditions (limitation of phosphorous in the culture media), among other interesting metabolites (enzymes, carotenoids, etc.). The capability of PHA production by microbes can be monitored with the use of staining-based methods. However, the staining of haloarchaea cells is a challenging task; firstly, due to the high ionic strength of the medium, which is inappropriate for most of dyes, and secondly, due to the low permeability of the haloarchaea S-layer to macromolecules. In this work, Haloferax mediterranei is used as a halophilic archaeon model to describe an optimized protocol for the visualization and analysis of intracellular PHA granules in living cells. The method is based on double-fluorescence staining using Nile red and SYBR Green by confocal fluorescence microscopy. Thanks to this method, the capability of PHA production by new haloarchaea isolates could be easily monitored.

scholarly journals Detection of Waxed Chestnuts using Visible and Near-Infrared Hyper-spectral Imaging

2016 ◽  
Vol 22 (2) ◽  
pp. 267-277
Author(s):  
Baicheng Li ◽  
Baolu Hou ◽  
Yao Zhou ◽  
Mantong Zhao ◽  
Dawei Zhang ◽  
...  
Keyword(s):  
Near Infrared ◽  
Spectral Imaging ◽  
Hyper Spectral ◽  
Hyper Spectral Imaging

Analysis of background irradiation in thermal IR hyper-spectral imaging systems

2010 ◽  
Author(s):  
Weiming Xu ◽  
Liyin Yuan ◽  
Ying Lin ◽  
Zhiping He ◽  
Rong Shu ◽  
...  
Keyword(s):  
Spectral Imaging ◽  
Imaging Systems ◽  
Hyper Spectral ◽  
Hyper Spectral Imaging
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