Second harmonic generation polarization microscopy with tightly focused linearly and radially polarized beams

2007 ◽  
Vol 275 (2) ◽  
pp. 453-457 ◽  
Author(s):  
E.Y.S. Yew ◽  
C.J.R. Sheppard
Keyword(s):  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Second Harmonic ◽  
Polarization Microscopy ◽  
Polarized Beams ◽  
Radially Polarized

scholarly journals Second harmonic generation polarization microscopy as a tool for protein structure analysis

2018 ◽  
Author(s):  
Junichi Kaneshiro ◽  
Yasushi Okada ◽  
Tomohiro Shima ◽  
Mika Tsujii ◽  
Katsumi Imada ◽  
...  
Keyword(s):  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Conformational Changes ◽  
Second Harmonic ◽  
Coherent Scattering ◽  
Polarization Measurement ◽  
Molecular Structures ◽  
Polarization Microscopy ◽  
Protein Structure Analysis ◽  
Macromolecular Protein

AbstractSecond-harmonic generation (SHG) is a nonlinear coherent scattering process that is sensitive to molecular structures in illuminated materials. We report SHG polarization measurement for the detection of protein conformational changes in solutions of macromolecular protein assemblies such as microtubules and protein crystals. The results illustrate the potential of this method for protein structural analysis in physiological solutions at room temperature without labelling.

scholarly journals Second harmonic generation polarization microscopy as a tool for protein structure analysis

2019 ◽  
Vol 16 (0) ◽  
pp. 147-157 ◽  
Author(s):  
Junichi Kaneshiro ◽  
Yasushi Okada ◽  
Tomohiro Shima ◽  
Mika Tsujii ◽  
Katsumi Imada ◽  
...  
Keyword(s):  
Protein Structure ◽  
Second Harmonic Generation ◽  
Structure Analysis ◽  
Harmonic Generation ◽  
Second Harmonic ◽  
Polarization Microscopy ◽  
Protein Structure Analysis
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