Histochemical observation of bony reversal lines in bisphosphonate-related osteonecrosis of the jaw

Soung Min Kim; Mi Young Eo; Yeon Sook Kim; Suk Keun Lee

doi:10.1016/j.oooo.2016.09.225

Histochemical observation of bony reversal lines in bisphosphonate-related osteonecrosis of the jaw

Oral Surgery Oral Medicine Oral Pathology and Oral Radiology ◽

10.1016/j.oooo.2016.09.225 ◽

2017 ◽

Vol 123 (2) ◽

pp. 220-228 ◽

Cited By ~ 5

Author(s):

Soung Min Kim ◽

Mi Young Eo ◽

Yeon Sook Kim ◽

Suk Keun Lee

Keyword(s):

Osteonecrosis Of The Jaw ◽

Histochemical Observation

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A microorganism not to be overlooked in studies focusing on osteonecrosis of the jaws: Comment on “Kim SM, et al. Histochemical observation of bony reversal lines in bisphosphonate-related osteonecrosis of the jaw”

Oral Surgery Oral Medicine Oral Pathology and Oral Radiology ◽

10.1016/j.oooo.2016.12.015 ◽

2017 ◽

Vol 123 (6) ◽

pp. 738-739 ◽

Cited By ~ 1

Author(s):

Aydin Gülses ◽

Mustafa Ayna ◽

Yahya Açil

Keyword(s):

Osteonecrosis Of The Jaw ◽

Osteonecrosis Of The Jaws ◽

Histochemical Observation

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Enzyme histochemical application and backscattered electron imaging to visualize the distribution of lymphatic capillaries

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100161965 ◽

1990 ◽

Vol 48 (3) ◽

pp. 880-881

Author(s):

Seiji Kato

Keyword(s):

Staining Method ◽

Double Staining ◽

Reaction Products ◽

Blood Capillaries ◽

Backscattered Electron Imaging ◽

Histochemical Observation ◽

Backscattered Electron ◽

Double Staining Method ◽

Electron Imaging ◽

Cryostat Sections

Previously, the author repeatedly confirmed the higher 5’-nucleotidase (5’-Nase) and lower alkaline phoaphatase (ALPase) activities in the wall of lymphatic capillaries reacted with the lead-based method relative to those of blood capillaries. The ALPase, on the other hand, is markedly higher in blood capillaries than in lymphatics. On the basis of these enzyme characteristics, the author has developed a 5’-Nase— ALPase double staining method to differentiate small lymphatics from blood capillaries at the level of the light microcsopy. Furthermore, we applied it to histochemical observation of the lead-containing reaction products of 5’-Nase in lymphatics on the same or adjacent cryostat sections using backscattered electron imaging (BEI) in scanning electron microscope (SEM). This paper presents a new applicability of 5’-Nase histochemistry by BEI-SEM to demonstrate the distribution of lymphatic capillaries in tissue blocks.

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