Silk proteins stimulate osteoblast differentiation by suppressing the Notch signaling pathway in mesenchymal stem cells

2013 ◽  
Vol 33 (2) ◽  
pp. 162-170 ◽  
Author(s):  
So-Ra Jung ◽  
No-Joon Song ◽  
Dong Kwon Yang ◽  
Yong-Jun Cho ◽  
Byung-Joon Kim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Notch Signaling ◽  
Signaling Pathway ◽  
Osteoblast Differentiation ◽  
Notch Signaling Pathway ◽  
Silk Proteins ◽  
Stimulate Osteoblast Differentiation

scholarly journals Cordycepin inhibits chondrocyte hypertrophy of mesenchymal stem cells through PI3K/Bapx1 and Notch signaling pathway

BMB Reports ◽  
2016 ◽  
Vol 49 (10) ◽  
pp. 548-553 ◽  
Author(s):  
Zhen Cao ◽  
Ce Dou ◽  
Jianmei Li ◽  
Xiangyu Tang ◽  
Junyu Xiang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Notch Signaling ◽  
Signaling Pathway ◽  
Notch Signaling Pathway ◽  
Chondrocyte Hypertrophy

scholarly journals Effect of NTN and Lmx1α on the Notch Signaling Pathway during the Differentiation of Human Bone Marrow Mesenchymal Stem Cells into Dopaminergic Neuron-Like Cells

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Jinhua Zhang ◽  
Bo Yang ◽  
Lilin Luo ◽  
Linhui Li ◽  
Xuantao Yang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Notch Signaling ◽  
Signaling Pathway ◽  
Dopaminergic Neuron ◽  
Notch Signaling Pathway ◽  
Control Group ◽  
Marrow Mesenchymal Stem Cells

Human bone marrow mesenchymal stem cells (h-BMSCs) have the potential to differentiate into dopaminergic neuron-like cells to treat Parkinson’s disease. The Notch signaling pathway has been implicated in the regulation of cell fate decisions such as differentiation of BMSCs. This study investigated changes in the expression of Notch-related genes in the differentiation of BMSCs in vitro into dopaminergic (DA) neuron-like cells. BMSCs transfected with empty lentiviral vectors served as the control group and those transfected with NTN and Lmx1α recombinant lentiviral vectors served as the experimental group. After induction and culture of NTN and Lmx1α-transfected h-BMSCs for 21 days, the cells exhibited features of dopaminergic neuron-like cells, which were observed by transmission and scanning electron microscopy and verified by immunofluorescence of tyrosine hydroxylase (TH) and dopamine transporter (DAT). These induced cells could secrete dopamine and had basic action potentials. Expression of the neural stem cell (NSC) markers, including octamer-binding protein (Oct4), paired box gene 6 (Pax6), and sex determining region Y-box 1 (SOX1), increased on day 14 of induction and decreased on day 21 of induction during differentiation. The human Notch signaling pathway PCR array showed a differential expression of Notch-related genes during the differentiation of h-BMSCs into DA neuron-like cells in vitro relative to that in the control group. In conclusion, h-BMSCs overexpressing NTN and Lmx1α can successfully be induced to differentiate into dopaminergic neuron-like cells with a neuronal phenotype exhibiting fundamental biological functions in vitro, and NTN and Lmx1α may affect the expression of Notch-related genes during differentiation.

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