Induction of alkaline phosphatase activity by l-ascorbic acid in human osteoblastic cells: a potential role for CK2 and Ikaros

Nutrition ◽  
2007 ◽  
Vol 23 (10) ◽  
pp. 745-753 ◽  
Author(s):  
Eunwha Son ◽  
Hang Do ◽  
Hae-Mi Joo ◽  
Suhkneung Pyo
Keyword(s):  
Alkaline Phosphatase ◽  
Ascorbic Acid ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Potential Role ◽  
Osteoblastic Cells ◽  
Human Osteoblastic Cells

scholarly journals Cytocompatibility evaluation of hydroxyapatite/collagen composites doped with Zn+2

2007 ◽  
Vol 12 (2) ◽  
pp. 307-312 ◽  
Author(s):  
Maria Helena Santos ◽  
Ana Paula M. Shaimberg ◽  
Patricia Valerio ◽  
Alfredo M. Goes ◽  
Maria de Fátima Leite ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Orthophosphoric Acid ◽  
Type I Collagen ◽  
Enzymatic Digestion ◽  
Osteoblastic Cells ◽  
Type I ◽  
Post Test ◽  
Synthetic Hydroxyapatite

The cytocompatibility of synthetic hydroxyapatite/collagen composites alone or doped with Zn+2 was tested by using primary culture of osteoblasts. The hydroxyapatite (HAP) was synthesized having calcium hydroxide and orthophosphoric acid as precursors. A new HAP composite was developed adding 1.05 w% of Zn(NO3)2.6H2O forming HAPZn. The pure type I collagen (COL) was obtained from bovine pericardium by enzymatic digestion method. The HAP/COL and HAPZn/COL composites were developed and characterized by SEM/EDS. The cell viability and alkaline phosphatase activity in the presence of composites were evaluated by MTT assay and NBT-BCIP assay, respectively, and compared to osteoblastic cells of the control. Three individual experiments were accomplished in triplicates and submitted to the variance analysis and Bonferroni’s post-test with statistically significant at p<0.05. The HAPZn/COL composite did not stimulate the proliferation and increasing of alkaline phosphatase activity of the osteoblastic cells. The tested composites did not alter the cellular viability neither caused alterations in the cellular morphology in 72 h showing adequate properties for biological applications.

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