Methylenetetrahydrofolate reductase polymorphism determines the plasma homocysteine-lowering effect of large-dose folic acid supplementation in patients with cardiovascular disease

Nutrition ◽  
2004 ◽  
Vol 20 (11-12) ◽  
pp. 974-978 ◽  
Author(s):  
Chin-San Liu ◽  
Hui-Chin Chiang ◽  
Haw-Wen Chen
Keyword(s):  
Cardiovascular Disease ◽  
Folic Acid ◽  
Large Dose ◽  
Methylenetetrahydrofolate Reductase ◽  
Folic Acid Supplementation ◽  
Plasma Homocysteine ◽  
Acid Supplementation ◽  
Lowering Effect

Folic acid attenuates homocysteine and enhances antioxidative capacity in atherosclerotic rats

2017 ◽  
Vol 42 (10) ◽  
pp. 1015-1022 ◽  
Author(s):  
Shanshan Cui ◽  
Wen Li ◽  
Xin Lv ◽  
Pengyan Wang ◽  
Guowei Huang ◽  
...  
Keyword(s):  
Folic Acid ◽  
Antioxidant Capacity ◽  
Wistar Rats ◽  
Folic Acid Supplementation ◽  
Plasma Homocysteine ◽  
Serum Folate ◽  
Antioxidative Capacity ◽  
High Fat ◽  
Acid Supplementation ◽  
Atherosclerotic Lesions

Atherosclerosis is a chronic disease that can seriously endanger human life. Folic acid supplementation modulates several disorders, including atherosclerosis, via its antiapoptotic and antioxidative properties. This study investigated whether folic acid alleviates atherogenesis by restoring homocysteine levels and antioxidative capacity in atherosclerosis Wistar rats. To this end, 28 Wistar rats were randomly divided into 4 groups (7 rats/group) as follows: (i) wild-type group, fed only the AIN-93 semi-purified rodent diet (folic acid: 2.1 mg/kg); (ii) high-fat + folic acid-deficient group (HF+DEF) (folic acid: 0.2 mg/kg); (iii) high-fat + normal folic acid group (folic acid: 2.1 mg/kg); and (iv) high-fat + folic acid-supplemented group (folic acid: 4.2 mg/kg). After 12 weeks, histopathological changes in the atherosclerotic lesions of the aortic arch were determined. In addition, serum folate levels, plasma homocysteine levels, plasma S-adenosyl-homocysteine levels, antioxidant status, oxidant status, and lipid profiles were evaluated. The results show aggravated atherosclerotic lesions in the HF+DEF group. Folic acid supplementation increased concentrations of serum folate. Further, folic acid supplementation increased high-density lipoprotein-cholesterol, decreased plasma homocysteine levels, and improved antioxidant capacity in atherogenic rats. These findings are consistent with the hypothesis that folic acid alleviates atherogenesis by reducing plasma homocysteine levels and improving antioxidant capacity in rats fed a high-fat diet.

Oxidative stress and platelet activation in subjects with moderate hyperhomocysteinaemia due to MTHFR 677 C→T polymorphism

2012 ◽  
Vol 108 (09) ◽  
pp. 533-542 ◽  
Author(s):  
Alfredo Dragani ◽  
Angela Falco ◽  
Francesca Santilli ◽  
Stefania Basili ◽  
Giancarlo Rolandi ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Folic Acid ◽  
Platelet Activation ◽  
Methylenetetrahydrofolate Reductase ◽  
Folic Acid Supplementation ◽  
Control Group ◽  
Loading Test ◽  
Study Objective ◽  
Acid Supplementation

SummaryThe methylenetetrahydrofolate reductase (MTHFR) 677 C→T polymorphism may be associated with elevated total homocysteine (tHcy) levels, an independent risk factor for cardiovascular disease. It was the study objective to evaluate in vivo lipid peroxidation and platelet activation in carriers of the MTHFR 677 C→T polymorphism and in non-carriers, in relation to tHcy and folate levels. A cross-sectional comparison of urinary 8-iso-prostaglandin (PG)F2α and 11-dehydro-thromboxane (TX)B2 (markers of in vivo lipid peroxidation and platelet activation, respectively) was performed in 100 carriers and 100 non-carriers of the polymorphism. A methionine-loading test and folic acid supplementation were performed to investigate the causal relationship of the observed associations. Urinary 8-iso-PGF2α and 11-dehydro-TXB2 were higher in carriers with hyperhomocysteinaemia than in those without hyperhomocysteinaemia (p<0.0001). Hyperhomocysteinaemic carriers had lower folate levels (p=0.0006), higher urinary 8-iso-PGF2α (p<0.0001) and 11-dehydro-TXB2 (p<0.0001) than hyperhomocysteinaemic non-carriers. On multiple regression analysis, high tHcy (p<0.0001), low folate (p<0.04) and MTHFR 677 C→T polymorphism (p<0.001) independently predicted high rates of 8-iso-PGF2α excretion. Methionine loading increased plasma tHcy (p=0.002), and both urinary prostanoid metabolites (p=0.002). Folic acid supplementation was associated with decreased urinary 8-iso-PGF2α and 11-dehydro-TXB2 excretion (p<0.0003) in the hyperhomocysteinaemic group, but not in the control group, with substantial inter-individual variability related to baseline tHcy level and the extent of its reduction. In conclusion, hyperhomocysteinaemia due to the MTHFR 677 C→T polymorphism is associated with enhanced in vivo lipid peroxidation and platelet activation that are reversible, at least in part, following folic acid supplementation. An integrated biomarker approach may help identifying appropriate candidates for effective folate supplementation.

Folic acid supplementation reduces plasma homocysteine in postmenopausal women

2016 ◽  
Vol 36 (4) ◽  
pp. 492-495 ◽  
Author(s):  
Fariba Almassinokiani ◽  
Maryam Kashanian ◽  
Peyman Akbari ◽  
Elaheh Mossayebi ◽  
Elena Sadeghian
Keyword(s):  
Folic Acid ◽  
Postmenopausal Women ◽  
Folic Acid Supplementation ◽  
Plasma Homocysteine ◽  
Acid Supplementation
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