Characterizing the effects of in utero valproic acid exposure on NF-κB signaling in CD-1 mouse embryos during neural tube closure

2021 ◽  
Vol 83 ◽  
pp. 106941
Author(s):  
Sidra Shafique ◽  
Louise M. Winn
Keyword(s):  
Valproic Acid ◽  
Neural Tube ◽  
In Utero ◽  
Mouse Embryos ◽  
Acid Exposure ◽  
Neural Tube Closure ◽  
Tube Closure

scholarly journals Valproic acid disrupts the biomechanics of late spinal neural tube closure in mouse embryos

2018 ◽  
Vol 149 ◽  
pp. 20-26 ◽  
Author(s):  
Amy Hughes ◽  
Nicholas D.E. Greene ◽  
Andrew J. Copp ◽  
Gabriel L. Galea
Keyword(s):  
Valproic Acid ◽  
Neural Tube ◽  
Mouse Embryos ◽  
Neural Tube Closure ◽  
Tube Closure

scholarly journals Biomechanical coupling facilitates spinal neural tube closure in mouse embryos

2017 ◽  
pp. 201700934 ◽  
Author(s):  
Gabriel L. Galea ◽  
Young-June Cho ◽  
Gauden Galea ◽  
Matteo A. Molè ◽  
Ana Rolo ◽  
...  
Keyword(s):  
Neural Tube ◽  
Mouse Embryos ◽  
Neural Tube Closure ◽  
Tube Closure

scholarly journals In utero gene transfer system for embryos before neural tube closure reveals a role for Hmga2 in the onset of neurogenesis

2020 ◽  
Author(s):  
Naohiro Kuwayama ◽  
Yusuke Kishi ◽  
Yurie Maeda ◽  
Yurie Nishiumi ◽  
Yutaka Suzuki ◽  
...  
Keyword(s):  
Gene Expression ◽  
Neural Tube ◽  
Neural Development ◽  
Viral Vectors ◽  
Early Stage ◽  
Cell Types ◽  
In Utero ◽  
Polycomb Group ◽  
Neural Tube Closure ◽  
Tube Closure

AbstractDuring the early stage of mammalian neural development, neuroepithelial cells (NECs) proliferate and increase their pool size before switching their fate to become neurogenic radial glial progenitors (RGPs). The timing of this expansion-to-neurogenic phase transition is strictly regulated so as to determine the proper number of progenitors and differentiated cell types that constitute the brain. The molecular mechanism underlying this switch has remained poorly understood, however, in part because of the difficulty associated with manipulation of gene expression in NECs before their transition to RGPs, which occurs before neural tube closure. We have now developed a simple and efficient method to manipulate gene expression in mouse neocortical NECs in the expansion phase by in utero injection of viral vectors at embryonic day 7.0 to 8.0. With the use of this method, we found that knockdown of the chromatin-associated protein Hmga2 in NECs inhibited the onset of the neurogenic phase in the neocortex. This effect of Hmga2 knockdown was accompanied by inhibition of the expression of a set of genes targeted by Polycomb group proteins, which repress neurogenic genes in cortical progenitors. Our study thus establishes a novel method for manipulation of gene expression in the early stage of mouse brain development as well as uncovers a key molecular player in the generation of neurogenic progenitors in the developing mouse neocortex.

Curvature of the caudal region is responsible for failure of neural tube closure in the curly tail (ct) mouse embryo

Development ◽  
1991 ◽  
Vol 113 (2) ◽  
pp. 671-678 ◽  
Author(s):  
F.A. Brook ◽  
A.S. Shum ◽  
H.W. Van Straaten ◽  
A.J. Copp
Keyword(s):  
Neural Tube ◽  
Mouse Embryo ◽  
Mouse Embryos ◽  
The Body ◽  
Neural Tube Closure ◽  
Posterior Neuropore ◽  
Curly Tail ◽  
Delayed Closure ◽  
Tube Closure

Delayed closure of the posterior neuropore (PNP) occurs to a variable extent in homozygous mutant curly tail (ct) mouse embryos, and results in the development of spinal neural tube defects (NTD) in 60% of embryos. Previous studies have suggested that curvature of the body axis may delay neural tube closure in the cranial region of the mouse embryo. In order to investigate the relationship between curvature and delayed PNP closure, we measured the extent of ventral curvature of the neuropore region in ct/ct embryos with normal or delayed PNP closure. The results show significantly greater curvature in ct/ct embryos with delayed PNP closure in vivo than in their normal littermates. Reopening of the posterior neuropore in non-mutant mouse embryos, to delay neuropore closure experimentally, did not increase ventral curvature, suggesting that increased curvature in ct/ct embryos is not likely to be a secondary effect of delayed PNP closure. Experimental prevention of ventral curvature in ct/ct embryos, brought about by implantation of an eyelash tip longitudinally into the hindgut lumen, ameliorated the delay in PNP closure. We propose, therefore, that increased ventral curvature of the neuropore region of ct/ct embryos imposes a mechanical stress, which opposes neurulation and thus delays closure of the PNP. Increased ventral curvature may arise as a result of a cell proliferation imbalance, which we demonstrated previously in affected ct/ct embryos.

Spatio-temporal curvature pattern of the caudal body axis for non-mutant and curly tail mouse embryos during the period of caudal neural tube closure

1997 ◽  
Vol 195 (3) ◽  
pp. 259-266 ◽  
Author(s):  
M. C. E. Peeters ◽  
Johan W. M. Hekking ◽  
Tryfon Vainas ◽  
J. Drukker ◽  
Henny W. M. van Straaten
Keyword(s):  
Neural Tube ◽  
Mouse Embryos ◽  
Body Axis ◽  
Neural Tube Closure ◽  
Curly Tail ◽  
Spatio Temporal ◽  
Tube Closure

scholarly journals Proper expression of the Gcn5 histone acetyltransferase is required for neural tube closure in mouse embryos

2008 ◽  
Vol 237 (4) ◽  
pp. 928-940 ◽  
Author(s):  
Wenchu Lin ◽  
Zhijing Zhang ◽  
Geraldine Srajer ◽  
Yi Chun Chen ◽  
Maosheng Huang ◽  
...  
Keyword(s):  
Neural Tube ◽  
Histone Acetyltransferase ◽  
Mouse Embryos ◽  
Neural Tube Closure ◽  
Tube Closure

scholarly journals Excess methionine suppresses the methylation cycle and inhibits neural tube closure in mouse embryos

FEBS Letters ◽  
2006 ◽  
Vol 580 (11) ◽  
pp. 2803-2807 ◽  
Author(s):  
Louisa P.E. Dunlevy ◽  
Katie A. Burren ◽  
Lyn S. Chitty ◽  
Andrew J. Copp ◽  
Nicholas D.E. Greene
Keyword(s):  
Neural Tube ◽  
Mouse Embryos ◽  
Neural Tube Closure ◽  
Tube Closure

scholarly journals Methylmercury induced toxicogenomic response in C57 and SWV mouse embryos undergoing neural tube closure

2010 ◽  
Vol 30 (2) ◽  
pp. 284-291 ◽  
Author(s):  
Joshua F. Robinson ◽  
William C. Griffith ◽  
Xiaozhong Yu ◽  
Sungwoo Hong ◽  
Euvin Kim ◽  
...  
Keyword(s):  
Neural Tube ◽  
Mouse Embryos ◽  
Neural Tube Closure ◽  
Tube Closure
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