scholarly journals Insights into nitric oxide-mediated water balance, antioxidant defence and mineral homeostasis in rice (Oryza sativa L.) under chilling stress

Nitric Oxide ◽  
2020 ◽  
Vol 100-101 ◽  
pp. 7-16 ◽  
Author(s):  
Abdullah Al Mamun Sohag ◽  
Md Tahjib-Ul-Arif ◽  
Sonya Afrin ◽  
Md Kawsar Khan ◽  
Md Abdul Hannan ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Oryza Sativa ◽  
Water Balance ◽  
Oryza Sativa L ◽  
Chilling Stress ◽  
Antioxidant Defence ◽  
Mineral Homeostasis

scholarly journals Insights into Nitric Oxide-Mediated Water Balance, Antioxidant Defence and Mineral Homeostasis in Rice (Oryza sativa L.) under Chilling Stress

2020 ◽  
Author(s):  
Abdullah Al Mamun Sohag ◽  
Md Tahjib-Ul-Arif ◽  
Sonya Afrin ◽  
Md Kawsar Khan ◽  
Md. Abdul Hannan ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Oryza Sativa ◽  
Molecular Mechanisms ◽  
Oryza Sativa L ◽  
Chilling Stress ◽  
Soluble Sugar ◽  
Chilling Injury ◽  
Chilling Tolerance ◽  
No Donor ◽  
Mineral Homeostasis

Being a chilling-sensitive staple crop, rice (Oryza sativa L.) is vulnerable to climate change. The competence of rice to withstand chilling stress should, therefore, be enhanced through technological tools. The present study employed chemical intervention like application of sodium nitroprusside (SNP) as nitric oxide (NO) donor and elucidated the underlying molecular mechanisms of NO-mediated chilling tolerance in rice. At germination stage, germination indicators were interrupted by chilling stress (5.0 ± 1.0°C for 8 h day‒1), while pretreatment with 100 μM SNP markedly improved the indicators. At seedling stage (14-day-old), chilling stress caused stunted growth with visible toxicity along with alteration of biochemical markers, for example, increase in oxidative stress markers (superoxide, hydrogen peroxide, and malondialdehyde) and osmolytes (total soluble sugar; proline and soluble protein content, SPC), and decrease in chlorophyll (Chl), relative water content (RWC), and antioxidants. However, NO application attenuated toxicity symptoms with improving growth performance which might be attributed to enhanced activities of antioxidants, mineral contents, Chl, RWC and SPC. Furthermore, principal component analysis indicated that water imbalance and increased oxidative damage were the main contributors to chilling injury, whereas NO-mediated mineral homeostasis and antioxidant defense were the critical determinants for chilling tolerance in rice. Collectively, our findings revealed that NO protects against chilling stress through valorizing cellular defense mechanisms, suggesting that exogenous application of NO could be a potential tool to evolve cold tolerance as well as climate resilience in rice.

scholarly journals Reactive Oxygen Species (ROS) Metabolism and Nitric Oxide (NO) Content in Roots and Shoots of Rice (Oryza sativa L.) Plants under Arsenic-Induced Stress

Agronomy ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 1014 ◽  
Author(s):  
Ernestina Solórzano ◽  
Francisco J. Corpas ◽  
Salvador González-Gordo ◽  
José M. Palma
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Reactive Oxygen Species ◽  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Antioxidative Systems ◽  
Ros Metabolism ◽  
As Stress

Arsenic (As) is a highly toxic metalloid for all forms of life including plants. Rice is the main food source for different countries worldwide, although it can take up high amounts of As in comparison with other crops, showing toxic profiles such as decreases in plant growth and yield. The induction of oxidative stress is the main process underlying arsenic toxicity in plants, including rice, due to an alteration of the reactive oxygen species (ROS) metabolism. The aim of this work was to gain better knowledge on how the ROS metabolism and its interaction with nitric oxide (NO) operate under As stress conditions in rice plants. Thus, physiological and ROS-related biochemical parameters in roots and shoots from rice (Oryza sativa L.) were studied under 50 μM arsenate (AsV) stress, and the involvement of the main antioxidative systems and NO in the response of plants to those conditions was investigated. A decrease of 51% in root length and 27% in plant biomass was observed with 50 μM AsV treatment, as compared to control plants. The results of the activity of superoxide dismutase (SOD) isozymes, catalase, peroxidase (POD: total and isoenzymatic), and the enzymes of the ascorbate–glutathione cycle, besides the ascorbate and glutathione contents, showed that As accumulation provoked an overall significant increase of most of them, but with different profiles depending on the plant organ, either root or shoot. Among the seven identified POD isozymes, the induction of the POD-3 in shoots under As stress could help to maintain the hydrogen peroxide (H2O2) redox homeostasis and compensate the loss of the ascorbate peroxidase (APX) activity in both roots and shoots. Lipid peroxidation was slightly increased in roots and shoots from As-treated plants. The H2O2 and NO contents were enhanced in roots and shoots against arsenic stress. In spite of the increase of most antioxidative systems, a mild oxidative stress situation appears to be consolidated overall, since the growth parameters and those from the oxidative damage could not be totally counteracted. In these conditions, the higher levels of H2O2 and NO suggest that signaling events are simultaneously occurring in the whole plant.

S-nitrosocysteine-responsive genes modulate diverse regulatory pathways in Oryza sativa: a transcriptome profiling study

2018 ◽  
Vol 45 (6) ◽  
pp. 630 ◽  
Author(s):  
Bong-Gyu Mun ◽  
Sang-Uk Lee ◽  
Adil Hussain ◽  
Hyun-Ho Kim ◽  
Nkulu Kabange Rolly ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Oryza Sativa ◽  
Transcriptional Control ◽  
Nitrosative Stress ◽  
Genetic Model ◽  
Oryza Sativa L ◽  
Expression Patterns ◽  
Nitric Oxide Donor ◽  
Transcriptional Networks ◽  
Regulatory Pathways

Rice (Oryza sativa L.) is a major food crop and also a well-established genetic model. Nitric oxide (NO) and its derivatives are important signalling molecules that actively participate in various signalling pathways in response to different stresses. In this study, we performed RNA-seq mediated transcriptomic analysis of rice after treatment with the nitric oxide donor, S-nitroso-L-cysteine (CySNO), generating an average of 37.5 and 41.5 million reads from control and treated leaf samples respectively. More than 95% of the reads were successfully mapped to the O. sativa reference genome yielding a total of 33 539 differentially expressed genes (DEGs, P < 0.05). Further analyses identified 825 genes with at least 2-fold change in the expression following treatment with CySNO (P < 0.01). The DEGs identified were involved in diverse molecular functions such as catalytic activity, binding, transport, and receptor activity and were mostly located in the membrane, organelles such as nucleus, Golgi apparatus and mitochondria. DEGs also contained several genes that regulate responses to abiotic stresses such as drought, heat, cold and salt stress and biotic stresses. We also found significantly similar expression patterns of CySNO-responsive DEGs of rice with the CySNO-responsive DEGs of Arabidopsis in a previous study. Expression patterns of genes involved in key biological functions were verified using quantitative real time (qRT)-PCR. The findings of this study suggest that NO regulates the transcriptional control of genes involved in a wide variety of physiological functions in rice, and that NO-mediated transcriptional networks are highly conserved across the plant kingdom. This study provides useful information regarding the transcriptional response of plants to nitrosative stress.

Silicon mediates arsenic tolerance in rice (Oryza sativa L.) through lowering of arsenic uptake and improved antioxidant defence system

2013 ◽  
Vol 52 ◽  
pp. 96-103 ◽  
Author(s):  
Preeti Tripathi ◽  
Rudra Deo Tripathi ◽  
Rana Pratap Singh ◽  
Sanjay Dwivedi ◽  
Dheeraj Goutam ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Antioxidant Defence ◽  
Arsenic Uptake ◽  
Arsenic Tolerance ◽  
Defence System ◽  
Antioxidant Defence System

scholarly journals Effect of Nitric Oxide on Alleviating Cadmium Toxicity in Rice (Oryza sativa L.)

2013 ◽  
Vol 12 (9) ◽  
pp. 1540-1550 ◽  
Author(s):  
Xiu-feng ZHAO ◽  
Lin CHEN ◽  
Muhammad I A Rehmani ◽  
Qiang-sheng WANG ◽  
Shao-hua WANG ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Cadmium Toxicity

Cadmium stress-induced oxidative stress and role of nitric oxide in rice (Oryza sativa L.)

2011 ◽  
Vol 33 (5) ◽  
pp. 1737-1747 ◽  
Author(s):  
Piyalee Panda ◽  
Shwetosmita Nath ◽  
Th. Thorny Chanu ◽  
Gauri Dutta Sharma ◽  
Sanjib Kumar Panda
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Cadmium Stress

Cold sensitivity in rice (Oryza sativa L.) is strongly correlated with a naturally occurring I99V mutation in the multifunctional glutathione transferase isoenzyme GSTZ2

2011 ◽  
Vol 435 (2) ◽  
pp. 373-380 ◽  
Author(s):  
Sang-Ic Kim ◽  
Virgilio C. Andaya ◽  
Thomas H. Tai
Keyword(s):  
Oryza Sativa ◽  
Glutathione Transferase ◽  
Oryza Sativa L ◽  
Chilling Stress ◽  
Dichloroacetic Acid ◽  
Cold Sensitivity ◽  
Nucleotide Polymorphisms ◽  
Naturally Occurring ◽  
Tyrosine Metabolism ◽  
Trait Locus

GSTZs [Zeta class GSTs (glutathione transferases)] are multifunctional enzymes that belong to a highly conserved subfamily of soluble GSTs found in species ranging from fungi and plants to animals. GSTZs are known to function as MAAIs [MAA (maleylacetoacetate) isomerases], which play a role in tyrosine catabolism by catalysing the isomerization of MAA to FAA (fumarylacetoacetate). As tyrosine metabolism in plants differs from animals, the significance of GSTZ/MAAI is unclear. In rice (Oryza sativa L.), a major QTL (quantitative trait locus) for seedling cold tolerance has been fine mapped to a region containing the genes OsGSTZ1 and OsGSTZ2. Sequencing of tolerant (ssp. japonica cv. M-202) and sensitive (ssp. indica cv. IR50) cultivars revealed two SNPs (single nucleotide polymorphisms) in OsGSTZ2 that result in amino acid differences (I99V and N184I). Recombinant OsGSTZ2 containing the Val99 residue found in IR50 had significantly reduced activity on MAA and DCA (dichloroacetic acid), but the Ile184 residue had no effect. The distribution of the SNP (c.295A>G) among various rice accessions indicates a significant association with chilling sensitivity in rice seedlings. The results of the present study show that naturally occurring OsGSTZ2 isoforms differ in their enzymatic properties, which may contribute to the differential response to chilling stress generally exhibited by the two major rice subspecies.

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