Subarachnoid hemorrhage induces neuronal nitric oxide synthase phosphorylation at Ser1412 in the dentate gyrus of the rat brain

Nitric Oxide ◽  
2018 ◽  
Vol 81 ◽  
pp. 67-74 ◽  
Author(s):  
Kentaro Wada ◽  
Koji Osuka ◽  
Yasuo Watanabe ◽  
Nobuteru Usuda ◽  
Motoaki Fukasawa ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Subarachnoid Hemorrhage ◽  
Nitric Oxide Synthase ◽  
Dentate Gyrus ◽  
Rat Brain ◽  
Neuronal Nitric Oxide Synthase ◽  
Oxide Synthase

scholarly journals Perinatal Lead Exposure Alters the Expression of Neuronal Nitric Oxide Synthase in Rat Brain

2001 ◽  
Vol 20 (3) ◽  
pp. 113-120 ◽  
Author(s):  
C. S. Chetty ◽  
G. R. Reddy ◽  
K. S. Murthy ◽  
J. Johnson ◽  
K. Sajwan ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nervous System ◽  
Enzyme Activity ◽  
Nitric Oxide Synthase ◽  
Rat Brain ◽  
Cognitive Deficits ◽  
Neuronal Nitric Oxide Synthase ◽  
Pb Exposure ◽  
Oxide Synthase ◽  
Developing Nervous System

Environmental exposure to lead (Pb) is known to affect the developing nervous system causing cognitive deficits in children. The diffusible nitric oxide (NO) is a biological messenger known to be involved in brain development. We examined the developmental changes of neuronal nitric oxide synthase (nNOS) in cerebellum and hippocampus of developing rat brain by radiometric assay, Western blot analysis and immunohistochemistry. Pb-exposure (0.2% Pb acetate) was initiated on gestation day 6 through the drinking water of the dam and continued through birth and postnatal days (PNDs) 1 to 21. The pups were never exposed to Pb directly. Pb exposure was stopped on weaning of pups from mothers on PND 21. The changes in nNOS were measured in the offspring on PNDs 7, 14, 21, and 35. The nNOS activity was increased gradually from PNDs 7 to 35 in both cerebellum and hippocampus of control rats when the enzyme activity was determined in the presence of either 0.5 or 6 μM calcium (Ca2+) in the reaction mixture. However, Pb exposure decreased the nNOS activity significantly at PNDs 21 to 35 as compared to respective controls when the enzyme activity was determined in the presence of 6 μM Ca2+. The decrease of nNOS was even greater and evident at all PNDs tested when the enzyme activity was assayed in the presence of physiological concentration of Ca2+ (0.5 μM). These findings were further strengthened by the in vitro studies. The cerebellar nNOS activity was inhibited much more at low Ca2+ (0.5 μM) as compared to 6 μM Ca2+, with IC50 values of 35 and 50 nM Pb, respectively. The nNOS protein levels and immunoreactivity in the cerebellum and hippocampus of rats perinatally exposed to Pb were decreased as compared to controls at PNDs 21 and 35. These data suggest perinatal Pb exposure decreases the nNOS in the developing brain. The decrease of nNOS activity and protein may explain the Pb-mediated cognitive deficits because NO regulates long-term potentiation (LTP) and other neurophysiological events in the developing nervous system.

scholarly journals Interaction of Neuronal Nitric-oxide Synthase with α1-Syntrophin in Rat Brain

1999 ◽  
Vol 274 (17) ◽  
pp. 11736-11741 ◽  
Author(s):  
Akiko Hashida-Okumura ◽  
Nobuaki Okumura ◽  
Akihiro Iwamatsu ◽  
Ruud M. Buijs ◽  
Herms J. Romijn ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Rat Brain ◽  
Neuronal Nitric Oxide Synthase ◽  
Oxide Synthase

scholarly journals The Coexpression of Reelin and Neuronal Nitric Oxide Synthase in a Subpopulation of Dentate Gyrus Neurons Is Downregulated in Heterozygous Reeler Mice

2010 ◽  
Vol 2010 ◽  
pp. 1-10 ◽  
Author(s):  
Raquel Romay-Tallón ◽  
Iria G. Dopeso-Reyes ◽  
April L. Lussier ◽  
Lisa E. Kalynchuk ◽  
Hector J. Caruncho
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Dentate Gyrus ◽  
Matrix Protein ◽  
Molecular Layer ◽  
Neuronal Nitric Oxide Synthase ◽  
Stratum Radiatum ◽  
Extracellular Matrix Protein ◽  
Subgranular Zone ◽  
Oxide Synthase

Reelin is an extracellular matrix protein expressed in several interneuron subtypes in the hippocampus and dentate gyrus. Neuronal nitric oxide synthase (nNOS) is also expressed by interneurons in these areas. We investigated whether reelin and nNOS are co-localized in the same population of hippocampal interneurons, and whether this colocalization is altered in the heterozygous reeler mouse. We found colocalization of nNOS in reelin-positive cells in the CA1 stratum radiatum and lacunosum moleculare, the CA3 stratum radiatum, and the dentate gyrus subgranular zone, molecular layer, and hilus. In heterozygous reeler mice, the colocalization of nNOS in reelin-positive cells was significantly decreased only in the subgranular zone and molecular layer. The coexpression of reelin and nNOS in several hippocampal regions suggests that reelin and nNOS may work synergistically to promote glutamatergic function, and the loss of this coexpression in heterozygous reeler mice may underlie some of the behavioral deficits observed in these animals.

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