Nitric oxide improves the hemodynamic performance of the hypoxic goldfish (Carassius auratus) heart

Nitric Oxide ◽  
2014 ◽  
Vol 42 ◽  
pp. 24-31 ◽  
Author(s):  
S. Imbrogno ◽  
C. Capria ◽  
B. Tota ◽  
F.B. Jensen
Keyword(s):  
Nitric Oxide ◽  
Carassius Auratus ◽  
Goldfish Carassius Auratus ◽  
Hemodynamic Performance

scholarly journals The Hypoxia Tolerance of the Goldfish (Carassius auratus) Heart: The NOS/NO System and Beyond

Antioxidants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 555 ◽  
Author(s):  
Mariacristina Filice ◽  
Rosa Mazza ◽  
Serena Leo ◽  
Alfonsina Gattuso ◽  
Maria Carmela Cerra ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Carassius Auratus ◽  
Ex Vivo ◽  
Acute Hypoxia ◽  
Protein S ◽  
Elisa Test ◽  
Hypoxia Tolerance ◽  
No Production ◽  
Goldfish Carassius Auratus ◽  
Enhanced Expression

The extraordinary capacity of the goldfish (Carassius auratus) to increase its cardiac performance under acute hypoxia is crucial in ensuring adequate oxygen supply to tissues and organs. However, the underlying physiological mechanisms are not yet completely elucidated. By employing an ex vivo working heart preparation, we observed that the time-dependent enhancement of contractility, distinctive of the hypoxic goldfish heart, is abolished by the Nitric Oxide Synthase (NOS) antagonist L-NMMA, the Nitric Oxide (NO) scavenger PTIO, as well as by the PI3-kinase (PI3-K) and sarco/endoplasmic reticulum Ca2+-ATPase 2a (SERCA2a) pumps’ inhibition by Wortmannin and Thapsigargin, respectively. In goldfish hearts exposed to hypoxia, an ELISA test revealed no changes in cGMP levels, while Western Blotting analysis showed an enhanced expression of the phosphorylated protein kinase B (pAkt) and of the NADPH oxidase catalytic subunit Nox2 (gp91phox). A significant decrease of protein S-nitrosylation was observed by Biotin Switch assay in hypoxic hearts. Results suggest a role for a PI3-K/Akt-mediated activation of the NOS-dependent NO production, and SERCA2a pumps in the mechanisms conferring benefits to the goldfish heart under hypoxia. They also propose protein denitrosylation, and the possibility of nitration, as parallel intracellular events.

Sign in / Sign up

Export Citation Format

Share Document